LOCUS       V003132                 6888 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003132
VERSION     V003132
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2005) Medical Virology, University of Cape Town,
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 6888)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6888)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-JUL-2005) Medical Virology, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      6..381
                     /note="derived from Mycobacterium bovis hsp60 promoter of
                     GenBank Accession Number M17705"
                     /regulatory_class="promoter"
     misc_feature    381..398
                     /label="derived from Mycobacterium bovis hsp60 protein"
                     /note="derived from Mycobacterium bovis hsp60 protein"
     CDS             408..1598
                     /note="Intermediate capsid protein VP6 from Rotavirus A
                     (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]).
                     Accession#: B2BN53"
                     /label="Intermediate capsid protein VP6"
     primer_bind     complement(1613..1629)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      1659..4246
                     /label="Mycobacterial OriM region derived from pAL5000"
                     /note="Mycobacterial OriM region derived from pAL5000"
     CDS             complement(4487..5482)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     rep_origin      5852..6440
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6728..6749
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6764..6794
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6802..6818
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6826..6842
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 ctagaggtga ccacaacgac gcgcccgctt tgatcgggga cgtctgcggc cgaccattta
       61 cgggtcttgt tgtcgttggc ggtcatgggc cgaacatact cacccggatc ggagggccga
      121 ggacaaggtc gaacgagggg catgacccgg tgcggggctt cttgcactcg gcataggcga
      181 gtgctaagaa taacgttggc actcgcgacc ggtgagtcgt aggtcgggac ggtgaggcca
      241 ggcccgtcgt cgcagcgagt ggcagcgagg acaacttgag ccgtccgtcg cgggcactgc
      301 gcccggccag cgtaagtagc ggggttgccg tcacccggtg acccccggtt tcatccccga
      361 tccggaggaa tcacttcgca atggccaaga caattgcgga tcccaaaatg gatgtgctgt
      421 actccatctc gagaacactg aaggatgcta gggataaaat tgttgagggt acgttgtact
      481 ctaacgttag tgatcttatt cagcagttca atcagatgct ggtcacaatg aatggaaatg
      541 aatttcaaac ggggggaatt ggtaacttgc cacttcgtaa ttggaacttt gattttggac
      601 tactaggtac tacgctatta aatctagacg caaactacgt tgaatcagcc agaaccacaa
      661 ttgattattt tgttgatttc atagataatg tatgtatgga tgaaatggtg cgagagtcgc
      721 aaaggaatgg aatagcacca caatcagatg ccttgcggaa gttatcagga gttaagttca
      781 gacgaataaa ctttaacaat tcatcagagt atattgagaa ctggaacctt cagaaccgca
      841 ggcaaaggac gggattcacg tttcacaaac cgaacatatt tccatattcg gcatcattca
      901 cattgaatag gtcgcagccg cagcatgaca acttaatggg cacaatgtgg ctaaacgctg
      961 ggtcagaaat acaggttgca ggatttgact attcatgcgc aattaacgct ccagctaaca
     1021 ttcaacaatt tgaacatata gtgcaattac ggagggtatt gactactgca accattacgc
     1081 tacttccgga tgcagagagg tttagtttcc caagagttat taactcagca gacggagcaa
     1141 ctacgtggta tttcaaccca gtaatattgc gtccaaataa tgtagaagtt gaattcttat
     1201 tgaatggtca gatcatcaat acttaccaag ctagattcgg taccatcgtc gcaagaaatt
     1261 ttgataccat acgcctttct tttcagctga tgagaccacc aaacatgaca ccagcagtgg
     1321 ctgcactttt cccgaatgct caaccgttcg agcatcatgc aacagtcgga ctgacgctta
     1381 ggattgactc agcaatttgt gaatcagttc ttgcagacgc aagtgaaact atgttggcta
     1441 atgtgacgtc agtcagacaa gaatacgcaa taccagtagg accagtattt ccaccaggaa
     1501 tgaattggac cgacctcatt actaattatt caccatcaag ggaggacaat ctgcaacgtg
     1561 tgtttacagt agcttccatt agaagcatgt tggtaaagtg agcgaagctt atcgataccg
     1621 tcgacctcga gggggggccc ggtacgtacc cggggatcat cgagccgaga acgttatcga
     1681 agttggtcat gtgtaatccc ctcgtttgaa ctttggatta agcgtagata cacccttgga
     1741 caagccagtt ggattcggag acaagcaaat tcagccttaa aaagggcgag gcctgcggtg
     1801 gtggaacacc gcagggcctc taaccgctcg acgcgctgca ccaaccagcc cgcgaacggc
     1861 tggcagccag cgtaaggcgc ggctcatcgg gcggcgttcg ccacgatgtc ctgcacttcg
     1921 agccaagcct cgaacacctg ctggtgtgca cgactcaccc ggttgttgac accgcgcgcg
     1981 gccgtgcggg ctcggtgggg cggctgtgtc gcccttgcca gcgtgagtag cgcgtacctc
     2041 acctcgccca acaggtcgca cacagccgat tcgtacgcca taaagccagg tgagcccacc
     2101 agctccgtaa gttcgggcgc tgtgtggctc gtacccgcgc attcaggcgg cagggggtct
     2161 aacgggtcta aggcggcgtg tacggccgcc acagcggctc tcagcggccc ggaaacgtcc
     2221 tcgaaacgac gcatgtgttc ctcctggttg gtacaggtgg ttgggggtgc tcggctgtcg
     2281 ctggtgttcc accaccaggg ctcgacggga gagcggggga gtgtgcagtt gtggggtggc
     2341 ccctcagcga aatatctgac ttggagctcg tgtcggacca tacaccggtg attaatcgtg
     2401 gtctactacc aagcgtgagc cacgtcgccg acgaatttga gcagctctgg ctgccgtact
     2461 ggccgctggc aagcgacgat ctgctcgagg ggatctaccg ccaaagccgc gcgtcggccc
     2521 taggccgccg gtacatcgag gcgaacccaa cagcgctggc aaacctgctg gtcgtggacg
     2581 tagaccatcc agacgcagcg ctccgagcgc tcagcgcccg ggggtcccat ccgctgccca
     2641 acgcgatcgt gggcaatcgc gccaacggcc acgcacacgc agtgtgggca ctcaacgccc
     2701 ctgttccacg caccgaatac gcgcggcgta agccgctcgc atacatggcg gcgtgcgccg
     2761 aaggccttcg gcgcgccgtc gacggcgacc gcagttactc aggcctcatg accaaaaacc
     2821 ccggccacat cgcctgggaa acggaatggc tccactcaga tctctacaca ctcagccaca
     2881 tcgaggccga gctcggcgcg aacatgccac cgccgcgctg gcgtcagcag accacgtaca
     2941 aagcggctcc gacgccgcta gggcggaatt gcgcactgtt cgattccgtc aggttgtggg
     3001 cctatcgtcc cgccctcatg cggatctacc tgccgacccg gaacgtggac ggactcggcc
     3061 gcgcgatcta tgccgagtgc cacgcgcgaa acgccgaatt cccgtgcaac gacgtgtgtc
     3121 ccggaccgct accggacagc gaggtccgcg ccatcgccaa cagcatttgg cgttggatca
     3181 caaccaagtc gcgcatttgg gcggacggga tcgtggtcta cgaggccaca ctcagtgcgc
     3241 gccagtcggc catctcgcgg aagggcgcag cagcgcgcac ggcggcgagc acagttgcgc
     3301 ggcgcgcaaa gtccgcgtca gccatggagg cattgctatg agcgacggct acagcgacgg
     3361 ctacagcgac ggctacaacc ggcagccgac tgtccgcaaa aagcggcgcg tgaccgccgc
     3421 cgaaggcgct cgaatcaccg gactatccga acgccacgtc gtccggctcg tggcgcagga
     3481 acgcagcgag tggctcgccg agcaggctgc acgccgcgaa cgcatccgcg cctatcacga
     3541 cgacgagggc cactcttggc cgcaaacggc caaacatttc gggctgcatc tggacaccgt
     3601 taagcgactc ggctatcggg cgaggaaaga gcgtgcggca gaacaggaag cggctcaaaa
     3661 ggcccacaac gaagccgaca atccaccgct gttctaacgc aattggggag cgggtgtcgc
     3721 gggggttccg tggggggttc cgttgcaacg ggtcggacag gtaaaagtcc tggtagacgc
     3781 tagttttctg gtttgggcca tgcctgtctc gttgcgtgtt tcgttgcgcc cgttttgaat
     3841 accagccaga cgagacgggg ttctacgaat cttggtcgat accaagccat ttccgctgaa
     3901 tatcggggag ctcaccgcca gaatcggtgg ttgtggtgat gtacgtggcg aactccgttg
     3961 tagtgcctgt ggtggcatcc gtggccactc tcgttgcacg gttcgttgtg ccgttacagg
     4021 ccccgttgac agctcaccga acgtagttaa aacatgctgg tcaaactagg tttaccaacg
     4081 atacgagtca gctcatctag ggccagttct aggcgttgtt cgttgcgcgg ttcgttgcgc
     4141 atgtttcgtg tggttgctag atggctccgc aaccacacgc ttcgaggttg agtgcttcca
     4201 gcacgggcgc gatccagaag aacttcgtcg tgcgactgtc ctcgttgatc cttgccgagc
     4261 tgggatggaa gctcggccga ccaccctgga ggagatgatc gaggatgcca gggcctttca
     4321 cgcccgccgc tgctgagcgt ccgccgccgg gcccgcaccg ccgtcggccg gcccgctccg
     4381 ggctcgcagc agcgggcttc ggcgcgggcc cggggctccc gagcgcgggc ggggctccgg
     4441 gcggccgccg ggggccgggg gcggcgccgg gcggcccggg gcgtcaggcg ccgggggcgg
     4501 tgtccggcgg cccccagagg aactgcgcca gttcctccgg atcggtgaag ccggagagat
     4561 ccagcggggt ctcctcgaac acctcgaagt cgtgcaggaa ggtgaaggcg agcagttcgc
     4621 gggcgaagtc ctcggtccgc ttccactgcg ccccgtcgag cagcgcggcc aggatctcgc
     4681 ggtcgccccg gaaggcgttg agatgcagtt gcaccaggct gtagcgggag tctcccgcat
     4741 agacgtcggt gaagtcgacg atcccggtga cctcggtcgc ggccaggtcc acgaagatgt
     4801 tggtcccgtg caggtcgccg tggacgaacc ggggttcgcg gccggccagc agcgtgtcca
     4861 cgtccggcag ccagtcctcc aggcggtcca gcagccgggg cgagaggtag ccccacccgc
     4921 ggtggtcctc gacggtcgcc gcgcggcgtt cccgcagcag ttccgggaag acctcggaat
     4981 ggggggtgag cacggtgttc ccggtcagcg gcaccctgtg cagccggccg agcacccggc
     5041 cgagttcgcg ggccagggcg agcagcgcgt tccggtcggt cgtgccgtcc atcgcggacc
     5101 gccaggtggt gccggtcatc cggctcatca ccaggtaggg ccacggccag gctccggtgc
     5161 cgggccgcag ctcgccgcgg ccgaggaggc ggggcaccgg caccggggcg tccgccagga
     5221 ccgcgtacgc ctccgactcc gacgcgaggc tctccggacc gcaccagtgc tcgccgaaca
     5281 gcttgatcac cgggtcgggc tcgccgacca gtacggggtt ggtgctctcg ccgggcaccc
     5341 gcagcaccgg cggcaccggc agcccgagct cctccagggc tcggcgggcc agcggctccc
     5401 agaattcctg gtcgttccgc aggctcgcgt aggaatcatc cgaatcaata cggtcgagaa
     5461 gtaacaggga ttcttgtgtc acagcggacc tctattcaca gggtacgggc cggcttaatt
     5521 ccgcacggcc ggtcgcgaca cggcctgtcc gcaccgcgga tcaggcgttg acgatgacgg
     5581 gctggtcggc cacgtcgggg acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     6781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     6841 accatgatta ccagatctgg ctcgagctcc accgcggtgg cggccgct
//
