pSK5632 vector (V003158)

LOCUS       V003158                 5920 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003158
VERSION     V003158
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Grkovic S, Brown MH, Hardie KM, Firth N, Skurray RA.
  TITLE     Stable low-copy-number Staphylococcus aureus shuttle vectors
  JOURNAL   Microbiology (Reading, Engl.) 149 (PT 3), 785-794 (2003)
   PUBMED   12634346
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Grkovic S, Brown MH, Hardie KM, Firth N, Skurray RA.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2002) Biological Sciences, University of Sydney,
            Macleay Building, A12, Sydney, NSW 2006, Australia
REFERENCE   3  (bases 1 to 5920)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5920)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Microbiology (Reading, Engl.) 149 (PT 3), 785-794 (2003)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-NOV-2002) Biological Sciences, University of Sydney, Macleay
            Building, A12, Sydney, NSW 2006, Australia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..57)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(58..74)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             700..1347
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(1605..2342)
                     /codon_start=1
                     /gene="par"
                     /product="partitioning protein"
                     /label="par"
                     /note="Par"
                     /protein_id="AAO24786.1"
                     /translation="MKTIKMVADELNVTKQTVVNNAKNLNISFEKENGVNYIDDNDYLK
                     IVEKITKKERTTQNKENKKSEITYENTEKNRYNNSDGFETLKTKVNELEKQVEIFETRA
                     KNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILALESNKKIQKLEHQLEEERQLSYSF
                     DKSTNDRENFDVQEASYTSDSVNTDQYQKEEKKPEVQPKDISESQQDEKSQQQDDSFNQ
                     NDKDIAIEETQTKKGFWSRLFGG"
     gene            complement(1605..2342)
                     /gene="par"
                     /label="par"
     CDS             2767..3687
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label="rep"
                     /note="Rep"
                     /protein_id="AAO24787.1"
                     /translation="MSKFKKISASEFETLRFYQLPKFLFEDEYFSKMPTDAKVMYALLK
                     DRFELSRLNNWVDSENNIYLLYTNKQLCSILNYAEPKIIKLKKELEKYNLIINERQGLN
                     KPNKIYLLEPTYDKELINSKFQNKEFISSRTNKSSVQELINSKSSDTDFNNTEYIETKN
                     NDTNYTNDTSNMISKNSHSNHTNHQQTEFNNDALKFQALEELPSQIKSYVSNFEIKDIR
                     IIKSILLKGKKSFNNTHDTYYRLEDVEFELVSVLKRFKAMLLQKNETVETMQGYLMQSI
                     KAELEEIHALNMRRQNIPQYNIFNQ"
     gene            2767..3687
                     /gene="rep"
                     /label="rep"
     promoter        3782..3886
                     /label="AmpR promoter"
     CDS             3887..4744
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4918..5506
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5794..5815
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5830..5860
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5868..5884
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5892..5908
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"