pSMT3-EO6 vector (V003132)

LOCUS       V003132                 6888 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003132
VERSION     V003132
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2005) Medical Virology, University of Cape Town,
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 6888)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6888)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-JUL-2005) Medical Virology, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      6..381
                     /note="derived from Mycobacterium bovis hsp60 promoter of
                     GenBank Accession Number M17705"
                     /regulatory_class="promoter"
     misc_feature    381..398
                     /label="derived from Mycobacterium bovis hsp60 protein"
                     /note="derived from Mycobacterium bovis hsp60 protein"
     CDS             408..1598
                     /note="Intermediate capsid protein VP6 from Rotavirus A
                     (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]).
                     Accession#: B2BN53"
                     /label="Intermediate capsid protein VP6"
     primer_bind     complement(1613..1629)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      1659..4246
                     /label="Mycobacterial OriM region derived from pAL5000"
                     /note="Mycobacterial OriM region derived from pAL5000"
     CDS             complement(4487..5482)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     rep_origin      5852..6440
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6728..6749
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6764..6794
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6802..6818
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6826..6842
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"