LOCUS       40924_34012        7740 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pP3CG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Blazso P.
  TITLE     pP3CG, 3.6kb mouse cyclin C promoter - hrGFP reporter construction
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Blazso P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2011) Institute of Genetics, Biological Research 
            Center, Hungarian Academy of Sciences, Temesvari krt. 62, Szeged 
            H6726, Hungary
REFERENCE   3  (bases 1 to 7740)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7740)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-JAN-2011) Institute of Genetics, Biological Research Center, 
            Hungarian Academy of Sciences, Temesvari krt. 62, Szeged H6726, 
            Hungary"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        25..129
                     /label=AmpR promoter
     CDS             130..987
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1161..1749
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2037..2058
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2073..2103
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2111..2127
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2135..2151
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2172..2190
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2221..5801
                     /label=3.6 kb promoter fragment of mouse cyclin C gene
                     /note="3.6 kb promoter fragment of mouse cyclin C gene"
                     /regulatory_class="promoter"
     CDS             5810..6526
                     /label=hrGFP
                     /note="humanized Renilla green fluorescent protein"
     primer_bind     complement(6553..6569)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6608..6626)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    6900..7021
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7056..7072)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(7098..7116)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7126..7142)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7284..7739
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
       61 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      121 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      181 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
      241 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
      301 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
      361 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
      421 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
      481 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
      541 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
      601 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
      661 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
      721 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
      781 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
      841 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
      901 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
      961 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     1021 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1081 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1141 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1201 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1261 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     1321 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1381 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1441 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1501 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1561 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1621 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1681 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1741 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1801 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     1861 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     1921 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     1981 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     2041 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     2101 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     2161 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc
     2221 ggccgcaaaa atatccccaa accccacaaa tatcagtatt gctatgccta ttaggtgtta
     2281 caaagatgtg ggaaagtgta taggcacaga cataaagtag atgctaggtg ctaagataac
     2341 aatagacatg tataatatat aaattaatag agtacataga agaggcctgc aaccttagca
     2401 cttagtaggt gcaggcagga ggatcagaaa ggtaaagtag aacattaatg gtatcatact
     2461 tgtcaacttg acacaaacct agatgtccct tcaaagagga ctctcagttg agtaattacc
     2521 ttcattagac tggcctgtgg gcttgtctgt gaggcatttt cttgattgct aattgatatg
     2581 ggaggagcca gtccactgtg ggcagtgcca tccctgagca ggtgggctca tgaaacataa
     2641 caaagtagta agccacggtg aattaagatt ttctccatgg tctctgcttc agttttgaag
     2701 cagattcccg ccatggcctt cctcgatgct ggagcagaag gtgaagccaa ccctttatat
     2761 acccaagttg cctttgtcag cattttatca caataacaga gaagcaaatc agaacacttg
     2821 agttcacagt gagttccagg ccagcccagg atactgcaac cctctccccc aagacagagt
     2881 ttctctgtgt agctttggcc attctttttt tttttttttt ttttttaaag atttatttat
     2941 ttattacatg taagtacact gtagctgtct tcagacacac cagaagaggg cgtcagattt
     3001 cgctacagat ggttgtgagc caccatgtgg ttgctgggat ttgaactctg gacctttgga
     3061 agaacagtcg ggtgctctta cccactgagc catctcacca gcccagcttt ggccattctt
     3121 gaactcattc tgtagaccag gctggcctca aactcaagag atcagcctgc ctctgcctct
     3181 gcctctgcct ctgcctcctc tgcctttgcc tctgcctcct ctgcctctgc ctctgtctcc
     3241 tctgcctctg cctctgcctc tgcctcctct gcctctgcct cctctgcctc ctctgcctcc
     3301 tctgcctcct ctgcctcctc tgcctcctct gcctctgcct ctgcctctgc ctctgcctct
     3361 gcctctgcct ctgcctctgc ctctgcctct gcctcctgcg ttctgggatt aaagatgtgt
     3421 gctaacgcct tcccgttttt aaagtgttat aggagctagg tgtagtggct cacactttta
     3481 gttccagcac tcaggagaaa gaggcaggca gatctttgtg agtttaagga cagccaaagg
     3541 tacatagtga gatcttgtct aaaacagtaa aaaaaaaaga aagaaagaaa gaaagaaaaa
     3601 agtgtcatag aagcacataa aacatcgcaa gcagtttgtt acagaatctt tatgcaaggc
     3661 tgataaaaca aaaggaaaag agcagagaga attaaagcaa atctaaatct ctatgatgca
     3721 caacttgggt gatggttgtg tctttaaaac acattttgaa aaatatgatc tgaggggctg
     3781 gagagatggc tcagcggtta agagcactga ctgctcttcc agagatcctg agttcaattc
     3841 ccagcaacca catggttgtt cacaaccatc tgtaatggaa cctgatgccc tcttctggtg
     3901 tgtctgaaga cagctacagg tgtactcata taaataaaat aaataaatct ttaaaaaaaa
     3961 gaaaaagaaa aatatgatct gccgggcgtg gtggcacacg cctttaatcc cagcactcgg
     4021 gaggcaaagg caggcggatt tctgagttcc aggccagcct ggtctacaaa gtgagttcca
     4081 ggacaaccag ggctatgcag agaaaccctg tcttgaaaaa acaaaacaaa caaacaaaca
     4141 aactgatctg cagtgaaaca aacagtaatg ttcacaaatg tattacatgc ctctcaacat
     4201 tttttaggta ccttggctat taagttggag tcatttgggt atattctggt cagtgggtca
     4261 tgaaaattga ttgggttgtt tctatttcaa gactcttaat actagttatt aaagagctcg
     4321 cttgagtcat gttctgccca gaaagatgtt gatcttggaa gcaaggaaca ttgaaatgaa
     4381 tagttgggag atagaaaccc tctagttctc tgaagatcta gagggcagcc aggaagaact
     4441 agccagcttt aggaaatacc atgagaatga gacgaatttt caagctaagt tgggggtata
     4501 acaatgttat aacaatgaag cctagtctag ctcatccaaa ttcaagaata aatgtatttc
     4561 acaggttaat tgtgtacgtg cctaagtggg tggaatttaa aaacatcttt tctatagttg
     4621 gcatactagt ttcacttata gaaattgttt tcactccatt ccacatctca attagactcc
     4681 actcacaaag gaaaaacatt ggtgctatct tcctcggaat tcttgactaa ttttcaggtg
     4741 tgcccaatca tttagagaca ttttttaatt taaattaaaa cttaaaagat ttagatagat
     4801 cctcacctgt cctaggctgg cccaaaacaa cgtatctgag ggtattgaat acccttagct
     4861 tgcaggtgta tatagtgctg agtatcaccc agagcttctg gggcaccagc aagcatttta
     4921 catccttgat tatgtacatc ccttttcctc ttttccccta gatggcgtct cagcatgaag
     4981 caaggactgg cctgaaactc ctcatgttct tgactctgcc gccgaagtac agtacgaaac
     5041 gcgaacgcaa agttgcaatg ataacagcaa catttaaata ttatttaatt gcatttctgc
     5101 ggcataccca tttggaacgt aagtgttagc tagatggtac atttatatgc tcatgttttt
     5161 aggtaaacaa gtagccggct ctgacataaa aagattgcta ctctaagtta agtctacact
     5221 taactcgtta ggcttaatac cgacaggaac ttttactaag agcacttaca tcttaaggcc
     5281 aaaattactt tcatttattt ctcctttcag ccctagggaa aaagcagtca tatactgcca
     5341 tgctttcttc aacgccagtg tgctatttct gaaggcctac accgtggatt aacaagatcc
     5401 ctaaatggac ccaaaagaaa accgcactag accagaaacc cagtgtgtgg acgctctgga
     5461 ggagaattgg caggaaacct cggggagagc ctctcgccac cgggagcctc cgcccgtgtt
     5521 tccttagctc ccgcctcaac aagcccattg gcggtcgggc ggttgctagc ttcctccgca
     5581 actagggcgg gccggaactt ttgtctgccg gaaccggtgt acgccgctca gtgttgtcga
     5641 ccggcgtcaa ggcgacgagg gtgctgttct cttcctccgg ctgcggtggc tgtggagttc
     5701 agcccagcca gtctggtagg agcacagtcg tccggactgt gtctatggtc gctccgcggg
     5761 ccgctttgct ggctggtgca tttgtatcag ggcaagcagt ggatccacca tggtgagcaa
     5821 gcagatcctg aagaacaccg gcctgcagga gatcatgagc ttcaaggtga acctggaggg
     5881 cgtggtgaac aaccacgtgt tcaccatgga gggctgcggc aagggcaaca tcctgttcgg
     5941 caaccagctg gtgcagatcc gcgtgaccaa gggcgccccc ctgcccttcg ccttcgacat
     6001 cctgagcccc gccttccagt acggcaaccg caccttcacc aagtaccccg aggacatcag
     6061 cgacttcttc atccagagct tccccgccgg cttcgtgtac gagcgcaccc tgcgctacga
     6121 ggacggcggc ctggtggaga tccgcagcga catcaacctg atcgaggaga tgttcgtgta
     6181 ccgcgtggag tacaagggcc gcaacttccc caacgacggc cccgtgatga agaagaccat
     6241 caccggcctg cagcccagct tcgaggtggt gtacatgaac gacggcgtgc tggtgggcca
     6301 ggtgatcctg gtgtaccgcc tgaacagcgg caagttctac agctgccaca tgcgcaccct
     6361 gatgaagagc aagggcgtgg tgaaggactt ccccgagtac cacttcatcc agcaccgcct
     6421 ggagaagacc tacgtggagg acggcggctt cgtggagcag cacgagaccg ccatcgccca
     6481 gctgaccagc ctgggcaagc ccctgggcag cctgcacgag tgggtgtaat aggaattcga
     6541 tatcaagctt atcgataccg tcgacctcga gggggggccc ggtaccaggt aagtgtaccc
     6601 aattcgccct atagtgagtc gtattacaat tcactcgatc gcccttccca acagttgcgc
     6661 agcctgaatg gcgaatggag atccaatttt taagtgtata atgtgttaaa ctactgattc
     6721 taattgtttg tgtattttag attcacagtc ccaaggctca tttcaggccc ctcagtcctc
     6781 acagtctgtt catgatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     6841 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     6901 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6961 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7021 aacgcgtaaa ttgtaagcgt taatatcaag cttatcgata ccgtcgacct cgaggggggg
     7081 cccggtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     7141 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     7201 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     7261 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     7321 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7381 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7441 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7501 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7561 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7621 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7681 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
//
