LOCUS       V003057                 3624 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003057
VERSION     V003057
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Landry BP, Palanki R, Dyulgyarov N, Hartsough LA, Tabor JJ.
  TITLE     Phosphatase activity tunes two-component system sensor detection
            threshold
  JOURNAL   Nat Commun 9 (1), 1433 (2018)
   PUBMED   29650958
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Landry BP.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2017) Bioengineering, Rice University, 6100 Main
            St, Houston, TX 77005, USA
REFERENCE   3  (bases 1 to 3624)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3624)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun"; date: "2018"; volume: "9"; issue: "1"; pages: "1433"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-OCT-2017) Bioengineering, Rice University, 6100 Main St,
            Houston, TX 77005, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..6)
                     /label="AvrII Scar"
                     /note="AvrII Scar"
     rep_origin      complement(151..739)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(763..815)
                     /label="Tn3 Artifact"
                     /note="Tn3 Artifact"
     misc_feature    complement(815..820)
                     /label="SpeI Scar"
                     /note="SpeI Scar"
     terminator      complement(827..921)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     misc_feature    complement(927..932)
                     /label="SacI Scar"
                     /note="SacI Scar"
     CDS             complement(945..1601)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(1602..1704)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     misc_feature    1751..1801
                     /label="BW25113 LacZ Upstream"
                     /note="BW25113 LacZ Upstream"
     misc_feature    1803..1808
                     /label="XhoI Scar"
                     /note="XhoI Scar"
     promoter        1809..1882
                     /label="PLtetO-1 promoter"
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     regulatory      1883..1917
                     /label="sRBS (500)"
                     /note="sRBS (500)"
                     /regulatory_class="ribosome_binding_site"
     CDS             1918..2634
                     /gene="ompR"
                     /label="DNA-binding dual transcriptional regulator OmpR"
                     /note="DNA-binding dual transcriptional regulator OmpR from
                     Salmonella typhimurium (strain SL1344). Accession#:
                     A0A0H3NGY1"
     terminator      2658..2729
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    2730..2737
                     /label="BB Scar"
                     /note="BB Scar"
     terminator      2745..2772
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    2779..2823
                     /label="pUC MCS"
                     /note="pUC MCS"
     regulatory      2824..2858
                     /label="J23106"
                     /note="J23106"
                     /regulatory_class="promoter"
     regulatory      2859..2892
                     /label="sRBS (50,000)"
                     /note="sRBS (50,000)"
                     /regulatory_class="ribosome_binding_site"
     CDS             2893..3516
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     terminator      3525..3611
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 cctaggcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
       61 cacagaatca ggggataacg catgcaagaa catgtgagca aaaggccagc aaaaggccag
      121 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      181 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      241 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      301 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
      361 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
      421 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
      481 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      541 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
      601 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
      661 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
      721 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
      781 gaacgaaaac tcacgttaag ggattttggt catgactagt gcttggattc tcaccaataa
      841 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
      901 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
      961 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     1021 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     1081 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     1141 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
     1201 aaataggcca ggttttcacc ataacacgcc acatcttgcg aatatatgtg tagaaactgc
     1261 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     1321 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     1381 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     1441 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     1501 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     1561 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     1621 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     1681 ttggaacctc ttacgtgccg atcaacgtct cattttcgcc agatatcgac gtctaagaaa
     1741 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     1801 acctcgagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
     1861 acatcagcag gacgcactga ccgcataaag gacttagcaa agcccaattt taaacaaatg
     1921 caagagaact acaagattct ggtggtcgat gacgacatgc gcctgcgtgc gctgctggaa
     1981 cgttatctca ccgaacaagg cttccaggtt cgaagcgtcg ctaatgcaga acagatggat
     2041 cgcctgctga ctcgtgaatc tttccatctt atggtactgg atttaatgtt acctggtgaa
     2101 gatggcttgt cgatttgccg acgtcttcgt agtcagagca acccgatgcc gatcattatg
     2161 gtgacggcga aaggggaaga agtggaccgt atcgtaggcc tggagattgg cgctgacgac
     2221 tacattccaa aaccgtttaa cccgcgtgaa ctgctggccc gtatccgtgc ggtgctgcgt
     2281 cgtcaggcga acgaactgcc aggcgcaccg tcacaggaag aggcggtaat tgctttcggt
     2341 aagttcaaac ttaacctcgg tacgcgcgaa atgttccgcg aagacgagcc gatgccgctc
     2401 accagcggtg agtttgcggt actgaaggca ctggtcagcc atccgcgtga gccgctctcc
     2461 cgcgataagc tgatgaacct tgcccgtggt cgtgaatatt ccgcaatgga acgctccatc
     2521 gacgtgcaga tttcgcgtct gcgccgcatg gtggaagaag atccagcgca tccgcgttac
     2581 attcagaccg tctggggtct gggctacgtc tttgtaccgg acggctctaa agcatgataa
     2641 taatctagac caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2701 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2761 ggcctttctg cgtttatagg tacccgtgga tcctctagag tcgacctgca ggcatgcaag
     2821 ctttttacgg ctagctcagt cctaggtata gtgctagccc agccagagaa acactcttta
     2881 acagggggta gtatgatgtc tcgtttagat aaaagtaaag tgattaacag cgcattagag
     2941 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
     3001 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
     3061 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
     3121 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
     3181 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
     3241 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
     3301 gcagtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
     3361 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
     3421 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
     3481 ggattagaaa aacaacttaa atgtgaaagt gggtcttaag gcatcaaata aaacgaaagg
     3541 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     3601 gtaggacaaa tccgccgccc taga
//
