Basic Vector Information
- Vector Name:
- pP3CG
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7740 bp
- Type:
- Reporter vector
- Replication origin:
- ori
- Source/Author:
- Blazso P.
pP3CG vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pP3CG vector Sequence
LOCUS 40924_34012 7740 bp DNA circular SYN 18-DEC-2018
DEFINITION Reporter vector pP3CG, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7740)
AUTHORS Blazso P.
TITLE pP3CG, 3.6kb mouse cyclin C promoter - hrGFP reporter construction
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7740)
AUTHORS Blazso P.
TITLE Direct Submission
JOURNAL Submitted (07-JAN-2011) Institute of Genetics, Biological Research
Center, Hungarian Academy of Sciences, Temesvari krt. 62, Szeged
H6726, Hungary
REFERENCE 3 (bases 1 to 7740)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7740)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(07-JAN-2011) Institute of Genetics, Biological Research Center,
Hungarian Academy of Sciences, Temesvari krt. 62, Szeged H6726,
Hungary"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7740
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 25..129
/label=AmpR promoter
CDS 130..987
/label=AmpR
/note="beta-lactamase"
rep_origin 1161..1749
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2037..2058
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2073..2103
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2111..2127
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2135..2151
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 2172..2190
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
regulatory 2221..5801
/label=3.6 kb promoter fragment of mouse cyclin C gene
/note="3.6 kb promoter fragment of mouse cyclin C gene"
/regulatory_class="promoter"
CDS 5810..6526
/label=hrGFP
/note="humanized Renilla green fluorescent protein"
primer_bind complement(6553..6569)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(6608..6626)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 6900..7021
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7056..7072)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(7098..7116)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(7126..7142)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 7284..7739
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
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