LOCUS       40924_33692        4925 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNZ11, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Zhang N, Magee BB, Magee PT, Cannon R, Schmid J.
  TITLE     A method for mating clinical Candida albicans isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Zhang N, Magee BB, Magee PT, Cannon R, Schmid J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2009) IMBS, Massey University, Riddet Road, 
            Palmerston North 4410, New Zealand
REFERENCE   3  (bases 1 to 4925)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4925)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-MAR-2009) IMBS, Massey University, Riddet Road, Palmerston North
            4410, New Zealand"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      complement(657..1046)
                     /label=Candida albicans tACT1
                     /note="Candida albicans tACT1"
                     /regulatory_class="terminator"
     CDS             complement(1066..1629)
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     primer_bind     complement(1685..1701)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(1704..2723)
                     /label=Candida albicans PACT1
                     /note="Candida albicans PACT1"
                     /regulatory_class="promoter"
     promoter        complement(2737..2755)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2776..2792)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2800..2816)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2824..2854)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2869..2890)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3178..3766)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3940..4797)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4798..4902)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcat
      661 tttatgatgg aatgaatggg atgaatcatc aaacaagaga aaatacccgt gacgaaaata
      721 ataaaataag ttcctctgat acagaagatg aaaacaacaa caacaagata tagaaatgcc
      781 ttgggtggct attttatagt cttaactttt taatgtatat ttgttttgtt tttttacata
      841 ataatacttt ataaaagcta agctaaattc aagtaaaatt tcaatctctc aaataaaaca
      901 tttttctctt tttcttaaat ttagttttat atatttataa aataaacaaa gattttttta
      961 aaaaagtaac aagttatata tgtaataaca aaaagaagaa taacaagaat acaaaaccag
     1021 atttccagat ttccagaatt tcactcggat ccccccgagt tattatggac atggcataga
     1081 catatacaaa gcttgttcac catcggaagc agtaccatcg tataaagcag tatccaaacc
     1141 acacaaagtg aaacccattc ttctataagc atgaatagct ggagcattaa cattggtaac
     1201 ttccaaccac aaatgaccag cacctctttc tctggcgaat tcagtagcca aacccatcaa
     1261 agctctacca acaccatgac ctctatgttc tggagcaact tcaatatctt caacagtcaa
     1321 tcttctgttc caaccagaat aagaaacaac aacgaaacca gccaaatcac catcatcacc
     1381 ataagcaacg aaagttctag aatctggatc accatcttca ccagcatcgg attcatcatc
     1441 ggattcatca tctgggaaaa ccttagtcaa tggtggatca actggaactt ctctcaaagt
     1501 gaaaccatca ccagtagcag taactctaaa aacagtatcg gtagtgaaag aaccatccaa
     1561 agcttcaata gcttcagcat cacctggaac agaagttctg tatctataag cagtatcatc
     1621 caaagtagta gacataattg taggatcctt cctgaattct gcaggaattc gatatcaagc
     1681 ttatcgatac cgtcgacctc gagtttgaat gattatattt ttttaatatt aatatcgaga
     1741 taatgtttct aagaaagaaa gaaaaccagg agtgaaaatt agaaaaggaa aggaaaggaa
     1801 aaaaagaaaa atctgaaaat atataaaaaa aaattgtttc gttggcaata aatcttggtg
     1861 agaacagcga ccgaaagcaa ataagaacaa aatatgagtg tattacgttg aacaactaat
     1921 taacgtgtgt gtatggatct tttttctttt ttctctttaa ccgactataa acaacaaaca
     1981 tttttgggca gtgcacacac tacttaatat acacagcata aattacacga ttagaaacaa
     2041 attagcttat taaaataacc taatcaaacc gaatatttta tggtattatg agtaaactat
     2101 ataatataaa tagcacacac ccacaacaac aacaaaggaa aactaaaagg ttttttcttt
     2161 ttgaaaagat cgttttcttt attattctct agttttgacg ctgaaatatt cgttgtactg
     2221 ttgcaaattt gtatttggtc tgccttatct tcttttctac actttttact tatgagcttt
     2281 ataattcaca gataagtttc aggtttgcca gatatacttg cttcactggt tactaaactc
     2341 tcggtgtaca aatgacttgc ttatgtaaga ggctactttt ctagttttgc agtcattcat
     2401 ttaaaaacaa ttgatcctga aggctctaaa gacatagtag gtttactttg actgtatatt
     2461 tccgtggcat gtttaaaggc cccttggcca taggatattg gtggcggcac agtaagcatt
     2521 aaaattgttg tttcgcggaa ttacaacatt tcttctttta cataataatg tcactccttt
     2581 ttgattggca tagccgagat ctttttttaa cacagacttt aagctgtttt ttgccacctg
     2641 agaacagttt ctattttggt ccaaaagaaa tattagacta agtttattat aaacgaggct
     2701 ggtgatctta atagctctgg tacccagctt ttgttccctt tagtgagggt taattgcgcg
     2761 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     2821 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     2881 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     2941 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3001 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3061 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3121 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3181 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3241 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3301 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3361 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3421 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3481 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3541 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3601 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3661 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3721 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3781 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3841 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3901 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3961 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4021 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4081 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4141 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4201 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4261 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4321 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4381 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4441 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4501 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4561 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4621 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4681 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4741 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4801 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4861 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4921 gccac
//
