pSV529-hTNF1 vector (V002949)

LOCUS       V002949                 9816 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002949
VERSION     V002949
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9816)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9816)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9816)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3085..3400)
                     /label="3UTR"
                     /note="3UTR"
     CDS             complement(3404..4102)
                     /gene="TNF"
                     /label="Tumor necrosis factor"
                     /note="Tumor necrosis factor from Homo sapiens. Accession#:
                     P01375"
     misc_feature    complement(4103..4280)
                     /label="5UTR"
                     /note="5UTR"
     misc_feature    complement(4323..5260)
                     /label="intron"
                     /note="intron"
     CDS             complement(5267..5452)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5524..5853
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5867..6388
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             6588..6608
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7663..7803
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7989..8577)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8751..9608)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9609..9713)
                     /label="AmpR promoter"