Basic Vector Information
- Vector Name:
- pORI101
- Length:
- 5316 bp
- Type:
- Shuttle vector
- Replication origin:
- ori
- Source/Author:
- Bourn WR, Jansen Y, Stutz H, Warren RM, Williamson AL, van Helden PD.
pORI101 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pORI101 vector Sequence
LOCUS V004211 5316 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004211
VERSION V004211
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 5316)
AUTHORS Bourn WR, Jansen Y, Stutz H, Warren RM, Williamson AL, van Helden
PD.
TITLE Creation and characterisation of a high-copy-number version of the
pAL5000 mycobacterial replicon
JOURNAL Tuberculosis (Edinb) 87 (6), 481-488 (2007)
PUBMED 17888739
REFERENCE 2 (bases 1 to 5316)
AUTHORS Bourn WR.
TITLE Direct Submission
JOURNAL Submitted (10-JAN-2007) Medical Virology and Institute of Infectious
Disease and Molecular Medicine, University of Cape Town, Anzio Road
Observatory, Cape Town 7925, South Africa
REFERENCE 3 (bases 1 to 5316)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5316)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Tuberculosis (Edinb)"; date: "2007"; volume: "87"; issue: "6";
pages: "481-488"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(10-JAN-2007) Medical Virology and Institute of Infectious Disease
and Molecular Medicine, University of Cape Town, Anzio Road
Observatory, Cape Town 7925, South Africa"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5316
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 250..838
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 1126..1147
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 1162..1192
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 1200..1216
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 1224..1240
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1328..1344)
/label="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 1374..3961
/note="mycobacterial origin; OriM"
CDS complement(4202..5197)
/gene="hyg"
/label="Hygromycin-B 7''-O-kinase"
/note="Hygromycin-B 7''-O-kinase from Streptomyces
hygroscopicus. Accession#: P09979"
This page is informational only.