pSOL9DEF2 vector (V003127)

LOCUS       V003127                 9112 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003127
VERSION     V003127
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Weinhold A, Wielsch N, Svato A, Baldwin IT.
  TITLE     Label-free nanoUPLC-MS E based quantification of antimicrobial
            peptides from the leaf apoplast of Nicotiana attenuata
  JOURNAL   BMC Plant Biol. 15 (1), 18 (2015)
   PUBMED   25604123
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   Gase K, Weinhold A, Baldwin IT.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2013) Molecular Ecology, Max-Planck-Institute for
            Chemical Ecology, Hans-Knoell-Strasse 8, Jena, Thueringen 07745,
            Germany
REFERENCE   3  (bases 1 to 9112)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9112)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant
            Biol."; date: "2015"; volume: "15"; issue: "1"; pages: "18"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-DEC-2013) Molecular Ecology, Max-Planck-Institute for Chemical
            Ecology, Hans-Knoell-Strasse 8, Jena, Thueringen 07745, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    244..268
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(342..594)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(619..1641)
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(1662..1845)
                     /label="NOS promoter"
                     /note="nopaline synthase promoter"
     promoter        2155..2500
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2521..2835
                     /gene="D1"
                     /label="Flower-specific defensin"
                     /note="Flower-specific defensin from Nicotiana alata.
                     Accession#: Q8GTM0"
     polyA_signal    2853..3027
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    3099..3123
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      3480..4068
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      complement(4511..4705)
                     /direction=LEFT
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4774..5844)
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6276..6902)
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             8312..9103
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"