LOCUS       V002810                13407 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002810
VERSION     V002810
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13407)
  AUTHORS   Osoegawa K, Vessere GM, Li Shu C, Hoskins RA, Abad JP, de Pablos B,
            Villasante A, de Jong PJ.
  TITLE     BAC clones generated from sheared DNA
  JOURNAL   Genomics 89 (2), 291-299 (2007)
   PUBMED   17098394
REFERENCE   2  (bases 1 to 13407)
  AUTHORS   Osoegawa K, De Jong PJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2006) Children's Hospital and Research Center at
            Oakland, 747 52nd Street, Oakland, CA 94609, USA
REFERENCE   3  (bases 1 to 13407)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13407)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genomics";
            date: "2007"; volume: "89"; issue: "2"; pages: "291-299"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (25-OCT-2006) Children's Hospital and Research Center at Oakland,
            747 52nd Street, Oakland, CA 94609, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(115..131)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(139..155)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(163..193)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(208..229)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(517..1105)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1279..2136)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2137..2241)
                     /label="AmpR promoter"
     primer_bind     2715..2731
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(2757..2775)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             2875..4290
                     /gene="sacB"
                     /label="Levansucrase"
                     /note="Levansucrase from Bacillus amyloliquefaciens.
                     Accession#: P21130"
     promoter        5243..5430
                     /label="HIS3 promoter"
     CDS             5431..6090
                     /label="HIS3"
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     promoter        complement(6565..6593)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     misc_feature    complement(6925..7398)
                     /label="sopC"
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(7474..8442)
                     /label="sopB"
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             complement(8445..9617)
                     /label="sopA"
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    complement(9943..10193)
                     /label="incC"
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(10199..10951)
                     /label="repE"
                     /note="replication initiation protein for the bacterial F
                     plasmid"
     rep_origin      complement(11042..11261)
                     /direction=LEFT
                     /label="ori2"
                     /note="secondary origin of replication for the bacterial F
                     plasmid; also known as oriS"
     promoter        12188..12290
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             12291..12947
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     protein_bind    complement(13199..13232)
                     /label="loxP511"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     promoter        13380..13398
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tccattgtgt tgggaattcg agctcacgcg tactgatgca tgatccgggt ttaaacccag
       61 tactctagat cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata
      121 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      181 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      241 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      301 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      361 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      421 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      481 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      541 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      601 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      661 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
      721 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      781 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      841 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      901 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
      961 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1021 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1081 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1141 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1201 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1261 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1321 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1381 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     1441 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     1501 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     1561 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     1621 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     1681 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     1741 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     1801 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     1861 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     1921 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     1981 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2041 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     2101 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     2161 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     2221 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     2281 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     2341 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     2401 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     2461 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     2521 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     2581 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2641 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2701 ccagtcacga cgttgtaaaa cgacggccag tgaattccca acacaatgga gatccttcta
     2761 tagtgtcacc taaatgtcga cggccaggcg gccgccaggc ctacccacta gtcaattcgg
     2821 gaggatcgaa acggcagatc gcaaaaaaca gtacatacag aaggagacat gaacatgaac
     2881 atcaaaaaaa ttgtaaaaca agccacagtt ctgactttta cgactgcact tctggcagga
     2941 ggagcgactc aagccttcgc gaaagaaaat aaccaaaaag catacaaaga aacgtacggc
     3001 gtctctcata ttacacgcca tgatatgctg cagatcccta aacagcagca aaacgaaaaa
     3061 taccaagtgc ctcaattcga tcaatcaacg attaaaaata ttgagtctgc aaaaggactt
     3121 gatgtgtggg acagctggcc gctgcaaaac gctgacggaa cagtagctga atacaacggc
     3181 tatcacgttg tgtttgctct tgcgggaagc ccgaaagacg ctgatgacac atcaatctac
     3241 atgttttatc aaaaggtcgg cgacaactca atcgacagct ggaaaaacgc gggccgtgtc
     3301 tttaaagaca gcgataagtt cgacgccaac gatccgatcc tgaaagatca gacgcaagaa
     3361 tggtccggtt ctgcaacctt tacatctgac ggaaaaatcc gtttattcta cactgactat
     3421 tccggtaaac attacggcaa acaaagcctg acaacagcgc aggtaaatgt gtcaaaatct
     3481 gatgacacac tcaaaatcaa cggagtggaa gatcacaaaa cgatttttga cggagacgga
     3541 aaaacatatc agaacgttca gcagtttatc gatgaaggca attatacatc cggcgacaac
     3601 catacgctga gagaccctca ctacgttgaa gacaaaggcc ataaatacct tgtattcgaa
     3661 gccaacacgg gaacagaaaa cggataccaa ggcgaagaat ctttatttaa caaagcgtac
     3721 tacggcggcg gcacgaactt cttccgtaaa gaaagccaga agcttcagca gagcgctaaa
     3781 aaacgcgatg ctgagttagc gaacggcgcc ctcggtatca tagagttaaa taatgattac
     3841 acattgaaaa aagtaatgaa gccgctgatc acttcaaaca cggtaactga tgaaatcgag
     3901 cgcgcgaatg ttttcaaaat gaacggcaaa tggtacttgt tcactgattc acgcggttca
     3961 aaaatgacga tcgatggtat taactcaaac gatatttaca tgcttggtta tgtatcaaac
     4021 tctttaaccg gcccttacaa gccgctgaac aaaacagggc ttgtgctgca aatgggtctt
     4081 gatccaaacg atgtgacatt cacttactct cacttcgcag tgccgcaagc caaaggcaac
     4141 aatgtggtta tcacaagcta catgacaaac agaggcttct tcgaggataa aaaggcaaca
     4201 tttgcgccaa gcttcttaat gaacatcaaa ggcaataaaa catccgttgt caaaaacagc
     4261 atcctggagc aaggacagct gacagtcaac taataacagc aaaaagaaaa tgccgatact
     4321 tcattggcat tttcttttat ttctcaacaa gatggtgaat tgactagtgg gtagatccac
     4381 aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc
     4441 aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat
     4501 tgcatcaacg catatagcgc tagattattg aagcatttat cagggttatt gtctcatgag
     4561 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4621 ccgaaaagtg ccacctgacg gatcgcttgc ctgtaactta cacgcgcctc gtaggcctga
     4681 gagcaggaag agcaagataa aaggtagtat ttgttggcga tccccctaga gtcttttaca
     4741 tcttcggaaa acaaaaacta ttttttcttt aatttctttt tttactttct atttttaatt
     4801 tatatattta tattaaaaaa tttaaattat aattattttt atagcacgtg atgaaaagga
     4861 ccctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     4921 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     4981 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     5041 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     5101 agaatgcacc ataattcccg ttttaagagc ttggtgagcg ctaggagtca ctgccaggta
     5161 tcgtttgaac acggcattag tcagggaagt cataacacag tcctttcccg caattttctt
     5221 tttctattac tcttggcctc ctctagtaca ctctatattt ttttatgcct cggtaatgat
     5281 tttcattttt ttttttccac ctagcggatg actctttttt tttcttagcg attggcatta
     5341 tcacataatg aattatacat tatataaagt aatgtgattt cttcgaagaa tatactaaaa
     5401 aatgagcagg caagataaac gaaggcaaag atgacagagc agaaagccct agtaaagcgt
     5461 attacaaatg aaaccaagat tcagattgcg atctctttaa agggtggtcc cctagcgata
     5521 gagcactcga tcttcccaga aaaagaggca gaagcagtag cagaacaggc cacacaatcg
     5581 caagtgatta acgtccacac aggtataggg tttctggacc atatgataca tgctctggcc
     5641 aagcattccg gctggtcgct aatcgttgag tgcattggtg acttacacat agacgaccat
     5701 cacaccactg aagactgcgg gattgctctc ggtcaagctt ttaaagaggc cctaggggcc
     5761 gtgcgtggag taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttctaga
     5821 gcggtggtag atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag
     5881 aaagtaggag atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct
     5941 agcagaatta ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag
     6001 agtgcgttca aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac
     6061 gatgttccct ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca
     6121 tacgatatat atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga
     6181 acagtatgat actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga
     6241 ggcgcgcttt ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc
     6301 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta
     6361 agcgttaatc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc
     6421 gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
     6481 tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
     6541 aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag
     6601 aattgatccg gaacccttaa tataactccg tataatggat gctatacgag gttattaggt
     6661 ccctcgacta tagggtcacc gtcgacagcg acacacttgc atcggatgca gcccggttaa
     6721 cgtgccggca cggcctgggt aaccaggtat tttgtccaca taaccgtgcg caaaatgttg
     6781 tggataagca ggacacagca gcaatccaca gcaggcatac aaccgcacac cgaggttact
     6841 ccgttctaca ggttacgacg acatgtcaat acttgccctt gacaggcatt gatggaatcg
     6901 tagtctcacg ctgatagtct gatcgacaat acaagtggga ccgtggtccc agaccgataa
     6961 tcagaccgac aacacgagtg ggatcgtggt cccagactaa taatcagacc gacgatacga
     7021 gtgggaccgt ggtcccagac taataatcag accgacgata cgagtgggac cgtggttcca
     7081 gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagactaat aatcagaccg
     7141 acgatacgag tgggaccatg gtcccagact aataatcaga ccgacgatac gagtgggacc
     7201 gtggtcccag tctgattatc agaccgacga tacgagtggg accgtggtcc cagactaata
     7261 atcagaccga cgatacgagt gggaccgtgg tcccagacta ataatcagac cgacgatacg
     7321 agtgggaccg tggtcccagt ctgattatca gaccgacgat acaagtggaa cagtgggccc
     7381 agagagaata ttcaggccag ttatgctttc tggcctgtaa caaaggacat taagtaaaga
     7441 cagataaacg tagactaaaa cgtggtcgca tcagggtgct ggcttttcaa gttccttaag
     7501 aatggcctca attttctcta tacactcagt tggaacacga gacctgtcca ggttaagcac
     7561 cattttatcg cccttataca atactgtcgc tccaggagca aactgatgtc gtgagcttaa
     7621 actagttctt gatgcagatg acgttttaag cacagaagtt aaaagagtga taacttcttc
     7681 agcttcaaat atcaccccag cttttttctg ctcatgaagg ttagatgcct gctgcttaag
     7741 taattcctct ttatctgtaa aggctttttg aagtgcatca cctgaccggg cagatagttc
     7801 accggggtga gaaaaaagag caacaactga tttaggcaat ttggcggtgt tgatacagcg
     7861 ggtaataatc ttacgtgaaa tattttccgc atcagccagc gcagaaatat ttccagcaaa
     7921 ttcattctgc aatcggcttg cataacgctg accacgttca taagcacttg ttgggcgata
     7981 atcgttaccc aatctggata atgcagccat ctgctcatca tccagctcgc caaccagaac
     8041 acgataatca ctttcggtaa gtgcagcagc tttacgacgg cgactcccat cggcaatttc
     8101 tatgacacca gatactcttc gaccgaacgc cggtgtctgt tgaccagtca gtagaaaaga
     8161 agggatgaga tcatccagtg cgtcctcagt aagcagctcc tggtcacgtt cattacctga
     8221 ccatacccga gaggtcttct caacactatc accccggagc acttcaagag taaacttcac
     8281 atcccgacca catacaggca aagtaatggc attaccgcga gccattactc ctacgcgcgc
     8341 aattaacgaa tccaccatcg gggcagctgg tgtcgataac gaagtatctt caaccggttg
     8401 agtattgagc gtatgttttg gaataacagg cgcacgcttc attatctaat ctcccagcgt
     8461 ggtttaatca gacgatcgaa aatttcattg cagacaggtt cccaaataga aagagcattt
     8521 ctccaggcac cagttgaaga gcgttgatca atggcctgtt caaaaacagt tctcatccgg
     8581 atctgacctt taccaacttc atccgtttca cgtacaacat tttttagaac catgcttccc
     8641 caggcatccc gaatttgctc ctccatccac ggggactgag agccattact attgctgtat
     8701 ttggtaagca aaatacgtac atcaggctcg aaccctttaa gatcaacgtt cttgagcaga
     8761 tcacgaagca tatcgaaaaa ctgcagtgcg gaggtgtagt caaacaactc agcaggcgtg
     8821 ggaacaatca gcacatcagc agcacatacg acattaatcg tgccgatacc caggttaggc
     8881 gcgctgtcaa taactatgac atcatagtca tgagcaacag tttcaatggc cagtcggagc
     8941 atcaggtgtg gatcggtggg cagtttacct tcatcaaatt tgcccattaa ctcagtttca
     9001 atacggtgca gagccagaca ggaaggaata atgtcaagcc ccggccagca agtgggcttt
     9061 attgcataag tgacatcgtc cttttcccca agatagaaag gcaggagagt gtcttctgca
     9121 tgaatatgaa gatctggtac ccatccgtga tacattgagg ctgttccctg ggggtcgtta
     9181 ccttccacga gcaaaacacg tagccccttc agagccagat cctgagcaag atgaacagaa
     9241 actgaggttt tgtaaacgcc gcctttatgg gcagcaaccc cgatcaccgg tggaaatacg
     9301 tcttcagcac gtcgcaatcg cgtaccaaac acatcacgca tatgattaat ttgttcaatt
     9361 gtataaccaa cacgttgctc aacccgtcct cgaatttcca tatccgggtg cggtagtcgc
     9421 cctgctttct cggcatctct gatagcctga gaagaaaccc caactaaatc cgctgcttca
     9481 cctattctcc agcgccgggt tattttcctc gcttccgggc tgtcatcatt aaactgtgca
     9541 atggcgatag ccttcgtcat ttcatgacca gcgtttatgc actggttaag tgtttccatg
     9601 agtttcattc tgaacatcct ttaatcattg ctttgcgttt ttttattaaa tcttgcaatt
     9661 tactgcaaag caacaacaaa atcgcaaagt catcaaaaaa ccgcaaagtt gtttaaaata
     9721 agagcaacac tacaaaagga gataagaaga gcacatacct cagtcactta ttatcactag
     9781 cgctcgccgc agccgtgtaa ccgagcatag cgagcgaact ggcgaggaag caaagaagaa
     9841 ctgttctgtc agatagctct tacgctcagc gcaagaagaa atatccaccg tgggaaaaac
     9901 tccaggtaga ggtacacacg cggatagcca attcagagta ataaactgtg ataatcaacc
     9961 ctcatcaatg atgacgaact aacccccgat atcaggtcac atgacgaagg gaaagagaag
    10021 gaaatcaact gtgacaaact gccctcaaat ttggcttcct taaaaattac agttcaaaaa
    10081 gtatgagaaa atccatgcag gctgaaggaa acagcaaaac tgtgacaaat taccctcagt
    10141 aggtcagaac aaatgtgacg aaccaccctc aaatctgtga cagataaccc tcagactatc
    10201 ctgtcgtcat ggaagtgata tcgcggaagg aaaatacgat atgagtcgtc tggcggcctt
    10261 tctttttctc aatgtatgag aggcgcattg gagttctgct gttgatctca ttaacacaga
    10321 cctgcaggaa gcggcggcgg aagtcaggca tacgctggta actttgaggc agctggtaac
    10381 gctctatgat ccagtcgatt ttcagagaga cgatgcctga gccatccggc ttacgatact
    10441 gacacaggga ttcgtataaa cgcatggcat acggattggt gatttctttt gtttcactaa
    10501 gccgaaactg cgtaaaccgg ttctgtaacc cgataaagaa gggaatgaga tatgggttga
    10561 tatgtacact gtaaagccct ctggatggac tgtgcgcacg tttgataaac caaggaaaag
    10621 attcatagcc tttttcatcg ccggcatcct cttcagggcg ataaaaaacc acttccttcc
    10681 ccgcgaaact cttcaatgcc tgccgtatat ccttactggc ttccgcagag gtcaatccga
    10741 atatttcagc atatttagca acatggatct cgcagatacc gtcatgttcc tgtagggtgc
    10801 catcagattt tctgatctgg tcaacgaaca gatacagcat acgtttttga tcccgggaga
    10861 gactatatgc cgcctcagtg aggtcgtttg actggacgat tcgcgggcta tttttacgtt
    10921 tcttgtgatt gataaccgct gtttccgcca tgacagatcc atgtgaagtg tgacaagttt
    10981 ttagattgtc acactaaata aaaaagagtc aataagcagg gataactttg tgaaaaaaca
    11041 gcttcttctg agggcaattt gtcacagggt taagggcaat ttgtcacaga caggactgtc
    11101 atttgagggt gatttgtcac actgaaaggg caatttgtca caacaccttc tctagaacca
    11161 gcatggataa aggcctacaa ggcgctctaa aaaagaagat ctaaaaacta taaaaaaaat
    11221 aattataaaa atatccccgt ggataagtgg ataaccccaa gggaagtttt ttcaggcatc
    11281 gtgtgtaagc agaatatata agtgctgttc cctggtgctt cctcgctcac tcgagggctt
    11341 cgccctgtcg ctcaactgcg gcgagcacta ctggctgtaa aaggacagac cacatcatgg
    11401 ttctgtgttc attaggttgt tctgtccatt gctgacataa tccgctccac ttcaacgtaa
    11461 caccgcacga agatttctat tgttcctgaa ggcatattca aatcgttttc gttaccgctt
    11521 gcaggcatca tgacagaaca ctacttccta taaacgctac acaggctcct gagattaata
    11581 atgcggatct ctacgataat gggagatttt cccgactgtt tcgttcgctt ctcagtggat
    11641 aacagccagc ttctctgttt aacagacaaa aacagcatat ccactcagtt ccacatttcc
    11701 atataaaggc caaggcattt attctcagga taattgtttc agcatcgcaa ccgcatcaga
    11761 ctccggcatc gcaaactgca cccggtgccg ggcagccaca tccagcgcaa aaaccttcgt
    11821 gtagacttcc gttgaactga tggacttatg tcccatcagg ctttgcagaa ctttcagcgg
    11881 tataccggca tacagcatgt gcatcgcata ggaatggcgg aacgtatgtg gtgtgaccgg
    11941 aacagagaac gtcacaccgt cagcagcagc ggcggcaacc gcctccccaa tccaggtcct
    12001 gaccgttctg tccgtcactt cccagatccg cgctttctct gtccttcctg tgcgacggtt
    12061 acgccgctcc atgagcttat cgcgaataaa tacctgtgac ggaagatcac ttcgcagaat
    12121 aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt tggcgaaaat
    12181 gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac
    12241 cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa
    12301 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
    12361 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
    12421 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
    12481 cccgcctgat gaatgctcat ccggagttcc gtatggcaat gaaagacggt gagctggtga
    12541 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
    12601 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
    12661 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
    12721 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
    12781 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
    12841 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
    12901 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag
    12961 gcagttattg gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga
    13021 tgaatggcag aaattcgatg ataagctgtc aaacatgaga attggtcgac ggcgcgccaa
    13081 agcttgcatg cctgcagccg cgtaacctgg caaaatcggt tacggttgag taataaatgg
    13141 atgccctgcg taagcggggc acatttcatt acctctttct ccgcacccga catagataat
    13201 aacttcgtat agtatacatt atacgaagtt atctagtaga cttaattaag gatcgatccg
    13261 gcgcgccaat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
    13321 agggcatcgg tcgagcttga cattgtagga ctatattgct ctaataaatt tgcggccgct
    13381 aatacgactc actataggga gaggatc
//
