LOCUS       V002949                 9816 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002949
VERSION     V002949
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9816)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9816)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9816)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3085..3400)
                     /label="3UTR"
                     /note="3UTR"
     CDS             complement(3404..4102)
                     /gene="TNF"
                     /label="Tumor necrosis factor"
                     /note="Tumor necrosis factor from Homo sapiens. Accession#:
                     P01375"
     misc_feature    complement(4103..4280)
                     /label="5UTR"
                     /note="5UTR"
     misc_feature    complement(4323..5260)
                     /label="intron"
                     /note="intron"
     CDS             complement(5267..5452)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5524..5853
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5867..6388
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             6588..6608
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7663..7803
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7989..8577)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8751..9608)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9609..9713)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tctggcattc tggccagaac caaaggctcc ctggtctcca
     3121 gattccagat gtcagggatc aaagctgtag gccccagtga gttctggagg ccccagtttg
     3181 aattcttagt ggttgccagc aattcactgt gcaggccaca cattcctgaa tcccaggttt
     3241 cgaagtggtg gtcttgttgc ttaaagttct aagcttgggt tccgacccta agcccccaat
     3301 tctctttttg agccagaaga ggttgagggt gtctgaagga gggggtaata aagggattgg
     3361 ggcaggggag gcgtttggga aggttggatg ttcgtcctcc tcacagggca atgatcccaa
     3421 agtagacctg cccagactcg gcaaagtcga gatagtcggg ccgattgatc tcagcgctga
     3481 gtcggtcacc cttctccagc tggaagaccc ctcccagata gatgggctca taccagggct
     3541 tggcctcagc cccctctggg gtctccctct ggcaggggct cttgatggca gagaggaggt
     3601 tgaccttggt ctggtaggag acggcgatgc ggctgatggt gtgggtgagg agcacatggg
     3661 tggaggggca gccttggccc ttgaagagga cctgggagta gatgaggtac aggccctctg
     3721 atggcaccac cagctggtta tctctcagct ccacgccatt ggccaggagg gcattggccc
     3781 ggcggttcag ccactggagc tgcccctcag cttgagggtt tgctacaaca tgggctacag
     3841 gcttgtcact cggggttcga gaagatgatc tgactgcctg ggccagaggg ctgattagag
     3901 agaggtccct ggggaactct tccctctggg ggccgatcac tccaaagtgc agcaggcaga
     3961 agagcgtggt ggcgcctgcc acgatcagga aggagaagag gctgaggaac aagcaccgcc
     4021 tggagccctg gggcccccct gtcttcttgg ggagcgcctc ctcggccagc tccacgtccc
     4081 ggatcatgct ttcagtgctc atggtgtcct ttccagggga gagagggtgg agccgtgggt
     4141 cagtatgtga gaggaagaga acctgcctgg cagcttgtca ggggatgtgg cgtctgaggg
     4201 ttgttttcag ggggggtctg tagttgcttc tctccctctt agctggtcct cccccccccc
     4261 cccccccccc cccccccccc cagatccgga gcttttagag cagaagtaac acttccgtac
     4321 aggcctagaa gtaaaggcaa catccactga ggagcagttc tttgatttgc accaccagga
     4381 gcctcaaatt tttcaataaa ttcacctgac tgcacattag gactttggct ttgagcttcc
     4441 cacctctcag ttacttgctg aatactgtct gcttcatcaa tattatcata ggtgtgccca
     4501 aatgatattt gcaacccttc cctgttggct acttgtctca ccattgtagg cctaatggga
     4561 gacaaagtag agtagtaatc ttgtaaagag ttataccaat taacaggagc attaattact
     4621 gtccaagtag tttcctctaa aaaccttgcc aaactgtccc ttaaatatct ttgggttctt
     4681 ctttcaagct cctgtgaggt gagcctagga atgtcatttt gtattacacg ccaaaaagct
     4741 tgagaaatgg cattaaaaag tgttggaccc caatgtctgg ggtcaagata ctgaacactg
     4801 tgaacaaagg tttgtactcc aggaaataaa atatcatagt aatcatctgg cctatacaaa
     4861 tctacagcca ttcctggttg ttgatataaa ccaacagtag aaactttgtg atcccagtca
     4921 ctaaaaaatc tataccccac ttgagcaaca gcgctcacac cagtcacagt ttgcagtaaa
     4981 gctgcaaatc cagctatagc agcaggagcc ccagatatca cagcataggc ctgtggagtg
     5041 aggcctatag cagcaattgc ctcagaggtt gttaggcctt caacagtagc aacagatgca
     5101 agttgcactt caattgcagc agcggcctct ccagcagcaa tttcagctac tgaaaatcca
     5161 gtagcagcag cagcttcaga cacagtagca attaggtccc ccaacagtgt taaagcagca
     5221 cccatggacc tgaaataaaa gacaaaaaga ctaaacttac cagttaactt tctggttttt
     5281 cagttaacct ttctggtttt ttgcgtttcc cgtcaacagt atcttcccct tcacaaaatt
     5341 gcagcaaaag ctctaaaaca aacacaaaaa ggcgttgagc tgttttttta ctttcagtcc
     5401 atgacctacg aaccttaacg gaggcctggc gtgacagccg gcgcagcacc atggcctgaa
     5461 ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg
     5521 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5581 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     5641 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     5701 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5761 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     5821 ggaggctttt ttggaggcct aggcttttgc aaaaagcttt gcaaagatgg ataaagtttt
     5881 aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgcctgggg
     5941 gaatattcct ctgatgagaa aggcatattt aaaaaaatgc aaggagtttc atcctgataa
     6001 aggaggagat gaagaaaaaa tgaagaaaat gaatactctg tacaagaaaa tggaagatgg
     6061 agtaaaatat gctcatcaac ctgactttgg aggcttctgg gatgcaactg aggtatttgc
     6121 ttcttcctta aatcctggtg ttgatgcaat gtactgcaaa caatggcctg agtgtgcaaa
     6181 gaaaatgtct gctaactgca tatgcttgct gtgcttactg aggatgaagc atgaaaatag
     6241 aaaattatac aggaaagatc cacttgtgtg ggttgattgc tactgcttcg attgctttag
     6301 aatgtggttt ggacttgatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     6361 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     6421 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     6481 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     6541 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     6601 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     6661 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     6721 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
     6781 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
     6841 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
     6901 atttgatgta tagtgccttg actagagatc cattttctgt tattgaggaa agtttgccag
     6961 gtgggttaaa ggagcatgat tttaatccag aagaagcaga ggaaactaaa caagtgtcct
     7021 ggaagcttgt aacagagtat gcaatggaaa caaaatgtga tgatgtgttg ttattgcttg
     7081 ggatgtactt ggaatttcag tacagttttg aaatgtgttt aaaatgtatt aaaaaagaac
     7141 agcccagcca ctataagtac catgaaaagc attatgcaaa tgctgctata tttgctgaca
     7201 gcaaaaacca aaaaaccata tgccaacagg ctgttgatac tgttttagct aaaaagcggg
     7261 ttgatagcct acaattaact agagaacaaa tgttaacaaa cagatttaat gatcttttgg
     7321 ataggatgga tataatgttt ggttctacag gctctgctga catagaagaa tggatggctg
     7381 gagttgcttg gctacactgt ttgttgccca aaatggattc agtggtgtat gactttttaa
     7441 aatgcatggt gtacaacatt cctaaaaaaa gatactggct gtttaaagga ccaattgata
     7501 gtggtaaaac tacattagca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     7561 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     7621 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     7681 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     7741 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     7801 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7861 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7921 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7981 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8041 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8101 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8161 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8221 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8281 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8341 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8401 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     8461 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8521 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8581 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8641 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8701 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8761 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8821 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8881 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8941 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9001 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9061 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9121 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9181 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9241 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9301 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9361 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9421 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9481 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9541 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9601 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9661 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9721 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     9781 aataggcgta tcacgaggcc ctttcgtctt caagaa
//
