pSR26-2 vector (V003057)

LOCUS       V003057                 3624 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003057
VERSION     V003057
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Landry BP, Palanki R, Dyulgyarov N, Hartsough LA, Tabor JJ.
  TITLE     Phosphatase activity tunes two-component system sensor detection
            threshold
  JOURNAL   Nat Commun 9 (1), 1433 (2018)
   PUBMED   29650958
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Landry BP.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2017) Bioengineering, Rice University, 6100 Main
            St, Houston, TX 77005, USA
REFERENCE   3  (bases 1 to 3624)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3624)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun"; date: "2018"; volume: "9"; issue: "1"; pages: "1433"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-OCT-2017) Bioengineering, Rice University, 6100 Main St,
            Houston, TX 77005, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..6)
                     /label="AvrII Scar"
                     /note="AvrII Scar"
     rep_origin      complement(151..739)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(763..815)
                     /label="Tn3 Artifact"
                     /note="Tn3 Artifact"
     misc_feature    complement(815..820)
                     /label="SpeI Scar"
                     /note="SpeI Scar"
     terminator      complement(827..921)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     misc_feature    complement(927..932)
                     /label="SacI Scar"
                     /note="SacI Scar"
     CDS             complement(945..1601)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(1602..1704)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     misc_feature    1751..1801
                     /label="BW25113 LacZ Upstream"
                     /note="BW25113 LacZ Upstream"
     misc_feature    1803..1808
                     /label="XhoI Scar"
                     /note="XhoI Scar"
     promoter        1809..1882
                     /label="PLtetO-1 promoter"
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     regulatory      1883..1917
                     /label="sRBS (500)"
                     /note="sRBS (500)"
                     /regulatory_class="ribosome_binding_site"
     CDS             1918..2634
                     /gene="ompR"
                     /label="DNA-binding dual transcriptional regulator OmpR"
                     /note="DNA-binding dual transcriptional regulator OmpR from
                     Salmonella typhimurium (strain SL1344). Accession#:
                     A0A0H3NGY1"
     terminator      2658..2729
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    2730..2737
                     /label="BB Scar"
                     /note="BB Scar"
     terminator      2745..2772
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    2779..2823
                     /label="pUC MCS"
                     /note="pUC MCS"
     regulatory      2824..2858
                     /label="J23106"
                     /note="J23106"
                     /regulatory_class="promoter"
     regulatory      2859..2892
                     /label="sRBS (50,000)"
                     /note="sRBS (50,000)"
                     /regulatory_class="ribosome_binding_site"
     CDS             2893..3516
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     terminator      3525..3611
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"