LOCUS       V003169                 9582 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003169
VERSION     V003169
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9582)
  AUTHORS   Renzette N, Gumlaw N, Nordman JT, Krieger M, Yeh SP, Long E, Centore
            R, Boonsombat R, Sandler SJ.
  TITLE     Localization of RecA in Escherichia coli K-12 using RecA-GFP
  JOURNAL   Mol. Microbiol. 57 (4), 1074-1085 (2005)
   PUBMED   16091045
REFERENCE   2  (bases 1 to 9582)
  AUTHORS   Gumlaw N, Nordman JT, Sandler SJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2005) Microbiology, Universtiy of
            Massachusetts-Amherst, 639 North Pleasant Street, Amherst, MA 01003,
            USA
REFERENCE   3  (bases 1 to 9582)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9582)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
            Microbiol."; date: "2005"; volume: "57"; issue: "4"; pages:
            "1074-1085"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-MAR-2005) Microbiology, Universtiy of Massachusetts-Amherst, 639
            North Pleasant Street, Amherst, MA 01003, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             1785..2591
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             2674..3732
                     /label="RecA"
                     /note="DNA repair protein from E. coli"
     CDS             3739..4452
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     CDS             4524..5021
                     /gene="recX"
                     /label="Regulatory protein RecX"
                     /note="Regulatory protein RecX from Escherichia coli
                     (strain K12). Accession#: P33596"
     CDS             7136..7324
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    7429..7569
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7755..8343)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8517..9374)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9375..9479)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
      361 cacacccgtc ctgtggatcc ggttgatgcg atcggtagcg tggcgaacta tttcaaagcg
      421 cacggctggg tgaaaggcga tcaggtcgcg gtaatggcaa acggtcaggc tccaggcttg
      481 ccaaatggct tcaaaactaa gtacagcatt tcgcagcttg ccgccgcagg tttaacgcca
      541 cagcagccgc tgggcaacca tcaacaagcc agcctgctgc gtctggatgt tggcaccggc
      601 taccagtact ggtacggtct gccgaacttc tacaccatca cccgttacaa ccacagcacc
      661 cattacgcaa tggcggtctg gcagttagga caagccgtgg cgctggcgcg agtacagtag
      721 ctatttacaa gaggggggcg tgagcttccc tctggctaat cagctttttt ctgaatcctc
      781 ctcgtaaaat tgcaacgcca acaccatctt cctgacgaaa gtgctatctt gtccggcata
      841 aatttttgac tgacagaggt tgtgatgact gacagtgaac tgatgcagtt aagtgaacag
      901 gttgggcagg cgctgaaagc ccgtggcgca accgtaacaa ctgccgagtc ttgtaccggt
      961 ggttgggtag cgaaagtgat taccgatatt gccggtagct ccgcctggtt tgaacgcgga
     1021 tttgtcacct acagtaacga agccaaagcg cagatgatcg gcgtacgggg ggggggggcg
     1081 ctgaggtctg cctcgtgaag aaggtgttgc tgactcatac caggcctgaa tcgccccatc
     1141 atccagccag aaagtgaggg agccacggtt gatgagagct ttgttgtagg tggaccagtt
     1201 ggtgattttg aacttttgct ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat
     1261 ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccgccgtc ccgtcaagtc
     1321 agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg
     1381 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     1441 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     1501 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     1561 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     1621 ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     1681 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     1741 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgagc catattcaac
     1801 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     1861 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
     1921 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     1981 agatggtcag actaaactgg ctgacggaat ttatgccact tccgaccatc aagcatttta
     2041 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcgttcc
     2101 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     2161 tgcgccggtt gcactcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     2221 gcctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     2281 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     2341 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     2401 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     2461 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     2521 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     2581 atgagttttt ctaatactgt atgagcatac agtataattg cttcaacaga acatattgac
     2641 tatccggtat tacccggcat gacaggagta aaaatggcta tcgacgaaaa caaacagaaa
     2701 gcgttggcgg cagcactggg ccagattgag aaacaatttg gtaaaggctc catcatgcgc
     2761 ctgggtgaag accgttccat ggatgtggaa accatctcta ccggttcgct ttcactggat
     2821 atcgcgcttg gggcaggtgg tctgccgatg ggccgtatcg tcgaaatcta cggaccggaa
     2881 tcttccggta aaaccacgct gacgctgcag gtgatcgccg cagcgcagcg tgaaggtaaa
     2941 acctgtgcgt ttatcgatgc tgaacacgcg ctggacccaa tctacgcacg taaactgggc
     3001 gtcgatatcg agaacctgct gtgctcccag ccggacaccg gcgagcaggc actggaaatc
     3061 tgtgacgccc tggcgcgttc tggcgcagta gacgttatcg tcgttgactc cgtggcggca
     3121 ctgacgccga aagcggaaat cgaaggcgaa atcggcgact ctcacatggg ccttgcggca
     3181 cgtatgatga gccaggcgat gcgtaagctg gcgggtaacc tgaagcagtc caacacgctg
     3241 ctgatcttca tcaaccagat ccgtatgaaa attggtgtga tgttcggtaa cccggaaacc
     3301 actaccggtg gtaacgcgct gaaattctac gcctctgttc gtctcgacat ccgtcgtatc
     3361 ggcgcggtga aagagggcga aaacgtggtg ggtagcgaaa cccgcgtgaa agtggtgaag
     3421 aacaaaatcg ctgcgccgtt taaacaggct gaattccaga tcctctacgg cgaaggtatc
     3481 aacttctacg gcgaactggt tgacctgggc gtaaaagaga agctgatcga gaaagcaggc
     3541 gcgtggtaca gctacaaagg tgagaagatc ggtcagggta aagcgaatgc gactgcctgg
     3601 ctgaaagata acccggaaac cgcgaaagag atcgagaaga aagtacgtga gttgctgctg
     3661 agcaacccga actcaacgcc ggatttctct gtagatgata gcgaaggcgt agcagaaact
     3721 aacgaagatt ttgggagcat cagtaaaaga gaagaacttt tcactggagt tgtcccaatt
     3781 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
     3841 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
     3901 gttccatggc caacacttgt cactactttc gcgtatggtc ttcaatgctt tgcgagatac
     3961 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
     4021 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
     4081 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
     4141 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
     4201 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
     4261 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     4321 ttaccagaca accattacct gtccacacaa tctacccttt cgaaagatcc caacgaaaag
     4381 cgtgaccaca tggtccttct tgagtttgta actgctgctg ggattacaca tggcatggat
     4441 gagctctaca aataatcgtc ttgtttgata cacaagggtc gcatctgcgg cccttttgct
     4501 tttttaagtt gtaaggatat gccatgacag aatcaacatc ccgtcgcccg gcatatgctc
     4561 gcctgttgga tcgtgcggta cgcattctgg cggtgcgcga tcacagtgag caagaactgc
     4621 gacgtaaact cgcggcaccg attatgggca aaaatggccc agaagagatt gatgctacgg
     4681 cagaagatta cgagcgcgtt attgcctggt gccatgaaca tggctatctc gatgacagcc
     4741 gatttgttgc gcgctttatc gccagccgta gccgcaaagg ttatggacct gcgcgtattc
     4801 gccaggaact gaatcagaaa ggtatttccc gcgaagcgac agaaaaagcg atgcgtgaat
     4861 gtgacatcga ctggtgcgca ctggcgcgcg atcaggcgac gcgaaaatat ggcgaacctt
     4921 tgccaactgt cttttcagaa aaagttaaga tccagcgttt tctgctctat cgtggctatc
     4981 tgatggaaga tatccaggag atttggcgaa attttgccga ctgaacgcat acggtatttt
     5041 acttcccagt caagaaaact tatcttattc ccacttttca gttaccagcc cggcggttaa
     5101 gacacgctgg agctggtggc gatatttcgt tagcttgatt tcaggataat tatgagcaag
     5161 agcaccgctg agatccgtca ggcgtttctc gactttttcc atagtaaggg acatcaggta
     5221 gttgccagca gctccctggt accccataac gacccaactt tgttgtttac caacgccggg
     5281 atgaaccagt tcaaggatgt gttccttggg ctcgacaagc gtaattattc ccgcgctacc
     5341 acttcccaac gctgcgtgcg tgcgggtggt aaacacaacg acctggaaaa cgtcggttac
     5401 accgcgcgtc accatacctt cttcgaaatg ctgggcaact tcagcttcgg cgactatttc
     5461 aaacacgatg ccattcagtt tgcatgggaa ctgctgacca gcgaaaaatg gtttgccctg
     5521 ccgaaagagc gtctgtgggt taccgtctat gaaagcgacg acgaagccta cgaaatctgg
     5581 gaaaaagaag tagggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     5641 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     5701 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     5761 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     5821 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg
     5881 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     5941 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     6001 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt
     6061 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa
     6121 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc
     6181 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
     6241 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
     6301 cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg
     6361 ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt
     6421 gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg
     6481 gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa
     6541 ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca
     6601 tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc agcgttgggt
     6661 cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg
     6721 gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct
     6781 gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt
     6841 aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag
     6901 gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac
     6961 cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac
     7021 gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt
     7081 tcatcggtat cattaccccc atgaacagaa attccccctt acacggaggc atcaagtgac
     7141 caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct
     7201 tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca
     7261 cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa
     7321 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     7381 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     7441 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     7501 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     7561 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7621 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7681 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7741 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7801 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7861 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7921 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7981 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8041 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8101 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8161 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     8221 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8281 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8341 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8401 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8461 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8521 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8581 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8641 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8701 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8761 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8821 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8881 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8941 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9001 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9061 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9121 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9181 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9241 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9301 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9361 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9421 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9481 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     9541 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aa
//
