pTARBAC6 vector (V002810)

LOCUS       V002810                13407 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002810
VERSION     V002810
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13407)
  AUTHORS   Osoegawa K, Vessere GM, Li Shu C, Hoskins RA, Abad JP, de Pablos B,
            Villasante A, de Jong PJ.
  TITLE     BAC clones generated from sheared DNA
  JOURNAL   Genomics 89 (2), 291-299 (2007)
   PUBMED   17098394
REFERENCE   2  (bases 1 to 13407)
  AUTHORS   Osoegawa K, De Jong PJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2006) Children's Hospital and Research Center at
            Oakland, 747 52nd Street, Oakland, CA 94609, USA
REFERENCE   3  (bases 1 to 13407)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13407)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genomics";
            date: "2007"; volume: "89"; issue: "2"; pages: "291-299"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (25-OCT-2006) Children's Hospital and Research Center at Oakland,
            747 52nd Street, Oakland, CA 94609, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(115..131)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(139..155)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(163..193)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(208..229)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(517..1105)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1279..2136)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2137..2241)
                     /label="AmpR promoter"
     primer_bind     2715..2731
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(2757..2775)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             2875..4290
                     /gene="sacB"
                     /label="Levansucrase"
                     /note="Levansucrase from Bacillus amyloliquefaciens.
                     Accession#: P21130"
     promoter        5243..5430
                     /label="HIS3 promoter"
     CDS             5431..6090
                     /label="HIS3"
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     promoter        complement(6565..6593)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     misc_feature    complement(6925..7398)
                     /label="sopC"
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(7474..8442)
                     /label="sopB"
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             complement(8445..9617)
                     /label="sopA"
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    complement(9943..10193)
                     /label="incC"
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(10199..10951)
                     /label="repE"
                     /note="replication initiation protein for the bacterial F
                     plasmid"
     rep_origin      complement(11042..11261)
                     /direction=LEFT
                     /label="ori2"
                     /note="secondary origin of replication for the bacterial F
                     plasmid; also known as oriS"
     promoter        12188..12290
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             12291..12947
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     protein_bind    complement(13199..13232)
                     /label="loxP511"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     promoter        13380..13398
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"