pSUN119 vector (V003015)

LOCUS       40924_41430        9816 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle reporter vector pSUN119, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   Argueta C, Yuksek K, Summers M.
  TITLE     Construction and use of GFP reporter vectors for analysis of 
            cell-type-specific gene expression in Nostoc punctiforme
  JOURNAL   J. Microbiol. Methods 59 (2), 181-188 (2004)
  PUBMED    15369854
REFERENCE   2  (bases 1 to 9816)
  AUTHORS   Argueta C, Yuksek K, Summers ML.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2004) Biology, CSU Northridge, 18111 Nordhoff St.,
            Northridge, CA 91330-8303, USA
REFERENCE   3  (bases 1 to 9816)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9816)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods"; date: "2004"; volume: "59"; issue: "2"; pages: 
            "181-188"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-MAY-2004) Biology, CSU Northridge, 18111 Nordhoff St., 
            Northridge, CA 91330-8303, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(221..934)
                     /codon_start=1
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    997..1033
                     /label=multiple cloning site
                     /note="multiple cloning site"
     terminator      complement(1215..1262)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1311..1327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1335..1351)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1359..1389)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1404..1425)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1713..2301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2803..3594)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      3958..9816
                     /label=pDC1 cyanobacterial origin of replication
                     /note="pDC1 cyanobacterial origin of replication"