pSV51E6Hy3f22 vector (V002950)

LOCUS       V002950                10612 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002950
VERSION     V002950
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10612)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10612)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10612)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10612)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3105..3200)
                     /label="fragment of CH2 domain of mIghg2b"
                     /note="fragment of CH2 domain of mIghg2b"
     misc_feature    complement(3201..3318)
                     /label="intron of mIghg2b"
                     /note="intron of mIghg2b"
     misc_feature    complement(3319..3363)
                     /label="H4 domain of mIghg2b"
                     /note="H4 domain of mIghg2b"
     misc_feature    complement(3364..3506)
                     /label="intron of mIghg2b"
                     /note="intron of mIghg2b"
     misc_feature    complement(3507..3551)
                     /label="H3 domain of mIghg2b"
                     /note="H3 domain of mIghg2b"
     misc_feature    complement(3552..3694)
                     /label="intron of mIghg2b"
                     /note="intron of mIghg2b"
     misc_feature    complement(3695..3739)
                     /label="H2 domain of mIghg2b"
                     /note="H2 domain of mIghg2b"
     misc_feature    complement(3740..3882)
                     /label="intron of mIghg2b"
                     /note="intron of mIghg2b"
     misc_feature    complement(3883..3933)
                     /label="H1 domain of mIghg2b"
                     /note="H1 domain of mIghg2b"
     misc_feature    complement(3934..4324)
                     /label="intron of mIghg2b"
                     /note="intron of mIghg2b"
     misc_feature    complement(4325..4615)
                     /label="CH1 domain of mIghg2b"
                     /note="CH1 domain of mIghg2b"
     misc_feature    complement(4616..4975)
                     /label="VH domain of mIghg2b"
                     /note="VH domain of mIghg2b"
     misc_feature    complement(4976..5032)
                     /label="L domain of mIghg2b"
                     /note="L domain of mIghg2b"
     misc_feature    complement(5119..6056)
                     /label="intron of mIghg2b"
                     /note="intron of mIghg2b"
     CDS             complement(6063..6248)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        6320..6649
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             6663..7184
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             7384..7404
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    8459..8599
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8785..9373)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9547..10404)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10405..10509)
                     /label="AmpR promoter"