LOCUS       40924_33812        3734 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pOPT-GST_tjp1aN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Fadeev A, Irion U, Nuesslein-Volhard C, Krauss J, Frohnhoefer HG.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2015) Arbeitsgruppe Nuesslein-Volhard, Max Planck 
            Institute for Developmental Biology, Spemannstrasse 35, Tuebingen, 
            Germany 72076, Germany
REFERENCE   2  (bases 1 to 3734)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3734)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (06-APR-2015) Arbeitsgruppe Nuesslein-Volhard, Max Planck Institute 
            for Developmental Biology, Spemannstrasse 35, Tuebingen, Germany 
            72076, Germany"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(262..1074)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1196..1784
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1970..2109)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        2270..2288
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2362..2384
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2395..3048
                     /codon_start=1
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             3067..3087
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     misc_feature    3097..3552
                     /label=Region: tjp1a
                     /note="Region: tjp1a"
     terminator      3656..3703
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
       61 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      121 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      181 caataatgtc tggtaaccgc cgtcccgtca agtcagcgta atgctctgcc agtgttacaa
      241 ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
      301 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
      361 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
      421 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
      481 atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca
      541 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
      601 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
      661 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
      721 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttccctg ggatcgcagt
      781 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
      841 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
      901 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
      961 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     1021 gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcataacacc
     1081 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac
     1141 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1201 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1261 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1321 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     1381 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1441 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1501 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1561 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1621 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1681 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1741 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1801 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     1861 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     1921 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     1981 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
     2041 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     2101 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2161 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2221 tcaccgtcat caccgaaacg cgcgaggcag gatctcgatc ccgcgaaatt aatacgactc
     2281 actataggga gaccacaacg gtttccctct agtgccggct ccggagagct ccaattggaa
     2341 ttcgctagct ctagaaataa ttttgtttaa ctttaagaag gagatataca taatatgtcc
     2401 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa
     2461 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga
     2521 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat
     2581 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg
     2641 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt
     2701 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt
     2761 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat
     2821 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt
     2881 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt
     2941 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct
     3001 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggattcttct
     3061 ggttccgaaa acctgtattt ccagggctcc catatgtaca agaaggatat ctaccgaccc
     3121 aacgaaccgg tgcagataag ccaaaaccct atgcagccca actactacca ggaagacaag
     3181 ccatacagag attatgacca ccagccctac cgctatgatg gaggctacat ggaaccaaag
     3241 gctcgtaact ttgacccaca ttttgaggac agcgtgcctc catataatga tcaatggggt
     3301 cagtatgacc acacctctgg gtatgaacct caaacgctct acgaggatgg tctgaaccgc
     3361 ttgtatggtc accctcagtc acgaaacgac accggctacg acacaggtcg acctcgatat
     3421 ggcaaaccaa cctctggacc aatccgacac gatgaaccac caccatcacg cccacaatat
     3481 gacactcaac aaacctctcg ttccccagaa ccgcccaaac aacaatacta cgacccttcc
     3541 caaaggcctt cctaaggatc cagatctaag cttggtacca cgcgtgcgcg ctgatccggc
     3601 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     3661 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
     3721 agccggataa ttct
//
