LOCUS       V003024                10008 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003024
VERSION     V003024
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10008)
  AUTHORS   Crepin S, Harel J, Dozois CM.
  TITLE     Chromosomal complementation using Tn7 transposon vectors in
            Enterobacteriaceae
  JOURNAL   Appl. Environ. Microbiol. 78 (17), 6001-6008 (2012)
   PUBMED   22706059
REFERENCE   2  (bases 1 to 10008)
  AUTHORS   Crepin S, Harel J, Dozois CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2012) Microbiology and Biothechnology,
            INRS-Institut Armand-Frappier, 531, boul. des Prairies, Laval,
            Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 10008)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10008)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "17";
            pages: "6001-6008"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-JAN-2012) Microbiology and Biothechnology, INRS-Institut
            Armand-Frappier, 531, boul. des Prairies, Laval, Quebec H7V 1B7,
            Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(281..1807)
                     /codon_start=1
                     /gene="tnsD"
                     /product="Tn7 transposase subunit"
                     /label="tnsD"
                     /protein_id="AFM78091.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVAT
                     LGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQGAV
                     HLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVFFDR
                     AVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQWTLFY
                     QRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFRKHRKA
                     FSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVKHKDWQQ
                     LVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDWNQRDRIA
                     VRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKRYSESVEDY
                     QIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE"
     gene            complement(281..1807)
                     /gene="tnsD"
                     /label="tnsD"
     CDS             complement(1813..3477)
                     /gene="tnsC"
                     /label="Transposon Tn7 transposition protein TnsC"
                     /note="Transposon Tn7 transposition protein TnsC from
                     Escherichia coli. Accession#: P05846"
     CDS             complement(3477..5582)
                     /gene="tnsB"
                     /label="Transposon Tn7 transposition protein TnsB"
                     /note="Transposon Tn7 transposition protein TnsB from
                     Escherichia coli. Accession#: P13989"
     mobile_element  6300..6524
                     /label="Tn7R"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     promoter        complement(6734..6752)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6788..6821)
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     CDS             6989..7780
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      8649..8871
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             8919..9866
                     /label="Rep101(Ts)"
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
ORIGIN
        1 cctgcaggtc gactctagag gatcccccga ttattttggc tcgttgaaga tccgatgctt
       61 caaactcaat cacacgatcc gccttattta tttgctttgt tgtactgtta accaccttag
      121 ctcgacttaa taaaggcaaa tgagtaaatc gagtccattt ccgttcttgc attggattaa
      181 accaaacaaa aatacgccac tccttatgac ccgagttacg gaataaatga ccaatatgta
      241 caacctgcac attgtcatta aatgtagcta gcctaaccac tcactcttcc ccataaacca
      301 tttccaagaa tctttgtgct tcctcagtta tccgctcttt agataacgtt gcacttctta
      361 ataatcgcca gcgcctaagc tcaacatctt cctgtttaag cttaataaaa gcttggctaa
      421 tccgtctaat ttgataatct tccacactct ctgagtaacg ctttaagcaa agcgctacca
      481 aaggcagttt ctgtagattt tttgcaagag aggttccgtt aggagtttgc tttaacagcc
      541 agctcgatgt cgctcttggg tgatcaaggc tactatctag acgctttatg attcttaata
      601 gttgtcgtac agcaattcga tctctttggt tccaatcaac tctaggggcg ggtgccagac
      661 gctcttgttg atgctgttga ttccagtgaa ctagccaatc cctgtcatgt cggtaaagcc
      721 aagcgtatag caccccaccc tctaaagact gtcttgccgc cttaattcct tggtatttat
      781 gcactagttg ctgccagtct ttatgcttaa cagataaatc ttcagagtta ggttgcacag
      841 actggctaac aggtcttgtc gttatagagt gctcagtaag agcacttgcc tgctgtagcg
      901 cttctataac cgttagtttt ggcaataagg cttgccacac aatactatgc tgtaaataac
      961 taaaggcttt tctatgctta cggaatatac ttttcagcca acacgtgtcc ttgttctctg
     1021 ccaactttaa atccagtttc tctagtgcct catcactaaa agtctgcctc actctctccg
     1081 ccaccaagtc atgacgaatg tgcttgcttt tggttagccc tagatcctgc gctaagcgct
     1141 gataaaacag cgtccactgc tcaaggcttg gggaaagctc ttgcgctcgt ggagcatcta
     1201 acagaggggc tatataagca gctagtgctg ttaattgaga tagggagtct ttggggtagt
     1261 ctgaaagcag ctcagtatga cccaaagccc aaaattgatg tcggtgatca tctacagctc
     1321 tatcaaagaa gactaaagca ccgtgttttg gacaatatgg caaagcgggc aaataccaat
     1381 ctcgttgcca aaaggcttcc ccatacctat ttagctgaag agcaacgcaa tcagggcagt
     1441 atctaaagcg gttatcgctc ttaactctag aagcagcgac tcctagcatt aaatgcaccg
     1501 caccttgcgc ttggtactcc attaaccgaa tagcttcgtc tcggcgctcc ttgcctacaa
     1561 acggagcata taaagggaat aaggtatgct cataaataag ctgctgaaca gcgtaacgtc
     1621 ctgtttgatg tagatgtctt gctatcacac ctaaatgcga gggcagacct aaggtagcga
     1681 ccaccttgcg gttgccatac acctcatcca acagctgctt aggactaaca atcccttgat
     1741 aaacgcctgc ccgtgcaata gtgctataaa tcagctcatt cgagtacgga acaggaaagt
     1801 ttctcatggc taacctgctt gtttaaataa gctcgccata tccactatta cccctttccc
     1861 ttttaaccgt tcatgcatgg ttttttcagg ttggcgttgt gaatagatat aacgtaaatc
     1921 cgtatcaggc aagctatccc agtcgctggg cttgactacc tttatagccg aaaccttttt
     1981 actcttggag ggcttttcta gttctgacac taccgtttcg ccttccatca accactgcaa
     2041 aacaagaggc agtaactttt gtcttgtcat cgttggattc tggctaaacg cttttttaat
     2101 agtgggaatt aacaggcttg aatcgtaatc ctctttcagc atcagatata aatgacgctg
     2161 atcttcggta tctaactctt gaagggcttt ttcttctggt gtttgttctt gtatcgctgc
     2221 gatatctagc tgaagttgga ttaaccgttt atcaatctcg ggaacgacta gatcagaata
     2281 acgagcaatg cgttctggga tacccgagcg taatgcctct agcatggggt gcacaggctt
     2341 taactcatct tgatacactt gccgcaataa accagcggta atacgctcat tgcctaaagc
     2401 tagcgcacgg agctgagcga gtacaaaaag ttttactaca atgtccatca ctccttggct
     2461 tagctcatac cacacatcac ggacctcatc cgataacagc gcatctttgc gttgtaaaag
     2521 ctgtaattgc caaagattat ccgtaaaagc gatccactct tgattgggct ttccacgttg
     2581 cgtttgttgt ataggatccc agaatatagc tccaaaccct gcccctctac gtgcagaccg
     2641 caaatcagcc tcaaaaatct ctcgtgcttt aggggtacca atcaacatca ctggtacgcc
     2701 aataatattc accatcgtca caaaaaagtt cagcatctct tgagatccac ccgaacgaga
     2761 gcggcttaaa tgctgaattt catcaataac caacaaccct aaagcatgtg cattggctat
     2821 ttgcgacatc aaagccaaca tggtttctat accatgacgt tttaagccat aacgacgctc
     2881 atagttcgag cccaaggctc gatccaacgc tctgaaaaaa ttcaagcaga tttcttttag
     2941 cgaaccatta tgcgagcagt ctattttcaa atacaccacc tgctctacat tgagttcacg
     3001 atggtaaatc acctgaggat acgtggctag aatacgatga agagaggtcg tcttcccact
     3061 accagaacaa ccaattaaca ataagctttg tgccgtagat cgtgcctcct caaagcgaaa
     3121 tgtctccaac tctcccgttt gaacacgctc ataaccattt tgtaaatgct tttgtaaatc
     3181 tcctgtttta ggatttctgc ctacgtagcc acctcgaatc atgaccgaaa tacgctcact
     3241 tagtagcaaa tgcgtaccta atggctgaaa atagtcatct ggaatacgac aaatggtatg
     3301 agctctgata acacgggact tttgcaagtc agaggaagta agctgtaaag aggatttcag
     3361 tgatgcagca ctattcactg actcttgtaa tggtggtaag gcctcgataa aaggattatc
     3421 acgataagcc tctacccccg tatcacgata aactgcttga atccgggtag cactcatgac
     3481 tcatcctttt ctggtggatc ctgaaataat tcaggcacgt atgtgggtag gctgtaatct
     3541 tcttgatcat ccgcttccac tgcgttgaaa ggaataactt tagcctcatc acctgaagag
     3601 cttggcttca aatgctccgc acgtttttta cgctccgagg tcacggcttc ttttttatta
     3661 gttttaatct gcttaatgcg tgttgatttg ggctcagtag tactgggcgt taacttattc
     3721 gctttctgaa tggtttgctg aataaacgcc tcaagctccc tgcgtttagt taactcatcc
     3781 tgcttcgcat tggctttatt gtgtttttct tgtgcttgta tatcccaaac ctcccaaaat
     3841 gagagacctt taaactgccg actacgttcc gtcagattac agcgccaaaa tacacggctg
     3901 ccaacttgcg gaaacaaata aatcgtatca accagcactg ggtcataagc cgcttctaaa
     3961 tgttgaggtc tagctatatc agtgctccgc tgcaaccaac cctcacgcag aatctctgac
     4021 cccgagtaat acaaacccca caaattaacg ccaaatgaag aaatagagac ctttcggcga
     4081 ggcagtaacg ctactcgcaa ctgctcttgc tccacagccc ttaaactacc tgtacgatgc
     4141 tgcatacccc attgccatag ctggacagga atagacggta aatctgtagg aaaatcagca
     4201 tctcgatcgt atttatccat caccagatgg ttatttctga ataagatcgt acgcaaaata
     4261 atttgtgtga actcaaatac cgacagagat gcatctaacc tatagtctgt ttcaccatgg
     4321 cttttgatcc gactgccctc tacaatgcca ggtgcaaagg acttaaactc ggcttgtagt
     4381 gttctaaaag tgctttccac tatgccttta gcatcgccac gtctaggtgg agcactttcc
     4441 actcgcacat taaaactaga aactaaggct tcgacctgat gactcattaa ttcgccacgg
     4501 tccgctagca acacatctgg caaacctaca cacggccagt ctgagctact aatctcaata
     4561 tcatgctggg cacaaatggc cgttttgtca gagcaagcat ttacaaaagc ctgcatcgcc
     4621 accacataag acggattttc aaagccgata taaaagcccg tgatcatccg actaaacaca
     4681 tcaatcacaa tgtaaagcgt tggtcttcct atgatttttt ggcgatcatg atgatccact
     4741 aaataaatat cagcaatcgt ggcatcaatc tcataacgac tcccagggcc taacgcctga
     4801 gaagtggctg tactacttaa gggtcgtacg tcttttttat atacccctgc tttaactcta
     4861 gactttaagc gctgagcttt agggtattct cgatcataaa aataacgaaa ctgacgtagt
     4921 gttgggtaat cctcttgggg aatgcgagga aaatactgag caaacaagtc cacaaatcgt
     4981 ctataggcaa cggtggtctt tgtacctttt tgatttaaca ggtgcttttc tatggtcaac
     5041 ctaaaaaggc gttcaatctc gggcgttacc ttggttcctt cacccttacc atattctctg
     5101 gctcggccaa tctttgctgt tcctgtcgct gaacgtcttt cccctggtgc accgctgttt
     5161 ttgtagtcag gaattaatgc attaggcgtt tgaccacgct gccagtaacg gcgtaacaac
     5221 ttataaactg tagccttagt aaccttatgt tcttggacca catgctcaac gagttcgctt
     5281 ctgactttag ggtcgaaacg atccttacta ttaataatag gaagaatttt tcgataatcc
     5341 tcctcgcgct tctggaagct gacagaatct acagacggct cttctaaatc aagatgtaca
     5401 taagggtcat cagttctaac taagcggcct tcatctaaat actgcattag caacaactca
     5461 gccctagctt gtggaactcc tttatcagcg cttatttgca tccagacaac ttggccatcc
     5521 tctatagcca aaatgcgata cggatcatta tcaaatagca caacctcatt aatttgccac
     5581 atagcgcaac tcctccatat tcactacttg gctaatacag agatctgcac acttatttgc
     5641 cctgaaagac ttataaatat tgaacttaat aaaaccattt gcggttaagg ctcgtatctc
     5701 actcaatgtt ttgcctaact ccaaatcata agcaatatca acctgcttac agacattgat
     5761 aatgttttca tctccttttt cttgcaggat atgggccaat ggggatagtt gtgctaaaag
     5821 ctccgcagaa acttcttctg ttttcactga ataaagccat tcaatatttt cttttactac
     5881 gggatttatt tctttatcag taaaaatgaa ccaaggaatt tgcttttgct gccaatagcg
     5941 acgctctagt tctagttttt ctaaggtacg ctcgtcttgt aaggctgctg caggtttgac
     6001 ttgaatagca aactgctcaa aaggaccatc tttgcagtcc actaaaaaat cagtagacat
     6061 aacctgatct acaccacgaa taacaggatg cttaatacca ctatctattg caatctgcct
     6121 ggtatcacta ggtaataagg ggaactgctc gcgtatatct agcacgctgc tctcccactc
     6181 aagactgaga aaaacagcaa gctctaagtc agatagcaaa tgatggactc gtcccgtctt
     6241 atgagaataa atacggtggg aacgacctga agaaggaact tcttgtactg ttagccatgg
     6301 aatatagtct ttaccatgcc cttggccacg cccctcttta atacgacggg caatttgcac
     6361 ttcagaaaat gaagagtttg ctttagccat aacaaaagtc cagtatgctt tttcacagca
     6421 taactggact gatttcagtt tacaactatt ctgtctagtt taagacttta ttgtcatagt
     6481 ttagatctat tttgttccct ttaagacttt attgtccgcc cacacccgct tacgcagggc
     6541 atccatttat tactcaaccg taaccgattt tgccaggtta cgcggctggt caacgtcggt
     6601 gcctttgatc agcgcgacat ggtaagccag cagctgcagc ggaacggtgt agaagatcgg
     6661 tgcaatcacc tcttccacat gcggcatctc gactctagag gatccccggg taccgagctc
     6721 gaattccggt ctccctatag tgagtcgtat taatttcgat aagccagatc aagcttgtcg
     6781 acaattcgaa gttcctatac tttctagaga ataggaactt cggatcaatt catcgatgca
     6841 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6901 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atctagtgga
     6961 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     7021 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     7081 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     7141 cgacctgtcc ggtgccctga atgaactcca agacgaggca gcgcggctat cgtggctggc
     7201 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     7261 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     7321 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     7381 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     7441 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     7501 cgccaggctc aaggcgcgga tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     7561 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     7621 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     7681 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     7741 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     7801 gataccgtca aaacttcatt tttaattttt gcggcccggc tggctggttt attgctgata
     7861 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7921 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7981 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8041 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8101 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8161 gagcgtcaga ccccgttgat gataccgctg ccttactggg tgcattagcc agtctgaatg
     8221 acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag gaactgctga
     8281 acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc tgagaagccc
     8341 gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa aaggcgcctg
     8401 tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg aatgtcacga
     8461 aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca gcgatttgcc
     8521 cgattgcggc cgcaaccgag cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc
     8581 tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc ggaactgact
     8641 aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt tattctttct
     8701 ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg
     8761 aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag cagaatttac
     8821 agatacccac aactcaaagg aaaaggacta gtaattatca ttgactagcc catctcaatt
     8881 ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta gttgttttca
     8941 aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc aagctaattt
     9001 tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg
     9061 tatcgttcac ttataaccaa tacgttcaga tgatgaacat cagtagggaa aatgcttatg
     9121 gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc aggaatcctt
     9181 tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca agcgaaaaat
     9241 tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa aaattcataa
     9301 aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg atttatgagt
     9361 ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag attagccttg
     9421 atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa aggcttaacc
     9481 aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg aaattggtgg
     9541 ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta gatagacaaa
     9601 tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac aaaataccaa
     9661 caaccattac atcagattcc tacctacgta acggactaag aaaaacacta cacgatgctt
     9721 taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac atgcaaagta
     9781 agcatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc acactagaga
     9841 acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc tatcagtgaa
     9901 gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc aaagggaaaa
     9961 ctgtccatac ccatgggcta gctgatcagc cagtgccaag cttgcatg
//
