Basic Vector Information
pTcR-GA-PAC-plus vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTcR-GA-PAC-plus vector Sequence
LOCUS 40924_42864 3574 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pTcR-GA-PAC-plus, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3574) AUTHORS Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira CA. TITLE Plasmids and molecular building blocks to assist the development and extension of genetic manipulation tools for Trypanosoma cruzi JOURNAL Unpublished REFERENCE 2 (bases 1 to 3574) AUTHORS Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira CA. TITLE Direct Submission JOURNAL Submitted (18-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina REFERENCE 3 (bases 1 to 3574) TITLE Direct Submission REFERENCE 4 (bases 1 to 3574) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (18-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..3574 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 568..1164 /codon_start=1 /label=PuroR /note="puromycin N-acetyltransferase" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVEVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPEHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" rep_origin complement(1853..2441) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2615..3472) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(join(3473..3574,1..3)) /label=AmpR promoter
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