LOCUS       Stat3-C_Flag_pRc        7969 bp DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    Stat3-C Flag pRc/CMV.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Bromberg JF, Wrzeszczynska MH, Devgan G, Zhao Y, Pestell RG, 
            Albanese C, Darnell JE Jr
  TITLE     Stat3 as an oncogene.
  JOURNAL   Cell 1999 Aug 6;98(3):295-303.
  PUBMED    10458605
REFERENCE   2  (bases 1 to 7969)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7969)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "1999-08-6"; volume: "98(3)"; pages: "295-303"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(534..638)
                     /label=AmpR promoter
     enhancer        904..1283
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1284..1487
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        1532..1550
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1641..1657
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1749..4058
                     /gene="Stat3"
                     /label=Stat3
                     /note="Signal transducer and activator of transcription 3
                     from Mus musculus. Accession#: P42227"
     CDS             4065..4088
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     promoter        complement(4097..4115)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    4141..4365
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4411..4924
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4952..5281
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5348..6139
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    6316..6450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6481..6497)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6505..6521)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6529..6559)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6574..6595)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6883..7471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7645..7969,1..533))
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
       61 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
      121 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
      181 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
      241 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
      301 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
      361 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
      421 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
      481 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
      541 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      601 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      661 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
      721 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
      781 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
      841 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
      901 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
      961 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1021 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1081 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     1141 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     1201 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     1261 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     1321 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     1381 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     1441 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
     1501 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
     1561 cttggtaccg agctcggatc cactagtaac ggccgccagt gtgctggaat tctgcagata
     1621 tccatcacac tggcggccgc tctagaacta gtggatcccc cgggctgcag gaattccgcc
     1681 gcgaccagcg caggccggcc agtcgggcct cagccccgga gacagtcgag acccctgact
     1741 gcagcaggat ggctcagtgg aaccagctgc agcagctgga cacacgctac ctgaagcagc
     1801 tgcaccagct gtacagcgac agcttcccca tggagctgcg gcagttcctg gcaccttgga
     1861 ttgagagtca agactgggca tatgcagcca gcaaagagtc acatgccacg ttggtgtttc
     1921 ataatctctt gggtgaaatt gaccagcaat atagccgatt cctgcaagag tccaatgtcc
     1981 tctatcagca caaccttcga agaatcaagc agtttctgca gagcaggtat cttgagaagc
     2041 caatggaaat tgcccggatc gtggcccgat gcctgtggga agagtctcgc ctcctccaga
     2101 cggcagccac ggcagcccag caagggggcc aggccaacca cccaacagcc gccgtagtga
     2161 cagagaagca gcagatgttg gagcagcatc ttcaggatgt ccggaagcga gtgcaggatc
     2221 tagaacagaa aatgaaggtg gtggagaacc tccaggacga ctttgatttc aactacaaaa
     2281 ccctcaagag ccaaggagac atgcaggatc tgaatggaaa caaccagtct gtgaccagac
     2341 agaagatgca gcagctggaa cagatgctca cagccctgga ccagatgcgg agaagcattg
     2401 tgagtgagct ggcggggctc ttgtcagcaa tggagtacgt gcagaagaca ctgactgatg
     2461 aagagctggc tgactggaag aggcggcagc agatcgcgtg catcggaggc cctcccaaca
     2521 tctgcctgga ccgtctggaa aactggataa cttcattagc agaatctcaa cttcagaccc
     2581 gccaacaaat taagaaactg gaggagctgc agcagaaagt gtcctacaag ggcgacccta
     2641 tcgtgcagca ccggcccatg ctggaggaga ggatcgtgga gctgttcaga aacttaatga
     2701 agagtgcctt cgtggtggag cggcagccct gcatgcccat gcacccggac cggcccttag
     2761 tcatcaagac tggtgtccag tttaccacga aagtcaggtt gctggtcaaa tttcctgagt
     2821 tgaattatca gcttaaaatt aaagtgtgca ttgataaaga ctctggggat gttgctgccc
     2881 tcagagggtc tcggaaattt aacattctgg gcacgaacac aaaagtgatg aacatggagg
     2941 agtctaacaa cggcagcctg tctgcagagt tcaagcacct gacccttagg gagcagagat
     3001 gtgggaatgg aggccgtgcc aattgtgatg cctccttgat cgtgactgag gagctgcacc
     3061 tgatcacctt cgagactgag gtgtaccacc aaggcctcaa gattgaccta gagacccact
     3121 ccttgccagt tgtggtgatc tccaacatct gtcagatgcc aaatgcttgg gcatcaatcc
     3181 tgtggtataa catgctgacc aataacccca agaacgtgaa cttcttcact aagccgccaa
     3241 ttggaacctg ggaccaagtg gccgaggtgc tcagctggca gttctcgtcc accaccaagc
     3301 gagggctgag catcgagcag ctgacaacgc tggctgagaa gctcctaggg cctggtgtga
     3361 actactcagg gtgtcagatc acatgggcta aattctgcaa agaaaacatg gctggcaagg
     3421 gcttctcctt ctgggtctgg ctagacaata tcatcgacct tgtgaaaaag tatatcttgg
     3481 ccctttggaa tgaagggtac atcatgggtt tcatcagcaa ggagcgggag cgggccatcc
     3541 taagcacaaa gcccccgggc accttcctac tgcgcttcag cgagagcagc aaagaaggag
     3601 gggtcacttt cacttgggtg gaaaaggaca tcagtggcaa gacccagatc cagtctgtag
     3661 agccatacac caagcagcag ctgaacaaca tgtcatttgc tgaaatcatc atgggctata
     3721 agatcatgga ttgtacctgc atcctggtgt ctccacttgt ctacctctac cccgacattc
     3781 ccaaggagga ggcatttgga aagtactgta ggcccgagag ccaggagcac cccgaagccg
     3841 acccaggtag tgctgccccg tacctgaaga ccaagttcat ctgtgtgaca ccaacgacct
     3901 gcagcaatac cattgacctg ccgatgtccc cccgcacttt agattcattg atgcagtttg
     3961 gaaataacgg tgaaggtgct gagccctcag caggagggca gtttgagtcg ctcacgtttg
     4021 acatggatct gacctcggag tgtgctacct cccccatgtc tagagactac aaggacgacg
     4081 atgacaaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
     4141 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     4201 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     4261 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     4321 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     4381 gaaccagctg gggctcgagg ggggatcccc acgcgccctg tagcggcgca ttaagcgcgg
     4441 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4501 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4561 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4621 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4681 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4741 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4801 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     4861 caatttaaat atttgcttat acaatcttcc tgtttttggg gcttttctga ttatcaaccg
     4921 gggtgggtac cgagctcgaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     4981 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5041 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5101 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5161 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5221 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     5281 aaagctcccg ggagcttgga tatccatttt cggatctgat caagagacag gatgaggatc
     5341 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     5401 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5461 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5521 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5581 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5641 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     5701 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     5761 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     5821 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     5881 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     5941 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     6001 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     6061 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     6121 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     6181 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     6241 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     6301 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6361 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6421 ctcatcaatg tatcttatca tgtctggatc cgtcgacctc gagagcttgg cgtaatcatg
     6481 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6541 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6601 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     6661 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6781 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7321 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7441 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7501 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7561 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7621 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7681 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7741 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7801 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7861 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7921 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtg
//
