LOCUS       V003127                 9112 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003127
VERSION     V003127
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Weinhold A, Wielsch N, Svato A, Baldwin IT.
  TITLE     Label-free nanoUPLC-MS E based quantification of antimicrobial
            peptides from the leaf apoplast of Nicotiana attenuata
  JOURNAL   BMC Plant Biol. 15 (1), 18 (2015)
   PUBMED   25604123
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   Gase K, Weinhold A, Baldwin IT.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2013) Molecular Ecology, Max-Planck-Institute for
            Chemical Ecology, Hans-Knoell-Strasse 8, Jena, Thueringen 07745,
            Germany
REFERENCE   3  (bases 1 to 9112)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9112)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant
            Biol."; date: "2015"; volume: "15"; issue: "1"; pages: "18"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-DEC-2013) Molecular Ecology, Max-Planck-Institute for Chemical
            Ecology, Hans-Knoell-Strasse 8, Jena, Thueringen 07745, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    244..268
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(342..594)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(619..1641)
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(1662..1845)
                     /label="NOS promoter"
                     /note="nopaline synthase promoter"
     promoter        2155..2500
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2521..2835
                     /gene="D1"
                     /label="Flower-specific defensin"
                     /note="Flower-specific defensin from Nicotiana alata.
                     Accession#: Q8GTM0"
     polyA_signal    2853..3027
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    3099..3123
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      3480..4068
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      complement(4511..4705)
                     /direction=LEFT
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4774..5844)
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6276..6902)
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             8312..9103
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gaattcgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
       61 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
      121 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg
      181 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
      241 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
      301 gcggacgttt ttaatgtact ggcgcgccgg atccaattcc cgatctagta acatagatga
      361 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta
      421 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta
      481 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag
      541 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggaa
      601 attcgagctg gtaccctatt tctttgccct cggacgagtg ctggggcgtc ggtttccact
      661 atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg
      721 tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca
      781 agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg
      841 gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc gctcgaagta
      901 gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc
      961 gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt
     1021 gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca
     1081 gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc
     1141 gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg
     1201 ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct
     1261 aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa cagcgggcag
     1321 ttcggtttca ggcaggtctt gcaacgtgac accctgtgaa cggcgggaga tgcaataggt
     1381 caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg cggccgatgc
     1441 aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag
     1501 gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag
     1561 ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc
     1621 ggtgagttca ggctttttca tgcgaaacga tccagatccg gtgcagatta tttggattga
     1681 gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat
     1741 ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata
     1801 atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc tgagtggctc
     1861 cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg
     1921 ggggtcataa cgtgactccc ttaattctcc gctcatgatc ctctagagct tcatggagtc
     1981 aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca
     2041 gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac
     2101 acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac
     2161 ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca
     2221 ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa
     2281 aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
     2341 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     2401 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
     2461 ttcctctata taaggaagtt catttcattt ggagagaaca cgggggactc ttgactcgag
     2521 atggctcgct ccttgtgctt catggcattt gctatcttgg caatgatgct ctttgttgcc
     2581 tatgaggtgc aagctagaga atgcaaaaca gaaagcaaca catttcctgg aatatgcatt
     2641 accaaaccac cttgcagaaa agcttgtatc agtgagaaat ttactgatgg tcattgtagc
     2701 aaaatcctca gaaggtgtct atgcactaag ccatgtgtgt ttgatgagaa gatgactaaa
     2761 actggagctg aaattttggc tgaggaagca aaaactttgg ctgcagcttt gcttgaagaa
     2821 gagatgatgg ataactaagg tgaccggtcg agtttctcca taataatgtg tgagtagttc
     2881 ccagataagg gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac
     2941 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
     3001 ccaaaatcca gtactaaaat ccagatcccc cgaattaatg agcttggcgc gccggatcag
     3061 attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta
     3121 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     3181 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttccccaga tccgtagagt
     3241 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     3301 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     3361 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3421 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3481 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3541 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3601 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3661 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3721 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3781 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3841 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3901 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3961 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4021 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4081 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgta cactccgcta
     4141 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     4201 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     4261 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg
     4321 tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg
     4381 ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg
     4441 gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg
     4501 gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt
     4561 ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt
     4621 cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg
     4681 gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag
     4741 ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat
     4801 caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc
     4861 gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa
     4921 ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt
     4981 gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa
     5041 gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat
     5101 ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt
     5161 gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt
     5221 cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc
     5281 gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg
     5341 acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt
     5401 tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg
     5461 ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg
     5521 tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt
     5581 gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt
     5641 cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc
     5701 ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc
     5761 ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg
     5821 gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg
     5881 cagggccggc aggcaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg
     5941 gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc
     6001 gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta
     6061 ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt
     6121 gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct
     6181 ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt
     6241 gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg
     6301 gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg
     6361 ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc
     6421 agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc
     6481 cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc
     6541 agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc
     6601 acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg
     6661 atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg
     6721 atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc
     6781 tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga
     6841 tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc
     6901 atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat
     6961 gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt
     7021 cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt
     7081 catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca
     7141 tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt
     7201 tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa
     7261 tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg
     7321 ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac
     7381 ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt
     7441 agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg
     7501 gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg
     7561 gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc
     7621 ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag
     7681 cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg
     7741 gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg
     7801 cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag
     7861 caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt
     7921 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
     7981 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
     8041 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
     8101 ggaaccctgt ggttggcatg cataattgtg gtttcaaaat cggctccgtc gatactatgt
     8161 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
     8221 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
     8281 aatactgtag aaaagaggaa ggaaataata aatgattgaa caagatggat tgcacgcagg
     8341 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     8401 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     8461 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     8521 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     8581 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     8641 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     8701 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     8761 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     8821 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     8881 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     8941 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     9001 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     9061 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctagtacc ta
//
