LOCUS       40924_42679        7664 bp DNA     circular SYN 18-DEC-2018
DEFINITION  P element transformation vector pTARG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Egli D, Yepiskoposyan H, Selvaraj A, Balamurugan K, Rajaram R, 
            Simons A, Multhaup G, Mettler S, Vardanyan A, Georgiev O, Schaffner 
            W.
  TITLE     A family knockout of all four Drosophila metallothioneins reveals a 
            central role in copper homeostasis and detoxification
  JOURNAL   Mol. Cell. Biol. 26 (6), 2286-2296 (2006)
  PUBMED    16508004
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Egli D, Georgiev O, Schaffner W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2005) Institute of Molucular Biology, University 
            of Zurich, Winterthurer Street 190, Zurich 8057, Switerland
REFERENCE   3  (bases 1 to 7664)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7664)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Cell. 
            Biol."; date: "2006"; volume: "26"; issue: "6"; pages: "2286-2296"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-OCT-2005) Institute of Molucular Biology, University of Zurich, 
            Winterthurer Street 190, Zurich 8057, Switerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..8
                     /label=Polylinker
                     /note="Polylinker"
     protein_bind    20..67
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    94..127
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    143..264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    476..523
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      720..945
                     /label=3xP3-hsp70 promoter
                     /note="3xP3-hsp70 promoter"
                     /regulatory_class="promoter"
     protein_bind    complement(951..984)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1178..3238
                     /label=mini-white
                     /note="Drosophila white gene eye color pigment"
     misc_feature    3900..3952
                     /label=flippase recombinase target
                     /note="flippase recombinase target"
     protein_bind    3905..3952
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    complement(4086..4671)
                     /label=P element 5' end
     rep_origin      complement(4906..5494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5668..6525)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6526..6630)
                     /label=AmpR promoter
     misc_feature    complement(7432..7664)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 aagcttggct gcaagctttg aagttcctat tccgaagttc ctattctcta gaaagtatag
       61 gaacttcaga gcgctttgca ggtcgacctc gagataactt cgtatagcat acattatacg
      121 aagttatccg gtcgccacca tgaacttgtt tattgcagct tataatggtt acaaataaag
      181 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      241 gtccaaactc atcaatgtat cttaaagctt atcgatacgc gttctagact cgaggctgca
      301 ggcctcgtac gcggccgccg cgtgcgcgcg aattcaccgg tggtaaccag atctgctagc
      361 cctagggtcg acccgcggac tagtggcgcg cccaaaacgt cgtgagacag tttggtattc
      421 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
      481 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
      541 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
      601 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
      661 aacgtcgggg cggcaggccc tgccatagcc tcaggttact caattcgagc tcgcccgggg
      721 atctaattca attagagact aattcaatta gagctaattc aattaggatc caagcttatc
      781 gatttcgaac cctcgaccgc cggagtataa atagaggcgc ttcgtctacg gagcgacaat
      841 tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg
      901 cagctgaaca agctaaacaa tcggggtacg taccgctaga gtcgatcgac ataacttcgt
      961 atagcataca ttatacgaag ttatgagctc aattggtacc gaattaattc ggcacgagcg
     1021 gtttcgtgac gaagctccaa gcggtttacg ccatcaatta aacacaaagt gctgtgccaa
     1081 aactcctctc gcttcttatt tttgtttgtt ttttgagtga ttgggtgggt gattggtttt
     1141 gggtgggtaa gcagggaaag tgtgaaaaat cccggcaatg ggccaagagg atcaggagct
     1201 attaattcgc ggaggcagca aacacccatc tgccgagcat ctgaacaatg gtgacagcgg
     1261 agcggcttcg cagagctgca ttaaccaggg cttcgggcag gccaaaaact acggcacgct
     1321 cctgccaccc agtccgccgg aggactccgg ttcagggagc ggccaactag ccgagaacct
     1381 cacctatgcc tggcacaata tggacatctt tggggcggtc aatcagccgg gctccggatg
     1441 gcggcagctg gtcaaccgga cacgcggact attctgcaac gagcgacaca taccggcgcc
     1501 caggaaacat ttgctcaaga acgtttgcgg cgtggcctat ccgggcgaac ttttggccgt
     1561 gatgggcagt tccggtgccg gaaagacgac cctgctgaat gcccttgcct ttcgatcgcc
     1621 gcagggcatc caagtatcgc catccgggat gcgactgctc aatggccaac ctgtggacgc
     1681 caaggagatg caggccaggt gcgcctatgt ccagcaggat gacctcttta tcggctccct
     1741 aacggccagg gaacacctga ttttccaggc catggtgcgg atgccacgac atctgaccta
     1801 tcggcagcga gtggcccgcg tggatcaggt gatccaggag ctttcgctca gcaaatgtca
     1861 gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg tccggcggag aaaggaagcg
     1921 tctggcattc gcctccgagg cactaaccga tccgccgctt ctgatctgcg atgagcccac
     1981 ctccggactg gactcattta ccgcccacag cgtcgtccag gtgctgaaga agctgtcgca
     2041 gaagggcaag accgtcatcc tgaccattca tcagccgtct tccgagctgt ttgagctctt
     2101 tgacaagatc cttctgatgg ccgagggcag ggtagctttc ttgggcactc ccagcgaagc
     2161 cgtcgacttc ttttcctacg tgggtgccca gtgtcctacc aactacaatc cggcggactt
     2221 ttacgtacag gtgttggccg ttgtgcccgg acgggagatc gagtcccgtg atcggatcgc
     2281 caagatatgc gacaattttg ccattagcaa agtagcccgg gatatggagc agttgttggc
     2341 caccaaaaat ttggagaagc cactggagca gccggagaat gggtacacct acaaggccac
     2401 ctggttcatg cagttccggg cggtcctgtg gcgatcctgg ctgtcggtgc tcaaggaacc
     2461 actcctcgta aaagtgcgac ttattcagac aacgatggtt gccatcttga ttggcctcat
     2521 ctttttgggc caacaactca cgcaagtggg cgtgatgaat atcaacggag ccatcttcct
     2581 cttcctgacc aacatgacct ttcaaaacgt ctttgccacg ataaatgtgt tcacctcaga
     2641 gctgccagtt tttatgaggg aggcccgaag tcgactttat cgctgtgaca catactttct
     2701 gggcaaaacg attgccgaat tgccgctttt tctcacagtg ccactggtct tcacggcgat
     2761 tgcctatccg atgatcggac tgcgggccgg agtgctgcac ttcttcaact gcctggcgct
     2821 ggtcactctg gtggccaatg tgtcaacgtc cttcggatat ctaatatcct gcgccagctc
     2881 ctcgacctcg atggcgctgt ctgtgggtcc gccggttatc ataccattcc tgctctttgg
     2941 cggcttcttc ttgaactcgg gctcggtgcc agtatacctc aaatggttgt cgtacctctc
     3001 atggttccgt tacgccaacg agggtctgct gattaaccaa tgggcggacg tggagccggg
     3061 cgaaattagc tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg tcatcctgga
     3121 gacgcttaac ttctccgccg ccgatctgcc gctggactac gtgggtctgg ccattctcat
     3181 cgtgagcttc cgggtgctcg catatctggc tctaagactt cgggcccgac gcaaggagta
     3241 gccgacatat atccgaaata actgcttgtt ttttttttta ccattattac catcgtgttt
     3301 actgtttatt gccccctcaa aaagctaatg taattatatt tgtgccaata aaaacaagat
     3361 atgacctata gaatacaagt atttcccctt cgaacatccc cacaagtaga ctttggattt
     3421 gtcttctaac caaaagactt acacacctgc ataccttaca tcaaaaactc gtttatcgct
     3481 acataaaaca ccgggatata ttttttatat acatactttt caaatcgcgc gccctcttca
     3541 taattcacct ccaccacacc acgtttcgta gttgctcttt cgctgtctcc cacccgctct
     3601 ccgcaacaca ttcacctttt gttcgacgac cttggagcga ctgtcgttag ttccgcgcga
     3661 ttcggttcgc tcaaatggtt ccgagtggtt catttcgtct caatagaaat tagtaataaa
     3721 tatttgtatg tacaatttat ttgctccaat atatttgtat atatttccct cacagctata
     3781 tttattctaa tttaatatta tgacttttta aggtaatttt ttgtgacctg ttcggagtga
     3841 ttagcgttac aatttgaact gaaagtgaca tccagtgttt gttccttgtg tagatgcaag
     3901 ctttgaagtt cctattccga agttcctatt ctctagaaag tataggaact tcagagcgct
     3961 ttgcatctca aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac
     4021 tataataata agaatacatt taatttagaa aatgcttgga tttcactgga actagaatta
     4081 attcggctgc tgctctaaac gacgcatttc gtactccaaa gtacgaattt tttccctcaa
     4141 gctcttattt tcattaaaca atgaacagga cctaacgcac agtcacgtta ttgtttacat
     4201 aaatgatttt ttttactatt caaacttact ctgtttgtgt actcccactg gtatagcctt
     4261 cttttatctt ttctggttca ggctctatca ctttactagg tacggcatct gcgttgagtc
     4321 gcctcctttt aaatgtctga ccttttgcag gtgcagcctt ccactgcgaa tctttaaagt
     4381 gggtatcaca aatttgggag ttttcaccaa ggctgcaccc aaggctctgc tcccacaatt
     4441 ttctcttaat agcacacttc ggcacgtgaa ttaattttac tccagtcaca gctttgcagc
     4501 aaaatttgca atatttcatt tttttttatt ccacgtaagg gttaatgttt tcaaaaaaaa
     4561 attcgtccgc acacaacctt tcctctcaac aagcaaacgt gcactgaatt taagtgtata
     4621 cttcggtaag cttcggctat cgacgggacc accttatgtt atttcatcat gggccagacc
     4681 cacgtagtcc agcggcagat cggcggcgga gaagttaagc gtctccagga tgaccttgcc
     4741 cgaactgggg cacgtggtgt tcgacgatgt gcagctaatt tcgcccggct ccacgtccgc
     4801 ccattggtta atcagcagac cctcgttggc gtaacggaac catgagaggt acgacaacca
     4861 tttgaggtat actggcaccg agcccgagtt caagaagaag gcgtttttcc ataggctccg
     4921 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4981 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5041 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5101 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5161 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5221 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5281 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5341 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5401 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5461 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5521 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5581 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5641 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5701 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5761 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5821 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5881 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5941 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     6001 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     6061 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     6121 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     6181 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     6241 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     6301 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     6361 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6421 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6481 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6541 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6601 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     6661 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     6721 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     6781 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     6841 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     6901 agtgcaccat atgcggtgtg aaataccgca ccgaatcgcg cggaactaac gacagtcgct
     6961 ccaaggtcgt cgaacaaaag gtgaatgtgt tgcggagagc gggtgggaga cagcgaaaga
     7021 gcaactacga aacgtggtgt ggtggaggtg aattatgaag agggcgcgcg atttgaaaag
     7081 tatgtatata aaaaatatat cccggtgttt tatgtagcga taaacgagtt tttgatgtaa
     7141 ggtatgcagg tgtgtaagtc ttttggttag aagacaaatc caaagtctac ttgtggggat
     7201 gttcgaaggg gaaatacttg tattctatag gtcatatctt gtttttattg gcacaaatat
     7261 aattacatta gctttttgag ggggcaataa acagtaaaca cgatggtaat aatggtaaaa
     7321 aaaaaaacaa gcagttattt cggatatatg tcggctactc cttgcgtcgg gcccgaagtc
     7381 ttagagccag atatgcgagc acccggaagc tcacgatgag aatggccaga ccatgatgaa
     7441 ataacataag gtggtcccgt cggcaagaga catccactta acgtatgctt gcaataagtg
     7501 cgagtgaaag gaatagtatt ctgagtgtcg tattgagtct gagtgagaca gcgatatgat
     7561 tgttgattaa cccttagcat gtccgtgggg tttgaattaa ctcataatat taattagacg
     7621 aaattatttt taaagtttta tttttaataa tttgcgagta cgca
//
