Basic Vector Information
- Vector Name:
- pOPT-GST_tjp1aN
- Antibiotic Resistance:
- Kanamycin
- Length:
- 3734 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Fadeev A, Irion U, Nuesslein-Volhard C, Krauss J, Frohnhoefer HG.
pOPT-GST_tjp1aN vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pOPT-GST_tjp1aN vector Sequence
LOCUS 40924_33812 3734 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pOPT-GST_tjp1aN, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3734)
AUTHORS Fadeev A, Irion U, Nuesslein-Volhard C, Krauss J, Frohnhoefer HG.
TITLE Direct Submission
JOURNAL Submitted (06-APR-2015) Arbeitsgruppe Nuesslein-Volhard, Max Planck
Institute for Developmental Biology, Spemannstrasse 35, Tuebingen,
Germany 72076, Germany
REFERENCE 2 (bases 1 to 3734)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 3734)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(06-APR-2015) Arbeitsgruppe Nuesslein-Volhard, Max Planck Institute
for Developmental Biology, Spemannstrasse 35, Tuebingen, Germany
72076, Germany"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3734
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(262..1074)
/codon_start=1
/label=KanR
/note="aminoglycoside phosphotransferase"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 1196..1784
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(1970..2109)
/label=bom
/note="basis of mobility region from pBR322"
promoter 2270..2288
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
RBS 2362..2384
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 2395..3048
/codon_start=1
/label=GST
/note="glutathione S-transferase from Schistosoma
japonicum"
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 3067..3087
/codon_start=1
/label=TEV site
/note="tobacco etch virus (TEV) protease recognition and
cleavage site"
/translation="ENLYFQG"
misc_feature 3097..3552
/label=Region: tjp1a
/note="Region: tjp1a"
terminator 3656..3703
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
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