Basic Vector Information
- Vector Name:
- pNZ11
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4925 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Zhang N, Magee BB, Magee PT, Cannon R, Schmid J.
pNZ11 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNZ11 vector Sequence
LOCUS 40924_33692 4925 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNZ11, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4925)
AUTHORS Zhang N, Magee BB, Magee PT, Cannon R, Schmid J.
TITLE A method for mating clinical Candida albicans isolates
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4925)
AUTHORS Zhang N, Magee BB, Magee PT, Cannon R, Schmid J.
TITLE Direct Submission
JOURNAL Submitted (04-MAR-2009) IMBS, Massey University, Riddet Road,
Palmerston North 4410, New Zealand
REFERENCE 3 (bases 1 to 4925)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4925)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(04-MAR-2009) IMBS, Massey University, Riddet Road, Palmerston North
4410, New Zealand"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4925
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory complement(657..1046)
/label=Candida albicans tACT1
/note="Candida albicans tACT1"
/regulatory_class="terminator"
CDS complement(1066..1629)
/label=NrsR
/note="nourseothricin acetyltransferase"
primer_bind complement(1685..1701)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
regulatory complement(1704..2723)
/label=Candida albicans PACT1
/note="Candida albicans PACT1"
/regulatory_class="promoter"
promoter complement(2737..2755)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(2776..2792)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(2800..2816)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2824..2854)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(2869..2890)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3178..3766)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3940..4797)
/label=AmpR
/note="beta-lactamase"
promoter complement(4798..4902)
/label=AmpR promoter
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