LOCUS       40924_42864        3574 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTcR-GA-PAC-plus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 3574)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3574)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             568..1164
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVEVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPEHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     rep_origin      complement(1853..2441)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2615..3472)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(join(3473..3574,1..3))
                     /label=AmpR promoter
ORIGIN
        1 gcggccgcga tatcggtacc atggtcgact gcagatctag aattcgaagc ttaggcgtgg
       61 cgatgacttc aggtctttct tttgcgaata gggatcttat aatacacgat gcgtgtcccg
      121 tgatgatcgt taccggtgct gccacgatcc aactgacaca gcgtcaagag caaaacaatt
      181 ttacttttcc ctttaaggac aacaacaaaa aaatatataa cttttttttc tttttttttt
      241 tttttgaaat tatatttatg gtcatctttg ggaaacaaaa agcagcaatt taatgatgcg
      301 gaaggatgag tgaaataatg tttaatcaat gtacgaggat ttggggtatt gcaaggaaaa
      361 tgtagatgat ttaattgggt gtgtgatgca gcttgtggta atttttgctc acttcccttt
      421 ttgccacatc tttttagttt ttctgctttt ctttccccat tattccactt gtctctcttt
      481 tcccacgttt cctgcacgaa tgcagaaagt gatattttta ctttgaaagc catctaccaa
      541 caacaattac attgaacaga atttaaaatg accgagtaca agcccacggt gcgcctcgcc
      601 acccgcgacg acgtcccccg ggccgtacgc accctcgcag ccgcgttcgc cgactacccc
      661 gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa
      721 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
      781 gcggtggagg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
      841 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
      901 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
      961 cccgaacacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     1021 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     1081 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     1141 tgcatgaccc gcaagcccgg tgcctagtaa tctctacata aaggtgaata gttttcctat
     1201 caccggaatt atttttcttc cgtgggctta ccgttttctg tccttgtaag gtttctgaga
     1261 tcgcgttccc atatatatat atcttttttt caagtgttga tttactgaag gagcttttaa
     1321 taataatttc aagccagtca taatcggaat ctgaacattc aagagatttt ttttttcaaa
     1381 ccttcctcca tgtttgcgtg agcgagctag tcgataggag tttgcagctt tgagctttga
     1441 tggcttgttg gtggtcgttg actttttttt tgtgtgtgat gctcaccctc acggcggttt
     1501 gatctttgtc catttcttgt tttttcttct tcttttttca cataacaaac aaaccaggga
     1561 gaaaaacttt tttaggtttt ttgtttatat cgcgtttttt ttttttttgt ttctaagctt
     1621 catgatcgat gcatgtacaa ttgctagctc gaggatccag ctgcggccgc tcttccgctt
     1681 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1741 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1801 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1861 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1921 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1981 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2041 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2101 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2161 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2221 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2281 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2341 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2401 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2461 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2521 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2581 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2641 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2701 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2761 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2821 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2881 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2941 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3001 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3061 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3121 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3181 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3241 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3301 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3361 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3421 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3481 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3541 aatgtattta gaaaaataaa caaatagggg ttcc
//
