LOCUS       40924_33732        9967 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pOGOduet, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Goldbeck O, Seibold GM.
  TITLE     Construction of pOGOduet - An inducible, bicistronic vector for 
            synthesis of recombinant proteins in Corynebacterium glutamicum
  JOURNAL   Plasmid 95, 11-15 (2018)
  PUBMED    29331350
REFERENCE   2  (bases 1 to 9967)
  AUTHORS   Goldbeck O, Seibold GM.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2017) Institute of Microbiology and Biotechnology,
            Ulm University, Albert-Einstein-Allee 11, Ulm, BW 89081, Germany
REFERENCE   3  (bases 1 to 9967)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9967)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid 95,
            11-15 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-NOV-2017) Institute of Microbiology and Biotechnology, Ulm 
            University, Albert-Einstein-Allee 11, Ulm, BW 89081, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1650..2273)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline repressor TetR"
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     protein_bind    2805..2823
                     /label=tet operator
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2839..2855
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     primer_bind     complement(2940..2956)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      3169..3255
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3347..3374
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        3393..3484
                     /label=AmpR promoter
     primer_bind     complement(3805..3821)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3829..3845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3853..3883)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3898..3919)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(4040..5068)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ASA"
     promoter        complement(5069..5146)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        5380..5408
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    5416..5432
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(5536..5552)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(5768..5811)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      5943..5970
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      complement(6235..6823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             7056..7868
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 gttggcactt gcaccgcaac atgctgttgt tcttggatcg tccactgtct gacgatgaac
       61 tcaaggcgtt tgaggattcc atgttttccc gctggtctgc tggtgtggtt aaggccggta
      121 tggacgcgcc actgcgtgag cacggggtca aacttgatca ggtgtctacc tggggtggag
      181 acgctgcgaa aatggcaacc tacctcgcta agggcatgtc tcaggaactg actggctccg
      241 ctactaaaac cgcgtctaag gggtcgtaca cgccgtttca gatgttggat atgttggccg
      301 atcaaagcga cgccggcgag gatatggacg ctgttttggt ggctcggtgg cgtgagtatg
      361 aggttggttc taaaaacctg cgttcgtcct ggtcacgtgg ggctaagcgt gctttgggca
      421 ttgattacat agacgctgat gtacgtcgtg aaatggaaga agaactgtac aagctcgccg
      481 gtctggaagc accggaacgg gtcgaatcaa cccgcgttgc tgttgctttg gtgaagcccg
      541 atgattggaa actgattcag tctgatttcg cggttaggca gtacgttcta gattgcgtgg
      601 ataaggctaa ggacgtggcc gctgcgcaac gtgtcgctaa tgaggtgctg gcaagtctgg
      661 gtgtggattc caccccgtgc atgatcgtta tggatgatgt ggacttggac gcggttctgc
      721 ctactcatgg ggacgctact aagcgtgatc tgaatgcggc ggtgttcgcg ggtaatgagc
      781 agactattct tcgcacccac taaaagcggc ataaaccccg ttcgatattt tgtgcgatga
      841 atttatggtc aatgtcgcgg gggcaaacta tgatgggtct tgttgttgac aatggctgat
      901 ttcatcagga atggaactgt catgctgtta tgtgcctggc tcctaatcaa agctggggac
      961 aatgggttgc cccgttgatc tgatctagtt cggattggcg gggcttcact gtatctgggg
     1021 gtggcatcgt gaatagattg cacaccgtag tgggcagtgt gcacaccata gtggccatga
     1081 gcaccaccac ccccagggac gccgacggcg cgaagctctg cgcctggtgc ggctcggaga
     1141 tcaagcaatc cggcgtcggc cggagccggg actactgccg ccgctcctgc cgccagcggg
     1201 cgtacaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1261 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1321 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1381 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atagctttaa
     1441 tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg caccgtgtat gaaatctaac
     1501 aatgcgctca tcgtcatcct cggcaccgtc accctggatg ctgtaggcat aggcttggtt
     1561 atgccggtac tgccgggcct cttgcgggat aactcgacat cttggttacc gtgaagttac
     1621 catcacggaa aaaggttatg ctgcttttaa gacccacttt cacatttaag ttgtttttct
     1681 aatccgcata tgatcaattc aaggccgaat aagaaggctg gctctgcacc ttggtgatca
     1741 aataattcga tagcttgtcg taataatggc ggcatactat cagtagtagg tgtttccctt
     1801 tcttctttag cgacttgatg ctcttgatct tccaatacgc aacctaaagt aaaatgcccc
     1861 acagcgctga gtgcatataa tgcattctct agtgaaaaac cttgttggca taaaaaggct
     1921 aattgatttt cgagagtttc atactgtttt tctgtaggcc gtgtacctaa atgtactttt
     1981 gctccatcgc gatgacttag taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa
     2041 tcttgccagc tttccccttc taaagggcaa aagtgagtat ggtgcctatc taacatctca
     2101 atggctaagg cgtcgagcaa agcccgctta ttttttacat gccaatacaa tgtaggctgc
     2161 tctacaccta gcttctgggc gagtttacgg gttgttaaac cttcgattcc gacctcatta
     2221 agcagctcta atgcgctgtt aatcacttta cttttatcta atctagacat catgttgtgt
     2281 ctcctctaaa gattgtagga aatgcaatgt gtcaagcagt taggtcaatc agagcatcgg
     2341 tcactcaaga attcttgagc tgacagaacc caagggcgag tatggcgaat ttttccaact
     2401 cacgcaccaa acctactcac attggataat tcgtcgagtc aactccgtgt caacctgtcc
     2461 tcaactttag cggggaaaca aagatttcgc agcagatgca aaatcataca gacacattca
     2521 aaaatggaca ttgaccccaa acgggggcat ttccaacact tgcgctaaat agtttttgca
     2581 gggaacgaac ctgaaatcgt tcggatcgta cgcgtttgtt gaactaatgg gtgctttagt
     2641 tgaagaataa aagaccacat taaaaaatgt ggtcttttgt gtttttttaa aggatttgag
     2701 cgtagcgaaa aatccttttc tttcttatct tgataataag ggtaactatt gccgatgata
     2761 agctgtcaaa catgagaatt ccaaaaacta aaaaaaatat tgacactcta tcattgatag
     2821 agtataatta acgggatcct ctatcaatga tagaggatcc cgccaagctt aagatcccca
     2881 gcttgttgat acacctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca
     2941 ctggccgtcg ttttacagcc aagcttggct gttttggcgg atgagagaag attttcagcc
     3001 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
     3061 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
     3121 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
     3181 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     3241 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     3301 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
     3361 acggatggcc tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa
     3421 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3481 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3541 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3601 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3661 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3721 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3781 cttggttgag taattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     3841 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3901 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3961 gtcgtgccag tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     4021 ccattatcgc cggcatggcg gccgacgcgc ggggagaggc ggtttgcgta ttgggcgcca
     4081 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc
     4141 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt
     4201 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta
     4261 ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg
     4321 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca
     4381 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa
     4441 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac
     4501 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc
     4561 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga
     4621 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt
     4681 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg
     4741 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca
     4801 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac
     4861 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt
     4921 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa
     4981 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg
     5041 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc
     5101 gctatcatgc cataccgcga aaggttttgc accattcgat ggtgtcaacg taaatgcatg
     5161 ccgcttcgcc ttcgcgcgcg aattgcaagc tgatccgggc ttatcgactg cacggtgcac
     5221 caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat
     5281 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg
     5341 acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc
     5401 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacaga attaaaagat
     5461 atgaccatga ttacgccaag cttgcggcgg ccgcaccagg tatttaaatc catggcgggt
     5521 accgctagcg aattcactgg ccgtcgtttt acagccaagc ttggctgttt tggcggatga
     5581 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
     5641 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
     5701 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
     5761 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     5821 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
     5881 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
     5941 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgttataact
     6001 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6061 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6121 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6181 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6661 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6901 attatcaaaa aggatcttca cctagatcct tttggggggg gggggaaagc cacgttgtgt
     6961 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac
     7021 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
     7081 cttgctcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     7141 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg
     7201 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     7261 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     7321 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg
     7381 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     7441 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     7501 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     7561 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataagctt ttgccattct
     7621 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     7681 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     7741 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     7801 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     7861 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     7921 gacgggacgg cggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac
     7981 gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg
     8041 gtccacctac aacaaagctc tcatcaaccg tggctccctc actttctggc tggatgatgg
     8101 ggcgattcag gcctggtatg agtcagcaac accttcttca cgaggcagac ctcagcgccc
     8161 ccccccccct agcttgtcta cgtctgatgc tttgaatcgg acggacttgc cgatcttgta
     8221 tgcggtgatt tttccctcgt ttgcccactt tttaatggtg gccggggtga gagctacgcg
     8281 ggcggcgacc tgctgcgctg tgatccaata ttcggggtcg ttcactggtt cccctttctg
     8341 atttctggca tagaagaacc cccgtgaact gtgtggttcc gggggttgct gatttttgcg
     8401 agacttctcg cgcaattccc tagcttaggt gaaaacacca tgaaacacta gggaaacacc
     8461 catgaaacac ccattagggc agtagggcgg cttcttcgtc tagggcttgc atttgggcgg
     8521 tgatctggtc tttagcgtgt gaaagtgtgt cgtaggtggc gtgctcaatg cactcgaacg
     8581 tcacgtcatt taccgggtca cggtgggcaa agagaactag tgggttagac attgttttcc
     8641 tcgttgtcgg tggtggtgag cttttctagc cgctcggtaa acgcggcgat catgaactct
     8701 tggaggtttt caccgttctg catgcctgcg cgcttcatgt cctcacgtag tgccaaagga
     8761 acgcgtgcgg tgaccacgac gggcttagcc tttgcctgcg cttctagtgc ttcgatggtg
     8821 gcttgtgcct gcgcttgctg cgcctgtagt gcctgttgag cttcttgtag ttgctgttct
     8881 agctgtgcct tggttgccat gctttaagac tctagtagct ttcctgcgat atgtcatgcg
     8941 catgcgtagc aaacattgtc ctgcaactca ttcattatgt gcagtgctcc tgttactagt
     9001 cgtacatact catatttacc tagtctgcat gcagtgcatg cacatgcagt catgtcgtgc
     9061 taatgtgtaa aacatgtaca tgcagattgc tgggggtgca gggggcggag ccaccctgtc
     9121 catgcggggt gtggggcttg ccccgccggt acagacagtg agcaccgggg cacctagtcg
     9181 cggatacccc ccctaggtat cggacacgta accctcccat gtcgatgcaa atctttaaca
     9241 ttgagtacgg gtaagctggc acgcatagcc aagctaggcg gccaccaaac accactaaaa
     9301 attaatagtt cctagacaag acaaaccccc gtgcgagcta ccaactcata tgcacggggg
     9361 ccacataacc cgaaggggtt tcaattgaca accatagcac tagctaagac aacgggcaca
     9421 acacccgcac aaactcgcac tgcgcaaccc cgcacaacat cgggtctagg taacactgaa
     9481 atagaagtga acacctctaa ggaaccgcag gtcaatgagg gttctaaggt cactcgcgct
     9541 agggcgtggc gtaggcaaaa cgtcatgtac aagatcacca atagtaaggc tctggcgggg
     9601 tgccataggt ggcgcaggga cgaagctgtt gcggtgtcct ggtcgtctaa cggtgcttcg
     9661 cagtttgagg gtctgcaaaa ctctcactct cgctgggggt cacctctggc tgaattggaa
     9721 gtcatgggcg aacgccgcat tgagctggct attgctacta agaatcactt ggcggcgggt
     9781 ggcgcgctca tgatgtttgt gggcactgtt cgacacaacc gctcacagtc atttgcgcag
     9841 gttgaagcgg gtattaagac tgcgtactct tcgatggtga aaacatctca gtggaagaaa
     9901 gaacgtgcac ggtacggggt ggagcacacc tatagtgact atgaggtcac agactcttgg
     9961 gcgaacg
//
