Basic Vector Information
pSC101 ori is a low-copy replication origin that requires the Rep101 protein, which is a temperature-sensitive protein. When bacteria containing a plasmid with this ori is cultured at 37℃, the plasmid will be lost.
- Vector Name:
- pSTNSK
- Antibiotic Resistance:
- Kanamycin
- Length:
- 10008 bp
- Type:
- Tn7 transposase expression vector
- Replication origin:
- pSC101 ori
- Source/Author:
- Crepin S, Harel J, Dozois CM.
- Copy Number:
- Low Copy
- Growth Temperature:
- 30℃
pSTNSK vector Map
pSTNSK vector Sequence
LOCUS V003024 10008 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V003024
VERSION V003024
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 10008)
AUTHORS Crepin S, Harel J, Dozois CM.
TITLE Chromosomal complementation using Tn7 transposon vectors in
Enterobacteriaceae
JOURNAL Appl. Environ. Microbiol. 78 (17), 6001-6008 (2012)
PUBMED 22706059
REFERENCE 2 (bases 1 to 10008)
AUTHORS Crepin S, Harel J, Dozois CM.
TITLE Direct Submission
JOURNAL Submitted (23-JAN-2012) Microbiology and Biothechnology,
INRS-Institut Armand-Frappier, 531, boul. des Prairies, Laval,
Quebec H7V 1B7, Canada
REFERENCE 3 (bases 1 to 10008)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10008)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
Environ. Microbiol."; date: "2012"; volume: "78"; issue: "17";
pages: "6001-6008"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(23-JAN-2012) Microbiology and Biothechnology, INRS-Institut
Armand-Frappier, 531, boul. des Prairies, Laval, Quebec H7V 1B7,
Canada"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10008
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(281..1807)
/codon_start=1
/gene="tnsD"
/product="Tn7 transposase subunit"
/label="tnsD"
/protein_id="AFM78091.1"
/translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVAT
LGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQGAV
HLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVFFDR
AVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQWTLFY
QRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFRKHRKA
FSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVKHKDWQQ
LVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDWNQRDRIA
VRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKRYSESVEDY
QIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE"
gene complement(281..1807)
/gene="tnsD"
/label="tnsD"
CDS complement(1813..3477)
/gene="tnsC"
/label="Transposon Tn7 transposition protein TnsC"
/note="Transposon Tn7 transposition protein TnsC from
Escherichia coli. Accession#: P05846"
CDS complement(3477..5582)
/gene="tnsB"
/label="Transposon Tn7 transposition protein TnsB"
/note="Transposon Tn7 transposition protein TnsB from
Escherichia coli. Accession#: P13989"
mobile_element 6300..6524
/label="Tn7R"
/note="mini-Tn7 element (right end of the Tn7 transposon)"
promoter complement(6734..6752)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind complement(6788..6821)
/label="FRT (minimal)"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
CDS 6989..7780
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
rep_origin 8649..8871
/label="pSC101 ori"
/note="low-copy replication origin that requires the Rep101
protein"
CDS 8919..9866
/label="Rep101(Ts)"
/note="temperature-sensitive version of the RepA protein
needed for replication with the pSC101 origin (Armstrong et
al., 1984)"
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