LOCUS       40924_41430        9816 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle reporter vector pSUN119, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   Argueta C, Yuksek K, Summers M.
  TITLE     Construction and use of GFP reporter vectors for analysis of 
            cell-type-specific gene expression in Nostoc punctiforme
  JOURNAL   J. Microbiol. Methods 59 (2), 181-188 (2004)
  PUBMED    15369854
REFERENCE   2  (bases 1 to 9816)
  AUTHORS   Argueta C, Yuksek K, Summers ML.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2004) Biology, CSU Northridge, 18111 Nordhoff St.,
            Northridge, CA 91330-8303, USA
REFERENCE   3  (bases 1 to 9816)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9816)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods"; date: "2004"; volume: "59"; issue: "2"; pages: 
            "181-188"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-MAY-2004) Biology, CSU Northridge, 18111 Nordhoff St., 
            Northridge, CA 91330-8303, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(221..934)
                     /codon_start=1
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    997..1033
                     /label=multiple cloning site
                     /note="multiple cloning site"
     terminator      complement(1215..1262)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1311..1327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1335..1351)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1359..1389)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1404..1425)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1713..2301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2803..3594)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      3958..9816
                     /label=pDC1 cyanobacterial origin of replication
                     /note="pDC1 cyanobacterial origin of replication"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggccag tgaattcgta tttgtagagc tcatccatgc
      241 catgtgtaat cccagcagca gttacaaact caagaaggac catgtggtca cgcttttcgt
      301 tgggatcttt cgaaagggca gattgtgtcg acaggtaatg gttgtctggt aaaaggacag
      361 ggccatcgcc aattggagta ttttgttgat aatggtctgc tagttgaacg gatccatctt
      421 caatgttgtg gcgaattttg aagttagctt tgattccatt cttttgtttg tctgccgtga
      481 tgtatacatt gtgtgagtta tagttgtact cgagtttgtg tccgagaatg tttccatctt
      541 ctttaaaatc aatacctttt aactcgatac gattaacaag ggtatcacct tcaaacttga
      601 cttcagcacg cgtcttgtag ttcccgtcat ctttgaaaga tatagtgcgt tcctgtacat
      661 aaccttcggg catggcactc ttgaaaaagt catgccgttt catatgatct gggtatcttg
      721 aaaagcattg aacaccataa gagaaagtag tgacaagtgt tggccatgga acaggtagtt
      781 ttccagtagt gcaaataaat ttaagggtaa gttttccgta tgttgcatca ccttcaccct
      841 ctccactgac agaaaatttg tgcccattaa catcaccatc taattcaaca agaattggga
      901 caactccagt gaaaagttct tctcctttac tcattatgag tctccagttt gtttcagtta
      961 aaacgtagta gtgttggtaa attactcgag gaattcggta ccccgggttc gaaatcgata
     1021 agcttggctg caggtcgact ctagcgctat atgcgttgat gcaatttcta tgcgcacccg
     1081 ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg
     1141 gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggata tccggatata
     1201 gttcctcctt tcagcaaaaa acccctcaag acccgtttag aggccccaag gggttatgct
     1261 agttattgct cagcggtggc agcagcgcat gcaagcttgg cgtaatcatg gtcatagctg
     1321 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     1381 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1441 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1501 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     1561 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1621 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1681 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1741 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1801 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1861 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1921 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1981 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2041 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2101 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     2161 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2221 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2281 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2341 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2401 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2461 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     2521 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     2581 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggg tgggcgaaga
     2641 actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc
     2701 cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg
     2761 gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     2821 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     2881 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     2941 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     3001 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     3061 catccgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     3121 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     3181 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     3241 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     3301 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     3361 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttggagttc
     3421 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     3481 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     3541 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     3601 cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg
     3661 caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
     3721 tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag
     3781 cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca
     3841 gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgttccg
     3901 cttcctttag cagcccttgc gccctgagtg cttgcggcag cgtgaagcta gcttaaacgc
     3961 tcaaattgca gctttctagc cacgctcctg taaaaaatat atttctgaac agttaacctg
     4021 tcagaagtac attctctact tttaacggtc acgcgtctcg tgccgattag tatacttatt
     4081 gccgatgcat ttaccaaaat ttgcaaccgg agaagttcac ttgtccagta aaaacttcca
     4141 aaaatttacc ggacaacctt atgttatccg ccagcaaatt ttgacgcaca gtacgccagt
     4201 tttggctgaa agtttcaact gttgcgaaaa gtgcctagtg aactcgttgt acaaaaaaat
     4261 tttcgcgttt ggcatcaagc cacgcactct taaggttttg acaaaatatg tcattccaag
     4321 cttctgcaat ggaatttaaa gttaatcccc atccgcgata tttcggcact agggaagcag
     4381 catgatccat gcattggcgc agactgccag agcatagctc atcacatgtc cagactacct
     4441 ggttttcttc atccaactca tgttctacca gcatcttcaa aacccacatc ttaccccctc
     4501 aaaaaaattc tcaaaaatgt cccgtcactt cctggggctt gtgatgattg ctttgcaatc
     4561 gtctgcctgc gggacggctc gattttcgtg aaactggcac tgtgccagtt tacaacgcca
     4621 cttccgccgc tttgcgcgca tggttgttag cttgaattgc tcgcggtaaa tgttgcagcg
     4681 agccatccag gttttaccct tcatttggaa gaaacgccca gacagttccc cgctggcagc
     4741 tggtaagtgt tcggctgctg ctttctggct cctggcagga tgccatcaga aaacgcgatc
     4801 gccccttcac ccaatgactg atacccaatg actgataccc aatgtctgac tgagtgccct
     4861 cgctactcca agcccagcac tcagacgctt cattgcttac agcccggtca agttgagcgt
     4921 attcatactt cgcttggcac agttgccttt tctccatgcc gagcgttcgc tgatgccgcc
     4981 gcactgtgcg cgtgtggatg cccaatttac agctgaccgc attttgagaa gtcccgtaat
     5041 gaatgaagct tttagaaacg aacacccgcg acggactgac atgaagaaca aaaggtactt
     5101 cccccgctcc caatttaaac gaggattgcc cctcatcccc caacagttcg tttggatgtg
     5161 gcgctccgta agacttgcga tactccggtt tcagtttgcg gttagccgcg tatctacttc
     5221 tttgttgcaa acgctgtgtg actatgcccg tggtcagcgc tcgaatgttg gcgttgacct
     5281 cccataacgg acattctgct actacccccc aatcttttaa atttaaatgc caagccacct
     5341 tagtcagtcc gcccaggtaa accgtgagca gtccaattct taccttgtat cccgacaaaa
     5401 atgcccctac tgcttttccc tggtcaagcc atctgtacac agtggcttga ctaactccca
     5461 atatctcctc taccagcacg agcggaattt ctttaacccg tccgctccca tcatcaaaac
     5521 cgcgcaacaa ataccacagc cgcgcccaag gttgacggct aattccactg tgtaaccgca
     5581 ccatgaacgg ctgcaaaatt atctcggctt tgactagtat caaaaccaac gggagtggta
     5641 catttgaact taaggctgaa cgatcctcct gtgaagtcag ggggcaatgg ttcagcccct
     5701 caacttgggt agctgcgcga ttttgattgg ccactggttt tcactcaaac ctggttcaat
     5761 gtgccaatcg cttcactaaa tttaagtttt cgcttccgct cgactgaata ctgtgctgat
     5821 cctcgaaggg tggtaatatg tttgaagttc attgcttctg ggtgattaag gaatgattgc
     5881 ccagtagttt ttccaaatct tcaccctatc tctacactgt ttaccgatcc acgcagttgt
     5941 ataccaaccc caaaacttga taaattcaag ttaacttttg ttattgtctg gtttttggat
     6001 tacggacttg atgggcctgg tgaacgactg ctgcatggaa ctctgtaaac atgtgtagac
     6061 gactgggtaa tcaatttctc cactaccgga ctttttctta tataagtttt cttgtgtaag
     6121 atttgcactt ttagaagtat gcgtatccta ttaggaaaac tcacagtttt aagaaaaact
     6181 gtatttagca tacatcggtt agagccgtgt atcaattgac aacatcctat acaggatcta
     6241 ggttttgcac gtgccataag gtgacgaaat atttatttcg tcacataaag cttgaacagt
     6301 aatcactgca agcagttgaa attctacccg tgtagtatgg gtatagaatt ttcctttttc
     6361 tgtttttttg tagtcaaaat ttaggcaaaa catgctactc atttgctact caaaatatgt
     6421 ttgaaaccat tgattaatcg tttatgccaa catttcaccc caaaatacac gaaatcttgt
     6481 aactattgct atgactagct ttcacccgtt ggtgtaatga ttctgtagaa tgttgtaccc
     6541 ccttactggt aaacccttgc tgtgactagc tttcatcaat gaccaatgga tttcaagtcc
     6601 gcgtgtcctt agttatcccc gctacatcta tctgccattc tgcttttcaa gcattaaaag
     6661 cattgtttcc aattcttccc aatgcggttg tgaaagagtt tggtagcact gaagcaacag
     6721 ttcggaatgc tccggcgcat catcagaagc tatgcttaac agcttgtggt attgtttaag
     6781 ctggttaaat tcatccaagg cacgggtaat caaaaccttt gcagattcgt ttctagtctg
     6841 tgacatcatg ttttgcatcc ccgattacat tggttctact tggcactcat cgacccattc
     6901 cacttcacgg tccgtctact tcaaaccagg gatgctcttc tggcaaggac accgcatatt
     6961 gccaaaaagc accagtccaa aaaagcccaa tcacctcaac aggatgcaga atttcccccc
     7021 tggggactgt catcctatgc agcacggttt gcccgatatg atatccaggt ttactaaaag
     7081 cctttgtaat ccgcaaatgc caaaaactca gcgcgttgca tcgcagcagc ccgaaacggg
     7141 cgcgtttaac gctaccattg cttattgcct ccttagtggg ctaacaactt ttaaggggga
     7201 agtcgtgccg gtgggggaga tgtggtaatt tctcctgccg gggcgcattt aaggcctgac
     7261 ttcatttgtc gcatcaggta tcttgaatta ttgaaccaga tttgagcgca aaaagcgatc
     7321 gcgctgcggg gcgattactg catgttctag cttgagcagt atttattctg ctagaactct
     7381 tgagctactt tattcgggac gggagatcgt ttaaccatcc tatgtgggta gtgattttca
     7441 agggagcgag acgacgctac gatttactcg ctacaacctt gtttactatc gtctactttt
     7501 tgtctacaaa cagtctaagg tagtaaatca tgtattgtca acattgtcta caattttgtg
     7561 gacaatctct ctaggttaac ctagtatgta gactaggtgt atacaaatag tctacaaatg
     7621 cgtaacaata taatttcatt tcgctttagt gacgctgaat tagaagcgtt gcaagcattc
     7681 caaactccag aagataaatc ccctagccaa atagccgcaa gattagtaag gggaataatt
     7741 gcagggatgg tagaaccgtc tacaccgtct acacctgtag acatacagga attagttaag
     7801 cagcaagtcg agcaggctat caccgactcg caactgttga aagatatgat tgcgctcaat
     7861 acctctcacc taacaaaagg catcgacaaa attaagcagg aggtcgatga acgtttggag
     7921 gaactgcggg gaaaatcgaa agcccggtag cagagaagga tactagcgct accgggcaag
     7981 taatgaataa tagcgactta atcttagtcc gtgatcgcgt ccttgccaag ctgaaaatgg
     8041 gtaagcaatc aaccgccggg aaggcaatcg acgcatttat caaagaactg cacaaaactc
     8101 cgaccgcacc ggaggaacaa aagccgtcta cctgggaagc ccgcgctgac aacttcctca
     8161 aggaggttgc aggttgcagg caatggctaa ccaccgaatc aaaatgcgcc tcatgggtac
     8221 taaggaagat ttggagcgct ggctgtggtt tgtgggcaag atgcaagagc ggggactggc
     8281 aagcatcatt gagaaaagtt cgccttacaa aaacagaggc gaatcgctgc aacaccgcgt
     8341 ctacttggaa gttgacttgc tgttggatgc cccagaagat gagattaagc ctttataacc
     8401 ccagtacagt gccacacatg gacggtttca acctgtctac agtccgtcta caccacaaca
     8461 acacacgacg atcggtgcgg ttacagtgca acaatagacg gtgacaagtg cggttgcagt
     8521 gcaacaatag acggtgacaa gtgcggttgc agtgcaacaa tagagcgtct acacccaaac
     8581 cagttaaaaa attgcctgta gacggctaaa aaccgctttt gaagagactt tcagcaactg
     8641 tagacaaatt tttgggggag catttttaac agaaataccc caaaagttag ccatttttct
     8701 taacagaatg ctgatttttg tagactcgtt cggcttaaca aaatgctcaa aaacatagca
     8761 gccgggtgcg aacgtggttg caattctcca atgttctagc tttaaatatc agaatgtaac
     8821 tagaactctg tggaaccatg cccaaaaaga gatgcggact cggctttgac tgcgggtcga
     8881 tgatgttaca acctgggata gaccccggcg actgcgaaaa ttacgccacc tgtggaggag
     8941 ccacccaact caccccagaa gaagagtttg aattgatacg ttggcgggaa attcaaaacc
     9001 gggaaaggca agaggagtgg gagcgccagc gcgaagcaat tcgggtcaac cgccaccaag
     9061 ctgcattgat gatgctgatg ggtcgcgctt gcccccaaac gcccgaatca ttgggtgttg
     9121 tggatttgtt agcccaagtt gcaaccttag ttgaggaagt cagatcgcaa ctgccccagt
     9181 ttgaagggca gtacatcgca cctgaaaagt gcgaagccca cacttacaac gtcaaacgac
     9241 ccaaaggtgt atattgctac aataagctga ccgctgccga ggcaattttt gagccgagcg
     9301 agaaaagcga taaagtccga gtcatccacc tcagccacga cggcgacccc cgcgactcag
     9361 aggcgcggct gggcatcgaa cgccgaaacg ccttgactca agccaggact cagatcaacg
     9421 ttgcccaagt tgcactcgct caagccttgg agttgctgca atgttctagc agaataaata
     9481 atcagcaagc tagaacactc gcgcactcgc ccccatccag tgacgaaaca ccgtgaaagg
     9541 cgatcgcccc aggtgcatca aaatttgcaa agtgttttat tgcaaatctt gatgcagcca
     9601 cgcactgcat cactcaccag cttccagctt ccgacttcca cttacttcaa ctagctactc
     9661 taactggaag ttgaagttgt aagttacctt gcgggtctaa ttttcctatt ctgattcgct
     9721 agattcttct tttagacttt catcgtatgt ttgataaacc tggatgcaac gtcctggttt
     9781 acgctcaaag ctttccgccg atataacaaa agaatt
//
