LOCUS       V003158                 5920 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003158
VERSION     V003158
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Grkovic S, Brown MH, Hardie KM, Firth N, Skurray RA.
  TITLE     Stable low-copy-number Staphylococcus aureus shuttle vectors
  JOURNAL   Microbiology (Reading, Engl.) 149 (PT 3), 785-794 (2003)
   PUBMED   12634346
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Grkovic S, Brown MH, Hardie KM, Firth N, Skurray RA.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2002) Biological Sciences, University of Sydney,
            Macleay Building, A12, Sydney, NSW 2006, Australia
REFERENCE   3  (bases 1 to 5920)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5920)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Microbiology (Reading, Engl.) 149 (PT 3), 785-794 (2003)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-NOV-2002) Biological Sciences, University of Sydney, Macleay
            Building, A12, Sydney, NSW 2006, Australia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..57)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(58..74)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             700..1347
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(1605..2342)
                     /codon_start=1
                     /gene="par"
                     /product="partitioning protein"
                     /label="par"
                     /note="Par"
                     /protein_id="AAO24786.1"
                     /translation="MKTIKMVADELNVTKQTVVNNAKNLNISFEKENGVNYIDDNDYLK
                     IVEKITKKERTTQNKENKKSEITYENTEKNRYNNSDGFETLKTKVNELEKQVEIFETRA
                     KNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILALESNKKIQKLEHQLEEERQLSYSF
                     DKSTNDRENFDVQEASYTSDSVNTDQYQKEEKKPEVQPKDISESQQDEKSQQQDDSFNQ
                     NDKDIAIEETQTKKGFWSRLFGG"
     gene            complement(1605..2342)
                     /gene="par"
                     /label="par"
     CDS             2767..3687
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label="rep"
                     /note="Rep"
                     /protein_id="AAO24787.1"
                     /translation="MSKFKKISASEFETLRFYQLPKFLFEDEYFSKMPTDAKVMYALLK
                     DRFELSRLNNWVDSENNIYLLYTNKQLCSILNYAEPKIIKLKKELEKYNLIINERQGLN
                     KPNKIYLLEPTYDKELINSKFQNKEFISSRTNKSSVQELINSKSSDTDFNNTEYIETKN
                     NDTNYTNDTSNMISKNSHSNHTNHQQTEFNNDALKFQALEELPSQIKSYVSNFEIKDIR
                     IIKSILLKGKKSFNNTHDTYYRLEDVEFELVSVLKRFKAMLLQKNETVETMQGYLMQSI
                     KAELEEIHALNMRRQNIPQYNIFNQ"
     gene            2767..3687
                     /gene="rep"
                     /label="rep"
     promoter        3782..3886
                     /label="AmpR promoter"
     CDS             3887..4744
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4918..5506
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5794..5815
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5830..5860
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5868..5884
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5892..5908
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcact
       61 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      121 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      181 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
      241 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
      301 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
      361 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
      421 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
      481 tttataggtt aatgtcatga taataatggt ttcttagacg tcttcaacta aagcacccat
      541 tagttcaaca aacgaaaatt ggataaagtg ggatattttt aaaatatata tttatgttac
      601 agtaatattg acttttaaaa aaggattgat tctaatgaag aaagcagaca agtaagcctc
      661 ctaaattcac tttagataaa aatttaggag gcatatcaaa tgaactttaa taaaattgat
      721 ttagacaatt ggaagagaaa agagatattt aatcattatt tgaaccaaca aacgactttt
      781 agtataacca cagaaattga tattagtgtt ttataccgaa acataaaaca agaaggatat
      841 aaattttacc ctgcatttat tttcttagtg acaagggtga taaactcaaa tacagctttt
      901 agaactggtt acaatagcga cggagagtta ggttattggg ataagttaga gccactttat
      961 acaatttttg atggtgtatc taaaacattc tctggtattt ggactcctgt aaagaatgac
     1021 ttcaaagagt tttatgattt atacctttct gatgtagaga aatataatgg ttcggggaaa
     1081 ttgtttccca aaacacctat acctgaaaat gctttttctc tttctattat tccatggact
     1141 tcatttactg ggtttaactt aaatatcaat aataatagta attaccttct acccattatt
     1201 acagcaggaa aattcattaa taaaggtaat tcaatatatt taccgctatc tttacaggta
     1261 catcattctg tttgtgatgg ttatcatgca ggattgttta tgaactctat tcaggaattg
     1321 tcagataggc ctaatgactg gcttttataa tatgagataa tgccgactgt actttttaca
     1381 gtcggttttc taatgtcact aacctgcccc gttagttgaa gaaggttttt atattacagc
     1441 tccagatcca tatccttcag atccctagat aattcttctg ataatttagt ttttgttaga
     1501 gtttaacata actgatgatt atcagaagtt tctttaatat aaataaaagc actataagcg
     1561 acttaactta tagtgcttat ttaatatatt ttttcaaaca atcattagcc accaaacaaa
     1621 cgactccaga agcctttttt agtttgggtt tcttctatag caatatcttt atcattttga
     1681 ttaaatgaat catcttgttg ttgtgatttt tcatcttgtt gactctcaga tatatcttta
     1741 ggttgaacct ctggtttctt ttcttctttc tgatattgat cagtatttac agaatccgaa
     1801 gtatatgatg cttcttgtac atcaaagttt tctcgatcat tagtagattt atcaaaggaa
     1861 taacttaatt gtctctcttc ctctaattga tgttccaatt tttgaatttt tttattgctt
     1921 tctaaagcta aaatttgttg attctctaat aatttattta atgtattaac attgctattt
     1981 tgttggtcta gttgtttagt taaattctca atatactttt catcattttt agctctagtt
     2041 tcaaagattt ctacctgctt ttctaattcg tttacttttg tttttaatgt ctcaaagcca
     2101 tctgaattat tatatctatt cttttcagtg ttttcataag ttatttctga ttttttattt
     2161 tctttatttt gagttgttct ttctttctta gtgatttttt ctactatttt caaataatca
     2221 ttatcatcaa tataattcac tccattttct ttttcaaaag atatattcaa atttttagca
     2281 ttattaacaa cagtttgttt agttacattc aattcatcag caaccatttt tatagttttc
     2341 atatcatcca cccttaggca cctaatttac tacctaattt taccacctaa caatttttct
     2401 ttcatctttt ttaccgccta aatatatcat ttttactacc taagtaccta atttactacc
     2461 taattagcca ctaaaataat aaattttgtt gttttaaggt cataacatga ttctgatctg
     2521 tacttaaaac gctttatata cacctttaaa aatgttaata ttatcttatg aattttaaaa
     2581 agccaatgct attggcgtag catcggctct ggtaattaaa acgatttgcg ttcgtttatt
     2641 tatatatttt tttgatactt gtattatata tatctactcg tctaagtgca agcacaaaac
     2701 atataactta cgtaaaaatt gttttattac ctcaactcta agtaaaagga aatgaggttt
     2761 tttattatgt ctaaatttaa aaaaatatct gcaagtgaat tcgaaacatt acgtttttat
     2821 caattaccca aatttttatt tgaagatgaa tacttttcta aaatgcccac agatgcaaaa
     2881 gttatgtatg ctttattaaa agatcgcttt gaactatcaa gattaaataa ctgggtagat
     2941 tcagaaaata atatttatct tttatatacc aataaacagt tatgctcaat tttaaattat
     3001 gcagaaccaa aaatcattaa attaaaaaaa gaattagaaa aatacaattt gattataaac
     3061 gaaagacaag gtttaaataa acctaacaaa atttatttac tcgaacccac atatgacaag
     3121 gaactaataa attctaagtt ccagaacaaa gaatttatta gttccagaac taataaatca
     3181 tcagttcaag aactaataaa ttctaagtca agtgatactg attttaataa tactgaatat
     3241 atagagacta agaataatga tacgaattat acgaatgata catctaacat gatttctaag
     3301 aattctcatt cgaatcatac aaatcatcaa caaaccgaat ttaataatga tgccttaaaa
     3361 ttccaggcgc ttgaagaatt accttcgcaa atcaaatctt atgtaagtaa ttttgaaatt
     3421 aaagacatcc gtattattaa aagtatctta ctcaagggga aaaagtcatt taataataca
     3481 catgatacat attaccgttt agaagacgtc gaatttgaac ttgtaagtgt tttaaaacgt
     3541 tttaaagcca tgttgctaca aaaaaatgaa accgttgaaa ctatgcaagg ctatttaatg
     3601 caatcaatta aagctgaact tgaagaaata catgcattaa atatgcgtcg tcaaaacata
     3661 cctcaataca atatctttaa tcaataactc aaataatctt acaacaatca aaacaacatc
     3721 aaaatttgga attaagtcaa cagaaaagga tctcccaggt ggcacttttc ggggaaatgt
     3781 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3841 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3901 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3961 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4021 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     4081 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4141 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4201 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     4261 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     4321 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     4381 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     4441 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4501 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4561 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4621 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4681 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4741 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4801 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4861 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4921 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4981 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5041 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     5101 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5161 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5221 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5281 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     5341 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5401 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5461 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5521 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5581 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     5641 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     5701 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     5761 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     5821 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     5881 acaatttcac acaggaaaca gctatgacca tgattacgcc
//
