LOCUS       V002944                 9864 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002944
VERSION     V002944
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9864)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9864)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(3106..4110)
                     /gene="FAS"
                     /label="Tumor necrosis factor receptor superfamily member
                     6"
                     /note="Tumor necrosis factor receptor superfamily member 6
                     from Homo sapiens. Accession#: P25445"
     misc_feature    complement(4111..4307)
                     /label="5UTR"
                     /note="5UTR"
     misc_feature    complement(4371..5308)
                     /label="intron"
                     /note="intron"
     CDS             complement(5315..5500)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5572..5901
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5915..6436
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             6636..6656
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7711..7851
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8037..8625)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8799..9656)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9657..9761)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccgagctcc ccggggtcga ctctagacca agctttggat
     3121 ttcatttctg aagtttgaat tttctgagtc actagtaatg tccttgagga tgatagtctg
     3181 aattttctct gcaagagtac aaagattggc ttttttgaga tctttaatca atgtgtcata
     3241 cgcttctttc tttccatgaa gttgatgcca attacgaagc agttgaactt tctgttctgc
     3301 tgtgtcttgg acattgtcat tcttgatctc atctattttg gcttcattga caccattctt
     3361 tcgaacaaag cctttaactt gacttagtgt catgactcca gcaatagtgg tgatatattt
     3421 actcaagtca acatcagata aatttattgc cactgtttca ggatttaagg ttggagattc
     3481 atgagaacct tggttttcct ttctgtgctt tctgcatgtt ttctgtactt cctttctctt
     3541 cacccaaaca attagtggaa ttggcaaaag aagaagacaa agccacccca agttagatct
     3601 ggatccttcc tctttgcact tggtgttgct ggtgagtgtg cattccttga tgattccatg
     3661 ttcacatttg gtgcaagggt cacagtgttc acatacagta gagttacaaa aaaagtttgg
     3721 tttacatctg cacttggtat tctgggtccg ggtgcagttt atttccactt ctaagccatg
     3781 tccttcatca cacaatctac atcttctgca tttggaagaa aaatgggctt tgtctgtgta
     3841 ctccttccct tcttggcagg gcacgcagtc tggttcatcc ccattgactg tgcagtccct
     3901 agctttcctt tcacctggag gacagggctt atggcagaat tggccatcat gatgcaggcc
     3961 ttccaagttc tgagtctcaa ctgtagtaac agtcttcctc aattccaatc ccttggagtt
     4021 gatgtcagtc acttgggcat taacactttt ggacgataat ctagcaacag acgtaagaac
     4081 cagaggtagg agggtccaga tgcccagcat ggttgttgag caatcctccg aagtgaaaga
     4141 gcttccccaa ctccgtactg agcgggtcca ccaacccgcg ggagaagagg cagctccgcc
     4201 tgggcagcca gggctggcct cagggtgtgt tccgtgccag tgcccgcccc tgaggctcca
     4261 gccaagtcac tcgtaaaccg cttccctcac tccccagaag cgtcnnnctc gaggtcgagg
     4321 gatctctaga gatccggagc ttttagagca gaagtaacac ttccgtacag gcctagaagt
     4381 aaaggcaaca tccactgagg agcagttctt tgatttgcac caccaggagc ctcaaatttt
     4441 tcaataaatt cacctgactg cacattagga ctttggcttt gagcttccca cctctcagtt
     4501 acttgctgaa tactgtctgc ttcatcaata ttatcatagg tgtgcccaaa tgatatttgc
     4561 aacccttccc tgttggctac ttgtctcacc attgtaggcc taatgggaga caaagtagag
     4621 tagtaatctt gtaaagagtt ataccaatta acaggagcat taattactgt ccaagtagtt
     4681 tcctctaaaa accttgccaa actgtccctt aaatatcttt gggttcttct ttcaagctcc
     4741 tgtgaggtga gcctaggaat gtcattttgt attacacgcc aaaaagcttg agaaatggca
     4801 ttaaaaagtg ttggacccca atgtctgggg tcaagatact gaacactgtg aacaaaggtt
     4861 tgtactccag gaaataaaat atcatagtaa tcatctggcc tatacaaatc tacagccatt
     4921 cctggttgtt gatataaacc aacagtagaa actttgtgat cccagtcact aaaaaatcta
     4981 taccccactt gagcaacagc gctcacacca gtcacagttt gcagtaaagc tgcaaatcca
     5041 gctatagcag caggagcccc agatatcaca gcataggcct gtggagtgag gcctatagca
     5101 gcaattgcct cagaggttgt taggccttca acagtagcaa cagatgcaag ttgcacttca
     5161 attgcagcag cggcctctcc agcagcaatt tcagctactg aaaatccagt agcagcagca
     5221 gcttcagaca cagtagcaat taggtccccc aacagtgtta aagcagcacc catggacctg
     5281 aaataaaaga caaaaagact aaacttacca gttaactttc tggtttttca gttaaccttt
     5341 ctggtttttt gcgtttcccg tcaacagtat cttccccttc acaaaattgc agcaaaagct
     5401 ctaaaacaaa cacaaaaagg cgttgagctg tttttttact ttcagtccat gacctacgaa
     5461 ccttaacgga ggcctggcgt gacagccggc gcagcaccat ggcctgaaat aacctctgaa
     5521 agaggaactt ggttaggtac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     5581 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5641 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5701 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5761 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5821 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5881 ggaggcctag gcttttgcaa aaagctttgc aaagatggat aaagttttaa acagagagga
     5941 atctttgcag ctaatggacc ttctaggtct tgaaaggagt gcctggggga atattcctct
     6001 gatgagaaag gcatatttaa aaaaatgcaa ggagtttcat cctgataaag gaggagatga
     6061 agaaaaaatg aagaaaatga atactctgta caagaaaatg gaagatggag taaaatatgc
     6121 tcatcaacct gactttggag gcttctggga tgcaactgag gtatttgctt cttccttaaa
     6181 tcctggtgtt gatgcaatgt actgcaaaca atggcctgag tgtgcaaaga aaatgtctgc
     6241 taactgcata tgcttgctgt gcttactgag gatgaagcat gaaaatagaa aattatacag
     6301 gaaagatcca cttgtgtggg ttgattgcta ctgcttcgat tgctttagaa tgtggtttgg
     6361 acttgatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     6421 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     6481 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     6541 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
     6601 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
     6661 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
     6721 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
     6781 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
     6841 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
     6901 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
     6961 gtgccttgac tagagatcca ttttctgtta ttgaggaaag tttgccaggt gggttaaagg
     7021 agcatgattt taatccagaa gaagcagagg aaactaaaca agtgtcctgg aagcttgtaa
     7081 cagagtatgc aatggaaaca aaatgtgatg atgtgttgtt attgcttggg atgtacttgg
     7141 aatttcagta cagttttgaa atgtgtttaa aatgtattaa aaaagaacag cccagccact
     7201 ataagtacca tgaaaagcat tatgcaaatg ctgctatatt tgctgacagc aaaaaccaaa
     7261 aaaccatatg ccaacaggct gttgatactg ttttagctaa aaagcgggtt gatagcctac
     7321 aattaactag agaacaaatg ttaacaaaca gatttaatga tcttttggat aggatggata
     7381 taatgtttgg ttctacaggc tctgctgaca tagaagaatg gatggctgga gttgcttggc
     7441 tacactgttt gttgcccaaa atggattcag tggtgtatga ctttttaaaa tgcatggtgt
     7501 acaacattcc taaaaaaaga tactggctgt ttaaaggacc aattgatagt ggtaaaacta
     7561 cattagcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     7621 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     7681 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     7741 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     7801 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     7861 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7921 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7981 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8041 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8101 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8161 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8221 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8281 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8341 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8401 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8461 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8521 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8581 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8641 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8701 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8761 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8821 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8881 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8941 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9001 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9061 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
     9121 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9181 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9241 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9301 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9361 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     9421 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9481 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9541 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9601 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9661 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9721 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9781 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     9841 acgaggccct ttcgtcttca agaa
//
