LOCUS       V004211                 5316 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004211
VERSION     V004211
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Bourn WR, Jansen Y, Stutz H, Warren RM, Williamson AL, van Helden
            PD.
  TITLE     Creation and characterisation of a high-copy-number version of the
            pAL5000 mycobacterial replicon
  JOURNAL   Tuberculosis (Edinb) 87 (6), 481-488 (2007)
   PUBMED   17888739
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Bourn WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2007) Medical Virology and Institute of Infectious
            Disease and Molecular Medicine, University of Cape Town, Anzio Road
            Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 5316)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5316)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Tuberculosis (Edinb)"; date: "2007"; volume: "87"; issue: "6";
            pages: "481-488"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-JAN-2007) Medical Virology and Institute of Infectious Disease
            and Molecular Medicine, University of Cape Town, Anzio Road
            Observatory, Cape Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      250..838
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    1126..1147
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1162..1192
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1200..1216
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1224..1240
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(1328..1344)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      1374..3961
                     /note="mycobacterial origin; OriM"
     CDS             complement(4202..5197)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
ORIGIN
        1 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
       61 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
      121 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      181 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      241 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      301 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      361 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
      421 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      481 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      541 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      601 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      661 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      721 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      781 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      841 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      901 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      961 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     1021 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     1081 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     1141 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     1201 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacc agatctggct
     1261 cgagctccac cgcggtggcg gccgctctag gatcccccgg gctgcaggaa ttcgatatca
     1321 agcttatcga taccgtcgac ctcgaggggg ggcccggtac gtacccgggc atcatcgagc
     1381 cgagaacgtt atcgaagttg gtcatgtgta atcccctcgt ttgaactttg gattaagcgt
     1441 agatacaccc ttggacaagc cagttggatt cggagacaag caaattcagc cttaaaaagg
     1501 gcgaggcctg cggtggtgga acaccgcagg gcctctaacc gctcgacgcg ctgcaccaac
     1561 cagcccgcga acggctggca gccagcgtaa ggcgcggctc atcgggcggc gttcgccacg
     1621 atgtcctgca cttcgagcca agcctcgaac acctgctggt gtgcacgact cacccggttg
     1681 ttgacaccgc gcgcggccgt gcgggctcgg tggggcggct gtgtcgccct tgccagcgtg
     1741 agtagcgcgt acctcacctc gcccaacagg tcgcacacag ccgattcgta cgccataaag
     1801 ccaggtgagc ccaccagctc cgtaagttcg ggcgctgtgt ggctcgtacc cgcgcattca
     1861 ggcggcaggg ggtctaacgg gtctaaggcg gcgtgtacgg ccgccacagc ggctctcagc
     1921 ggcccggaaa cgtcctcgaa acgacgcatg tgttcctcct ggttggtaca ggtggttggg
     1981 ggtgctcggc tgtcgctggt gttccaccac cagggctcga cgggagagcg ggggagtgtg
     2041 cagttgtggg gtggcccctc agcgaaatat ctgacttgga gctcgtgtcg gaccatacac
     2101 cggtgattaa tcgtggtcta ctaccaagcg tgagccacgt cgccgacgaa tttgagcagc
     2161 tctggctgcc gtactggccg ctggcaagcg acgatctgct cgaggggatc taccgccaaa
     2221 gccgcgcgtc ggccctaggc cgccggtaca tcgaggcgaa cccaacagcg ctggcaaacc
     2281 tgctggtcgt ggacgtagac catccagacg cagcgctccg agcgctcagc gcccgggggt
     2341 cccatccgct gcccaacgcg atcgtgggca atcgcgccaa cggccacgca cacgcagtgt
     2401 gggcactcaa cgcccctgtt ccacgcaccg aatacgcgcg gcgtaagccg ctcgcataca
     2461 tggcggcgtg cgccgaaggc cttcggcgcg ccgtcgacgg cgaccgcagt tactcaggcc
     2521 tcatgaccaa aaaccccggc cacatcgcct gggaaacgga atggctccac tcagatctct
     2581 acacactcag ccacatcgag gccgagctcg gcgcgaacat gccaccgccg cgctggcgtc
     2641 agcagaccac gtacaaagcg gctccgacgc cgctagggcg gaattgcgca ctgttcgatt
     2701 ccgtcaggtt gtgggcctat cgtcccgccc tcatgcggat ctacctgccg acccggaacg
     2761 tggacggact cggccgcgcg atctatgccg agtgccacgc gcgaaacgcc gaattcccgt
     2821 gcaacgacgt gtgtcccgga ccgctaccgg acagcgaggt ccgcgccatc gccaacagca
     2881 tttggcgttg gatcacaacc aagtcgcgca tttgggcgga cgggatcgtg gtctacgagg
     2941 ccacactcag tgcgcgccag tcggccatct cgcggaaggg cgcagcagcg cgcacggcgg
     3001 cgagcacagt tgcgcggcgc gcaaagtccg cgtcagccat ggaggcattg ctatgagcga
     3061 cggctacagc gacggctaca gcgacggcta caaccggcag ccgactgtcc gcaaaaagcg
     3121 gcgcgtgacc gccgccgaag gcgctcgaat caccggacta tccgaacgcc acgtcgtccg
     3181 gctcgtggcg caggaacgca gcgagtggct cgccgagcag gctgcacgcc gcgaacgcat
     3241 ccgcgcctat cacgacgacg agggccactc ttggccgcaa acggccaaac atttcgggct
     3301 gcatctggac accgttaagc gactcggcta tcgggcgagg aaagagcgtg cggcagaaca
     3361 ggaagcggct caaaaggccc acaacgaagc cgacaatcca ccgctgttct aacgcaattg
     3421 gggagcgggt gtcgcggggg ttccgtgggg ggttccgttg caacgggtcg gacaggtaaa
     3481 agtcctggta gacgctagtt ttctggtttg ggccatgcct gtctcgttgc gtgtttcgtt
     3541 gcgcccgttt tgaataccag ccagacgaga cggggttcta cgaatcttgg tcgataccaa
     3601 gccatttccg ctgaatatcg gggagctcac cgccagaatc ggtggttgtg gtgatgtacg
     3661 tggcgaactc cgttgtagtg cctgtggtgg catccgtggc cactctcgtt gcacggttcg
     3721 ttgtgccgtt acaggccccg ttgacagctc accgaacgta gttaaaacat gctggtcaaa
     3781 ctaggtttac caacgatacg agtcagctca tctagggcca gttctaggcg ttgttcgttg
     3841 cgcggttcgt tgcgcatgtt tcgtgtggtt gctagatggc tccgcaacca cacgcttcga
     3901 ggttgagtgc ttccagcacg ggcgcgatcc agaagaactt cgtcgtgcga ctgtcctcgt
     3961 tgatccttgc cgagctggga tggaagctcg gccgaccacc ctggaggaga tgatcgagga
     4021 tgccagggcc tttcacgccc gccgctgctg agcgtccgcc gccgggcccg caccgccgtc
     4081 ggccggcccg ctccgggctc gcagcagcgg gcttcggcgc gggcccgggg ctcccgagcg
     4141 cgggcggggc tccgggcggc cgccgggggc cgggggcggc gccgggcggc ccggggcgtc
     4201 aggcgccggg ggcggtgtcc ggcggccccc agaggaactg cgccagttcc tccggatcgg
     4261 tgaagccgga gagatccagc ggggtctcct cgaacacctc gaagtcgtgc aggaaggtga
     4321 aggcgagcag ttcgcgggcg aagtcctcgg tccgcttcca ctgcgccccg tcgagcagcg
     4381 cggccaggat ctcgcggtcg ccccggaagg cgttgagatg cagttgcacc aggctgtagc
     4441 gggagtctcc cgcatagacg tcggtgaagt cgacgatccc ggtgacctcg gtcgcggcca
     4501 ggtccacgaa gatgttggtc ccgtgcaggt cgccgtggac gaaccggggt tcgcggccgg
     4561 ccagcagcgt gtccacgtcc ggcagccagt cctccaggcg gtccagcagc cggggcgaga
     4621 ggtagcccca cccgcggtgg tcctcgacgg tcgccgcgcg gcgttcccgc agcagttccg
     4681 ggaagacctc ggaatggggg gtgagcacgg tgttcccggt cagcggcacc ctgtgcagcc
     4741 ggccgagcac ccggccgagt tcgcgggcca gggcgagcag cgcgttccgg tcggtcgtgc
     4801 cgtccatcgc ggaccgccag gtggtgccgg tcatccggct catcaccagg tagggccacg
     4861 gccaggctcc ggtgccgggc cgcagctcgc cgcggccgag gaggcggggc accggcaccg
     4921 gggcgtccgc caggaccgcg tacgcctccg actccgacgc gaggctctcc ggaccgcacc
     4981 agtgctcgcc gaacagcttg atcaccgggt cgggctcgcc gaccagtacg gggttggtgc
     5041 tctcgccggg cacccgcagc accggcggca ccggcagccc gagctcctcc agggctcggc
     5101 gggccagcgg ctcccagaat tcctggtcgt tccgcaggct cgcgtaggaa tcatccgaat
     5161 caatacggtc gagaagtaac agggattctt gtgtcacagc ggacctctat tcacagggta
     5221 cgggccggct taattccgca cggccggtcg cgacacggcc tgtccgcacc gcggtcaggc
     5281 gttgacgatg acgggctggt cggccacgtc ggggac
//
