Basic Vector Information
- Vector Name:
- pOGOduet
- Antibiotic Resistance:
- Tetracycline
- Length:
- 9967 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Goldbeck O, Seibold GM.
pOGOduet vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pOGOduet vector Sequence
LOCUS 40924_33732 9967 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pOGOduet, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9967)
AUTHORS Goldbeck O, Seibold GM.
TITLE Construction of pOGOduet - An inducible, bicistronic vector for
synthesis of recombinant proteins in Corynebacterium glutamicum
JOURNAL Plasmid 95, 11-15 (2018)
PUBMED 29331350
REFERENCE 2 (bases 1 to 9967)
AUTHORS Goldbeck O, Seibold GM.
TITLE Direct Submission
JOURNAL Submitted (27-NOV-2017) Institute of Microbiology and Biotechnology,
Ulm University, Albert-Einstein-Allee 11, Ulm, BW 89081, Germany
REFERENCE 3 (bases 1 to 9967)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9967)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid 95,
11-15 (2018)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(27-NOV-2017) Institute of Microbiology and Biotechnology, Ulm
University, Albert-Einstein-Allee 11, Ulm, BW 89081, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9967
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(1650..2273)
/codon_start=1
/label=TetR
/note="tetracycline repressor TetR"
/translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
protein_bind 2805..2823
/label=tet operator
/note="bacterial operator O1 for the tetR and tetA genes"
protein_bind 2839..2855
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O1 for the tetR and tetA genes"
primer_bind complement(2940..2956)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
terminator 3169..3255
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 3347..3374
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 3393..3484
/label=AmpR promoter
primer_bind complement(3805..3821)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3829..3845
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3853..3883)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(3898..3919)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(4040..5068)
/codon_start=1
/label=lacI
/note="lac repressor"
/translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ASA"
promoter complement(5069..5146)
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
promoter 5380..5408
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 5416..5432
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind complement(5536..5552)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
terminator complement(5768..5811)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 5943..5970
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
rep_origin complement(6235..6823)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 7056..7868
/codon_start=1
/label=KanR
/note="aminoglycoside phosphotransferase"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
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