LOCUS       V002950                10612 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002950
VERSION     V002950
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10612)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10612)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10612)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10612)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3105..3200)
                     /label="fragment of CH2 domain of mIghg2b"
                     /note="fragment of CH2 domain of mIghg2b"
     misc_feature    complement(3201..3318)
                     /label="intron of mIghg2b"
                     /note="intron of mIghg2b"
     misc_feature    complement(3319..3363)
                     /label="H4 domain of mIghg2b"
                     /note="H4 domain of mIghg2b"
     misc_feature    complement(3364..3506)
                     /label="intron of mIghg2b"
                     /note="intron of mIghg2b"
     misc_feature    complement(3507..3551)
                     /label="H3 domain of mIghg2b"
                     /note="H3 domain of mIghg2b"
     misc_feature    complement(3552..3694)
                     /label="intron of mIghg2b"
                     /note="intron of mIghg2b"
     misc_feature    complement(3695..3739)
                     /label="H2 domain of mIghg2b"
                     /note="H2 domain of mIghg2b"
     misc_feature    complement(3740..3882)
                     /label="intron of mIghg2b"
                     /note="intron of mIghg2b"
     misc_feature    complement(3883..3933)
                     /label="H1 domain of mIghg2b"
                     /note="H1 domain of mIghg2b"
     misc_feature    complement(3934..4324)
                     /label="intron of mIghg2b"
                     /note="intron of mIghg2b"
     misc_feature    complement(4325..4615)
                     /label="CH1 domain of mIghg2b"
                     /note="CH1 domain of mIghg2b"
     misc_feature    complement(4616..4975)
                     /label="VH domain of mIghg2b"
                     /note="VH domain of mIghg2b"
     misc_feature    complement(4976..5032)
                     /label="L domain of mIghg2b"
                     /note="L domain of mIghg2b"
     misc_feature    complement(5119..6056)
                     /label="intron of mIghg2b"
                     /note="intron of mIghg2b"
     CDS             complement(6063..6248)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        6320..6649
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             6663..7184
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             7384..7404
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    8459..8599
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8785..9373)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9547..10404)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10405..10509)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
       61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
      121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
      481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
      541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
      601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
      661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
      721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
      781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
      841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
      901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
      961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
     1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
     1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
     1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
     1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
     1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
     1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
     1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
     1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
     2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
     2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
     2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
     2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
     2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
     2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
     2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
     2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
     2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
     2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
     2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
     2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
     2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
     2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3061 tgtatcttat catgtctgga tccggctcta gagggtcgac gagcttagtt aactaagtca
     3121 ggggtccggg aaatcataag ggtatccttg ggttttgggg ggaagaggaa gactgacggt
     3181 cctcccagga gttcaggtgc tgaggaagag atggaggcag atgtgtcagc acccgactgg
     3241 gacctgtccc tggatgcagg ccactctagg gcacctgtcc tgccttgagc tggagggcga
     3301 ggcctgggct ggcttacctg ggcaccttgg gcacggggga ggtgtgtcac aagatttggg
     3361 ctctgcagag agaagattgg gagttacggg gatctgggat ggaggtggat gcgtcagcac
     3421 cctgctgggg cctgtccctg gactcaggcc actctagggt acttgtcccg ccttgagctg
     3481 gagggcgagg cctgggctgg cttacctggg caccgtgggc atgggggagg tgtgtcacaa
     3541 gatttgggct ctgcagagag aagattggga gttacgggga tctgggatgg aggtggacgc
     3601 gtcagcaccc tgctggggcc tgtccctgga ctcaggccac tctagggctc ttgtcctgcc
     3661 ttgagctgga gggtgaggcc tgggctggct tacctgggca ccgtgggcac gggggaggtg
     3721 tgtcacaaga tttgggctct gcagagagaa gattgggagt tacggggatc tgggatggag
     3781 gtggatgcgt cagcaccctg ctggggcctg tccctggact caggccactc tagggctctt
     3841 gtcccgcctt gagctggagg gcgagtcctg ggctggctta cctgggcacc gtgggcatgt
     3901 gtgagttgtg tcaccaagtg gggttttgag ctctgcagag agaagattgg gagttactca
     3961 gatctgggaa gagagaaggt gtctgagctg agtgagtaga gagtttggcc tttggggtgg
     4021 gcttaggtca ggggcagggt cctcctggat atggcttttg gcagctctga gcgcagcacc
     4081 tgcccctgtg tgtgaagggc ctggggtagg ggcatccagc ctgtgcctgc ccggagcctg
     4141 gtggaaaaag ccagaagacc ctctccctga gcatgagtgg ggcgggcaga ggctccgggt
     4201 gaggagtcag acggggcctg ccttgctgcc ctgggctggg actgcacagc cgggatgcgt
     4261 ccaggcagga gggctgagcc tggcttccag cagacaccct ccctccctgc gctggcctct
     4321 caccaactct cttgtccacc ttggtgttgc tgggcttgtg attcacgttg caggtgtagg
     4381 tctgggtgcc caagctgctg gagggcacgg tcaccacgct gctgagggag tagagtccag
     4441 actgcaggag agctgggaag gtgtgcacac tgctggacag ggatccggag ttccaagtca
     4501 cagtcactga ctcagggaag tagcccttga ccaggcatcc cagagtcacg gaggaaccag
     4561 ttgtatctcc acacccaggg gccagtggat agactgatgg gggtgttgtt ttggctgcag
     4621 aggcagtgac cagagtccct tggccccagt aaccaaaagg gagtagcccg acctgtctag
     4681 gtccggcaca gaaatagacg gcagagtcct cagatgtcag gctgctgagg tgcaagtagg
     4741 ctgtgctgga ggatttgtct acagtcagtg tggccttgcc cttaaacttc tcattgaagt
     4801 acgaatttcc acttccagga taaatttctc caatccactc aaggccctgt ccagttctct
     4861 gcttcaccca acttatacca taacttgtga gggtgtatcc agaggccttg caggacagct
     4921 tcactgaggc cccaggcctc gccagctcag ctccagactg ctgcagctga acctgggatt
     4981 ggacacctgc agttcctgac aggatgaaga gaaagatcca gatccattcc atgctagagc
     5041 caagctcgtc gaccctctag aggctgcagc cgaccccgga tccggagctt ttagagcaga
     5101 agtaacactt ccgtacaggc ctagaagtaa aggcaacatc cactgaggag cagttctttg
     5161 atttgcacca ccaggagcct caaatttttc aataaattca cctgactgca cattaggact
     5221 ttggctttga gcttcccacc tctcagttac ttgctgaata ctgtctgctt catcaatatt
     5281 atcataggtg tgcccaaatg atatttgcaa cccttccctg ttggctactt gtctcaccat
     5341 tgtaggccta atgggagaca aagtagagta gtaatcttgt aaagagttat accaattaac
     5401 aggagcatta attactgtcc aagtagtttc ctctaaaaac cttgccaaac tgtcccttaa
     5461 atatctttgg gttcttcttt caagctcctg tgaggtgagc ctaggaatgt cattttgtat
     5521 tacacgccaa aaagcttgag aaatggcatt aaaaagtgtt ggaccccaat gtctggggtc
     5581 aagatactga acactgtgaa caaaggtttg tactccagga aataaaatat catagtaatc
     5641 atctggccta tacaaatcta cagccattcc tggttgttga tataaaccaa cagtagaaac
     5701 tttgtgatcc cagtcactaa aaaatctata ccccacttga gcaacagcgc tcacaccagt
     5761 cacagtttgc agtaaagctg caaatccagc tatagcagca ggagccccag atatcacagc
     5821 ataggcctgt ggagtgaggc ctatagcagc aattgcctca gaggttgtta ggccttcaac
     5881 agtagcaaca gatgcaagtt gcacttcaat tgcagcagcg gcctctccag cagcaatttc
     5941 agctactgaa aatccagtag cagcagcagc ttcagacaca gtagcaatta ggtcccccaa
     6001 cagtgttaaa gcagcaccca tggacctgaa ataaaagaca aaaagactaa acttaccagt
     6061 taactttctg gtttttcagt taacctttct ggttttttgc gtttcccgtc aacagtatct
     6121 tccccttcac aaaattgcag caaaagctct aaaacaaaca caaaaaggcg ttgagctgtt
     6181 tttttacttt cagtccatga cctacgaacc ttaacggagg cctggcgtga cagccggcgc
     6241 agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg
     6301 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6361 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6421 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6481 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6541 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6601 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttgcaa
     6661 agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg
     6721 aaaggagtgc ctgggggaat attcctctga tgagaaaggc atatttaaaa aaatgcaagg
     6781 agtttcatcc tgataaagga ggagatgaag aaaaaatgaa gaaaatgaat actctgtaca
     6841 agaaaatgga agatggagta aaatatgctc atcaacctga ctttggaggc ttctgggatg
     6901 caactgaggt atttgcttct tccttaaatc ctggtgttga tgcaatgtac tgcaaacaat
     6961 ggcctgagtg tgcaaagaaa atgtctgcta actgcatatg cttgctgtgc ttactgagga
     7021 tgaagcatga aaatagaaaa ttatacagga aagatccact tgtgtgggtt gattgctact
     7081 gcttcgattg ctttagaatg tggtttggac ttgatctttg tgaaggaacc ttacttctgt
     7141 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     7201 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
     7261 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
     7321 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
     7381 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
     7441 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
     7501 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
     7561 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
     7621 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
     7681 ggggttaata aggaatattt gatgtatagt gccttgacta gagatccatt ttctgttatt
     7741 gaggaaagtt tgccaggtgg gttaaaggag catgatttta atccagaaga agcagaggaa
     7801 actaaacaag tgtcctggaa gcttgtaaca gagtatgcaa tggaaacaaa atgtgatgat
     7861 gtgttgttat tgcttgggat gtacttggaa tttcagtaca gttttgaaat gtgtttaaaa
     7921 tgtattaaaa aagaacagcc cagccactat aagtaccatg aaaagcatta tgcaaatgct
     7981 gctatatttg ctgacagcaa aaaccaaaaa accatatgcc aacaggctgt tgatactgtt
     8041 ttagctaaaa agcgggttga tagcctacaa ttaactagag aacaaatgtt aacaaacaga
     8101 tttaatgatc ttttggatag gatggatata atgtttggtt ctacaggctc tgctgacata
     8161 gaagaatgga tggctggagt tgcttggcta cactgtttgt tgcccaaaat ggattcagtg
     8221 gtgtatgact ttttaaaatg catggtgtac aacattccta aaaaaagata ctggctgttt
     8281 aaaggaccaa ttgatagtgg taaaactaca ttagcagctg cctcgcgcgt ttcggtgatg
     8341 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     8401 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     8461 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     8521 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     8581 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8641 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8701 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8761 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8821 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8881 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8941 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9001 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9061 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9121 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9181 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     9241 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9301 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9361 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9421 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9481 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9541 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9601 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9661 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9721 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9781 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9841 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9901 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9961 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    10021 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10081 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10141 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
    10201 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10261 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10321 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10381 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10441 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10501 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    10561 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aa
//
