Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V004246 | pNW33N | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
AmpR fragment is non-functional - Chloramphenicol should be used as the resistance marker.
- Vector Name:
- pNW33N
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 4217 bp
- Type:
- Geobacillus stearothermophilus Expression
- Replication origin:
- ori
- Source/Author:
- Mee EK, Welker NE.
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pNW33N vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Nilasari D, Dover N, Rech S, Komives C. Expression of recombinant green fluorescent protein in Bacillus methanolicus. Biotechnol Prog. 2012 May-Jun;28(3):662-8.
pNW33N vector Sequence
LOCUS 40924_33672 4217 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNW33N, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4217)
AUTHORS Mee EK, Welker NE.
TITLE Development of Genetic Tools to Analyze and Characterize Genes of
the Thermophile Geobacillus stearothermophilus
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4217)
AUTHORS Mee EK, Welker NE.
TITLE Direct Submission
JOURNAL Submitted (14-FEB-2003) Biochemistry, Molecular Biology, and
Cellular Biology, Northwestern University, 2205 Tech Drive,
Evanston, IL 60208, USA
REFERENCE 3 (bases 1 to 4217)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4217)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(14-FEB-2003) Biochemistry, Molecular Biology, and Cellular Biology,
Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4217
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..51
/label=multiple cloning site from plasmid pUC19
/note="multiple cloning site from plasmid pUC19"
primer_bind complement(69..85)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(93..109)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(117..147)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(162..183)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(316..963)
/gene="cat"
/label=cat
/note="Chloramphenicol acetyltransferase from
Staphylococcus aureus. Accession#: P00485"
CDS complement(1073..2074)
/label=repB
/note="RepB replication protein"
rep_origin complement(2766..3354)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 4217
/label=MCS
/note="pUC18/19 multiple cloning site"