pTARG vector (V002809)

LOCUS       40924_42679        7664 bp DNA     circular SYN 18-DEC-2018
DEFINITION  P element transformation vector pTARG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Egli D, Yepiskoposyan H, Selvaraj A, Balamurugan K, Rajaram R, 
            Simons A, Multhaup G, Mettler S, Vardanyan A, Georgiev O, Schaffner 
            W.
  TITLE     A family knockout of all four Drosophila metallothioneins reveals a 
            central role in copper homeostasis and detoxification
  JOURNAL   Mol. Cell. Biol. 26 (6), 2286-2296 (2006)
  PUBMED    16508004
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Egli D, Georgiev O, Schaffner W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2005) Institute of Molucular Biology, University 
            of Zurich, Winterthurer Street 190, Zurich 8057, Switerland
REFERENCE   3  (bases 1 to 7664)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7664)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Cell. 
            Biol."; date: "2006"; volume: "26"; issue: "6"; pages: "2286-2296"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-OCT-2005) Institute of Molucular Biology, University of Zurich, 
            Winterthurer Street 190, Zurich 8057, Switerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..8
                     /label=Polylinker
                     /note="Polylinker"
     protein_bind    20..67
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    94..127
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    143..264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    476..523
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      720..945
                     /label=3xP3-hsp70 promoter
                     /note="3xP3-hsp70 promoter"
                     /regulatory_class="promoter"
     protein_bind    complement(951..984)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1178..3238
                     /label=mini-white
                     /note="Drosophila white gene eye color pigment"
     misc_feature    3900..3952
                     /label=flippase recombinase target
                     /note="flippase recombinase target"
     protein_bind    3905..3952
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    complement(4086..4671)
                     /label=P element 5' end
     rep_origin      complement(4906..5494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5668..6525)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6526..6630)
                     /label=AmpR promoter
     misc_feature    complement(7432..7664)
                     /label=P element 3' end
                     /note="P element 3' end"