Basic Vector Information
Stat3-C Flag pRc/CMV vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Stat3-C Flag pRc/CMV vector Sequence
LOCUS Stat3-C_Flag_pRc 7969 bp DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS Stat3-C Flag pRc/CMV.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7969)
AUTHORS Bromberg JF, Wrzeszczynska MH, Devgan G, Zhao Y, Pestell RG,
Albanese C, Darnell JE Jr
TITLE Stat3 as an oncogene.
JOURNAL Cell 1999 Aug 6;98(3):295-303.
PUBMED 10458605
REFERENCE 2 (bases 1 to 7969)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 7969)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell";
date: "1999-08-6"; volume: "98(3)"; pages: "295-303"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7969
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter complement(534..638)
/label=AmpR promoter
enhancer 904..1283
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1284..1487
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 1532..1550
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 1641..1657
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
CDS 1749..4058
/gene="Stat3"
/label=Stat3
/note="Signal transducer and activator of transcription 3
from Mus musculus. Accession#: P42227"
CDS 4065..4088
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
promoter complement(4097..4115)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
polyA_signal 4141..4365
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4411..4924
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4952..5281
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 5348..6139
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 6316..6450
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6481..6497)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(6505..6521)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6529..6559)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(6574..6595)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6883..7471)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(join(7645..7969,1..533))
/label=AmpR
/note="beta-lactamase"
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