pSJS1472 vector (V003169)

LOCUS       V003169                 9582 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003169
VERSION     V003169
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9582)
  AUTHORS   Renzette N, Gumlaw N, Nordman JT, Krieger M, Yeh SP, Long E, Centore
            R, Boonsombat R, Sandler SJ.
  TITLE     Localization of RecA in Escherichia coli K-12 using RecA-GFP
  JOURNAL   Mol. Microbiol. 57 (4), 1074-1085 (2005)
   PUBMED   16091045
REFERENCE   2  (bases 1 to 9582)
  AUTHORS   Gumlaw N, Nordman JT, Sandler SJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2005) Microbiology, Universtiy of
            Massachusetts-Amherst, 639 North Pleasant Street, Amherst, MA 01003,
            USA
REFERENCE   3  (bases 1 to 9582)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9582)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
            Microbiol."; date: "2005"; volume: "57"; issue: "4"; pages:
            "1074-1085"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-MAR-2005) Microbiology, Universtiy of Massachusetts-Amherst, 639
            North Pleasant Street, Amherst, MA 01003, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             1785..2591
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             2674..3732
                     /label="RecA"
                     /note="DNA repair protein from E. coli"
     CDS             3739..4452
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     CDS             4524..5021
                     /gene="recX"
                     /label="Regulatory protein RecX"
                     /note="Regulatory protein RecX from Escherichia coli
                     (strain K12). Accession#: P33596"
     CDS             7136..7324
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    7429..7569
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7755..8343)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8517..9374)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9375..9479)
                     /label="AmpR promoter"