LOCUS       V002733                10163 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002733
VERSION     V002733
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10163)
  AUTHORS   Meissner M, Krejany E, Gilson PR, de Koning-Ward TF, Soldati D,
            Crabb BS.
  TITLE     Tetracycline analogue-regulated transgene expression in Plasmodium
            falciparum blood stages using Toxoplasma gondii transactivators
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 102 (8), 2980-2985 (2005)
   PUBMED   15710888
REFERENCE   2  (bases 1 to 10163)
  AUTHORS   Meissner M, Krejany E, Gilson P, de Koning Ward T, Soldati D, Crabb
            B.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2004) Infection and Immunity, Walter and Eliza
            Hall Institute of Medical Research, Royal Parade, Melbourne,
            Victoria 3050, Australia
REFERENCE   3  (bases 1 to 10163)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10163)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2005"; volume: "102"; issue: "8"; pages:
            "2980-2985"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-DEC-2004) Infection and Immunity, Walter and Eliza Hall
            Institute of Medical Research, Royal Parade, Melbourne, Victoria
            3050, Australia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..1078
                     /label="calmodulin promoter"
                     /note="calmodulin promoter"
                     /regulatory_class="promoter"
     CDS             1079..1639
                     /gene="DHFR"
                     /label="Dihydrofolate reductase"
                     /note="Dihydrofolate reductase from Homo sapiens.
                     Accession#: P00374"
     regulatory      1641..2215
                     /label="HRP23 terminator"
                     /note="HRP23 terminator"
                     /regulatory_class="terminator"
     promoter        complement(2219..2236)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2243..2259)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2733..2837
                     /label="AmpR promoter"
     CDS             2838..3695
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3869..4457
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4745..4766
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4781..4811
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4819..4835
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4843..4859
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4877..4895
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4927..4943)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    4954..5224
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     regulatory      5240..5563
                     /label="minimal calmodulin promoter"
                     /note="minimal calmodulin promoter"
                     /regulatory_class="promoter"
     CDS             5648..6361
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      complement(7151..8075)
                     /note="Plasmodium berghei dihydrofolate reductase
                     terminator"
                     /regulatory_class="terminator"
     CDS             complement(8173..8775)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     regulatory      complement(8776..9652)
                     /note="Plasmodium falciparum merozoite surface protein 2
                     promoter"
                     /regulatory_class="promoter"
     repeat_region   9653..10163
                     /label="REP20 subtelomeric repeat region"
                     /note="REP20 subtelomeric repeat region"
ORIGIN
        1 tataaggaaa ttcccatata taaaaaaaaa agtacctatt aataattata agcttgcatg
       61 caatctctga gcttcttctt tgttaaccat tttgtaaaaa aaattaaaat atatttatat
      121 aatattattt tattttatta tatattatat tatttttatt tttattttta tttttttttc
      181 tctacaaatt ttatctattg gtttattata aaaatatcta tttctaataa taaataatta
      241 agatatcaat ttatagaaac aaaatatata cttgtataat tttatttttt tatataaatc
      301 attacatata taattataca atattttttc taagagataa ttatatatta atatatataa
      361 aaaaaggtgt tttttttttt tttttttatt tttattttta ttttatggta atattttatt
      421 ttccttattt tataaattat attagtttat atgtgattaa ttttatatat tatcaattta
      481 tatattttta aatgcttact taattatctt tttttttttt tttttttttt tttcccctct
      541 ttttatatta atttattttt gaaaaaattg atatatatat atatatataa tatatatata
      601 tacatgtagt agtattaaac aatgtataat atatataaat aatatattta tatatttcat
      661 ttcaatttta attttttttg gttttttttt tttttctttt tgtcatattt aaaaaaaatt
      721 atattcatat aagttatgca ttttttataa acattattca atatatgtat aatataatat
      781 atatatatat attaatgtat tattccaatg tgcatgataa aagaaaaaaa taatatttat
      841 aaaaaaaaag aaaaataaaa caaaaaaaga aaaaaaaaaa aaaaaaaaaa aaaatacaaa
      901 aataaataat ataatttata attatatatt cttgtcacaa taaaaatata tatatatata
      961 tatatattta taatatgtat attttaaact agaaaaggaa taactaatat tttatttatt
     1021 atcattcaag atttatattt tataataata aatacctaat agaaatatat caggatccat
     1081 gcatggttcg ctaaactgca tcgtcgctgt gtcccagaac atgggcatcg gcaagaacgg
     1141 ggactacccc tggccaccgc tcaggaacga atttagatat ttccagagaa tgaccacaac
     1201 ctcttcagta gaaggtaaac agaatctggt gattatgggt aagaagacct ggttctccat
     1261 tcctgagaag aatcgacctt taaagggtag aattaattta gttctcagca gagaactcaa
     1321 ggaacctcca caaggagctc attttctttc cagaagtcta gatgatgcct taaaacttac
     1381 tgaacaacca gaattagcaa ataaagtaga catggtctgg atagttggtg gcagttctgt
     1441 ttataaggaa gccatgaatc acccaggcca tcttaaacta tttgtgacaa ggatcatgca
     1501 agactttgaa agtgacacgt tttttccaga aattgatttg gagaaatata aacttctgcc
     1561 agaataccca ggtgttctct ctgatgtcca ggaggagaaa ggcattaagt acaaatttga
     1621 agtatatgag aagaatgatt aagcttattt aataatagat taaaaatatt ataaaaataa
     1681 aaacataaac acagaaatta caaaaaaaat acatatgaat tttttttttg taatcttcct
     1741 tataaatata gaataatgaa tcatataaaa catatcatta ttcatttatt tacatttaaa
     1801 attattgttt cagtatcttt aatttattat gtatatataa aaataactta caattttatt
     1861 aataaacaat atatgtttat taattcatgt tttgtaattt atgggatagc gatttttttt
     1921 actgtctgta tttttctttt ttaattatgt tttaattgta ttttattttt attattgttc
     1981 tttttatagt attattttaa aacaaaatgt attttctaag aacttataat aataataaat
     2041 ataaatttta ataaaaatta tatttatctt ttacaatatg aacataaagt acaacattaa
     2101 tatatagctt ttaatatttt tattcctaat catgtaaatc ttaaattttt ctttttaaac
     2161 atatgttaaa tatttatttc tcattatata taagaacata tttattaaat ctagaattct
     2221 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2281 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2341 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     2401 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     2461 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     2521 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     2581 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     2641 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     2701 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     2761 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2821 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     2881 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     2941 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     3001 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3061 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     3121 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     3181 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     3241 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     3301 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     3361 agcgtgacac cacgatgcct gtagcaatgc caacaacgtt gcgcaaacta ttaactggcg
     3421 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     3481 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     3541 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     3601 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     3661 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     3721 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3781 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3841 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3901 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3961 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     4021 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4081 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4141 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4201 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4261 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4321 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4381 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4441 ggcggagcct atcgaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4501 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4561 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4621 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4681 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4741 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4801 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4861 atgattacgc caagctattt aggtgacact atagaatact cagaagaatg attaagcggc
     4921 cgctttcgat accgtcgacc tcgagtttac cactccctat cagtgataga gaaaagtgaa
     4981 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     5041 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     5101 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     5161 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     5221 gagaaaagtg aaagtcgagc tcggtaccca attcgcccta tagtgagtcg cagcatgcat
     5281 gtattattcc aatgtgcatg ataaaagaaa aaaataatat ttataaaaaa aaagaaaaat
     5341 aaaacaaaaa aagaaaaaaa aaaaaaaaaa aaaaaaaata caaaaataaa taatataatt
     5401 tataattata tattcttgtc acaataaaaa tatatatata tatatatata tttataatat
     5461 gtatatttta aactagaaaa ggaataacta atattttatt tattatcatt caagatttat
     5521 attttataat aataaatacc taatagaaat atatcaggat cgccagctgc aggtcaaaat
     5581 gaagatcata ttctttttat gttcatttct tttttttatt ataaatacac aatgtgtaac
     5641 acatgaaatg agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     5701 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     5761 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     5821 aacacttgtc actactttcg cgtatggtct tcaatgcttt gcgagatacc cagatcatat
     5881 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
     5941 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     6001 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
     6061 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
     6121 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
     6181 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     6241 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     6301 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     6361 aaatgttgaa aaagatgaag cacatgttaa aataactaaa cttagtgatt taaaagcaat
     6421 tgtattctgt agttcttccg gatttatggg attatcaatt ttattgatca tcacattaat
     6481 tgtatttaat ttattttaaa ctagttttta aatatgcaga ataaaataaa taagtaaata
     6541 tcatacgttt atatgtatat atatatatat atatatattt gagacgtata ttttatatat
     6601 gcattatata tacattcgta taaatatacg tgcatgtaac tttttctata ttattattat
     6661 tttttttata tttattatca tttttttttt tttcttatgt atttttaata tatatatata
     6721 tatatatatt tatttattta tttattagta gataattttt tttatcttgt tttaactgaa
     6781 ataattctaa attttcttaa atgcaggaac aaaatataaa cggtttatgg ttttatttat
     6841 ttatattata tatatgaaaa atataatttt tgttccattt acacataaat gtcacacaaa
     6901 agagaaataa cataaatgtt tatatttttt ttaacctgcc aatttttaga agcataaaat
     6961 gttatatttt taccaaaaaa aaaaaaaaaa taaataaata aataaatgaa atataataaa
     7021 aatataatta aaatatataa taataataat aaataaatgt aaggcacatt tttaaaagac
     7081 aatatttata agtccattta acattattat ttgagcgtac attactcctc ttttttatta
     7141 agtgatcgat gcggccgcct accctgaaga agaaaagtcc gatgatgttg tggacataaa
     7201 gaattgagct gtctcaaaaa atgagaaaac aaacaaacaa acaaaatacc attatgcgca
     7261 tatcctctgc tcaatatcaa tttttcttta tgccagtttt gtttcgacac tatttgagca
     7321 acaccttccc tttactatct actactgtta caatgttatt ttattcacct ttttacaatt
     7381 ttatcaaaat ttttttgtat gcatataaat tatacattgt aaagccaaag tgtagataaa
     7441 gacaccttgt ttatttttcc ccaatttttt ttttttacca tttttttatt aaccttgctc
     7501 atttacctgc taatacgatt gctatatgat atcgaaattg aaggaaaaac catcatttgt
     7561 gtttatatgt ttttttttaa tttttcaact ttttttattt aactatgcta atcatattat
     7621 aaacatttta aaaatcaagt ccaactattt atgaatcatt gaaggagaca cataattata
     7681 acagtaaaaa taataataat aataataatt ttgtcttatt gtttcaaaca tattatgttc
     7741 cataaatatc taaattttta atatggtcat aaattcatag gatttaattt atataaaata
     7801 tggctattca tactagccat tttatgtgtg taaagttttt tttttctgaa caaaaaataa
     7861 aatgttcata attttagcta tttacatgca tgtgcatgca caaaaaaaaa tatgcacaca
     7921 acatacacat ttttacagtt ataaatacaa tcaattggta taaatatata aataagaaaa
     7981 acgaacatta agctgccata tccctcgacc ctaggtgatt aattaagcgt taatattttg
     8041 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     8101 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     8161 tggaacaaga gtcagctgtt tctccaggcc acatatgatt agttccaggc cgaaaaggaa
     8221 ggcaggttcg gctccctgat ggtcgaacag ctcaattgct tgtctcagaa gtgggggcat
     8281 agaatcggtg gtaggtgtct ctctttcctc ttttgctact tgatgctcct gatcctccaa
     8341 tacgcagccc agtgtaaagt ggcccacggc ggacagagcg tacagtgcgt tctccaggga
     8401 gaagccttgc tgacacagga acgcgagctg attttccagg gtttcgtact gtttctctgt
     8461 tgggcgggtg ccgagatgca ctttagcccc gtcgcgatgt gagaggagag cacagcggaa
     8521 tgacttggcg ttgttccgca gaaagtcttg ccatgactcg ccttccaggg ggcagaagtg
     8581 ggtatgatgc ctgtccagca tctcgattgc cagggcatcg agcagggccc gcttgttctt
     8641 cacgtgccag tacagggtag gctgctcaac tcccagcttt tgagcgagtt tccttgtcgt
     8701 caggccttcg ataccgactt cattgagtaa ttccagagca gagttgatga ctttgctctt
     8761 gtccaggcgc gacattctcg agtttattcg aacttaagtt tgactaatat aatatgttaa
     8821 gaattaataa atacaattct tttaaattaa ttattaaaaa atattaaatt acaaaatgaa
     8881 atttaatata attaatattt tattattatt aataaatgaa taaatattta atatttactt
     8941 aaatatatat acatatatat atatatatat tttttccttt atgattaact tatgaatata
     9001 ttttttttta taaatagttc tatttctttt cttttttttt ttatatttgt gttgtttttt
     9061 ctatatgtct aaaaatatat aaattaaaaa aatttatata atgaaaaaaa tggaaattgt
     9121 aaattgatta gtcgagattt acttatatat aattcatatg tttattagaa aaaaaaaaca
     9181 aaaaaaagaa aaaagaaaaa acatttttta tgtttttttt tttttttgtg tgtgcatttg
     9241 atttattata tgaaaagata ctaggtttta tatcgtaatt gattaaaata aatattaatg
     9301 caccttaaaa tgaaaatgaa attaaaaaaa aaaaaaagag tcttactgca catatattta
     9361 ttatatattt ataatatatt tattatatat ttattatata tttataatat atttattata
     9421 tatttattat atatttatta tatatttata atatttatat tatattatat tatatttata
     9481 ttacatttat attatattta tattatattt atattatatt tatctattat ttttttgata
     9541 atatggtgaa aaattatccg agggataata agtgtgcaat aataatagta ttgaataaat
     9601 aaaataatat taaatttggt aagattttta attatatttg ttatacccgg gagatcttac
     9661 tttcactaat ataggtccta tcataactaa cataggactt atcttaacta actctggtcc
     9721 tactattact aacttaagtc ttactttgcc taagtgaggt cataatttaa ctaatatagg
     9781 tcttaaggtt actaactaag gcccttctct cactcatata ggtcttatgg ttactaacta
     9841 aggtcttact ctcactcata taggtcttat gtttagtaac ttaggtccta acttaactaa
     9901 tataggtcat aagattacta acctaagtca ttaaggtact aacttaggtc gtaaggtaac
     9961 taatatatag gtcttaaggt aactaattta ggtcttgact taataaatat aggtcctaac
    10021 ataaatagta taggtcctaa tataagtact ataggtctta acttaaccaa cataggtcct
    10081 aacataagtt atataggtct taacgtaact aacataagtc attaaggtac taagtttggt
    10141 cttaatttaa caaatataga tct
//
