Basic Vector Information
- Vector Name:
- pSV53hFasR
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9864 bp
- Type:
- Mammalian expression vector
- Replication origin:
- ori
- Source/Author:
- De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
- Promoter:
- SV40
pSV53hFasR vector Map
pSV53hFasR vector Sequence
LOCUS V002944 9864 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V002944
VERSION V002944
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 9864)
AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
Fonseca M, Vanhoucke M, Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9864)
AUTHORS De Schamphelaire W.
TITLE Direct Submission
JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM
REFERENCE 3 (bases 1 to 9864)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9864)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
9052, BELGIUM"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9864
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 82..439
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 453..974
/label="small t antigen"
/note="SV40 (simian virus 40) small t antigen"
intron 979..1044
/label="small t intron"
/note="SV40 (simian virus 40) small t antigen intron"
CDS 1174..1194
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 2948..3082
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
CDS complement(3106..4110)
/gene="FAS"
/label="Tumor necrosis factor receptor superfamily member
6"
/note="Tumor necrosis factor receptor superfamily member 6
from Homo sapiens. Accession#: P25445"
misc_feature complement(4111..4307)
/label="5UTR"
/note="5UTR"
misc_feature complement(4371..5308)
/label="intron"
/note="intron"
CDS complement(5315..5500)
/note="Agnoprotein from Simian virus 40. Accession#:
P03084"
/label="Agnoprotein"
promoter 5572..5901
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 5915..6436
/label="small t antigen"
/note="SV40 (simian virus 40) small t antigen"
CDS 6636..6656
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
misc_feature 7711..7851
/label="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(8037..8625)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8799..9656)
/label="AmpR"
/note="beta-lactamase"
promoter complement(9657..9761)
/label="AmpR promoter"
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