LOCUS       40924_33672        4217 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNW33N, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Mee EK, Welker NE.
  TITLE     Development of Genetic Tools to Analyze and Characterize Genes of 
            the Thermophile Geobacillus stearothermophilus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Mee EK, Welker NE.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2003) Biochemistry, Molecular Biology, and 
            Cellular Biology, Northwestern University, 2205 Tech Drive, 
            Evanston, IL 60208, USA
REFERENCE   3  (bases 1 to 4217)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4217)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-FEB-2003) Biochemistry, Molecular Biology, and Cellular Biology,
            Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..51
                     /label=multiple cloning site from plasmid pUC19
                     /note="multiple cloning site from plasmid pUC19"
     primer_bind     complement(69..85)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(93..109)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(117..147)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(162..183)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(316..963)
                     /gene="cat"
                     /label=cat
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(1073..2074)
                     /label=repB
                     /note="RepB replication protein"
     rep_origin      complement(2766..3354)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4217
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
ORIGIN
        1 aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggcg
       61 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      121 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      181 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcccttc
      241 aaacttccca aaggcgagcc ctagtgacat tagaaaaccg actgtaaaaa gtacagtcgg
      301 cattatctca tattataaaa gccagtcatt aggcctatct gacaattcct gaatagagtt
      361 cataaacaat cctgcatgat aaccatcaca aacagaatga tgtacctgta aagatagcgg
      421 taaatatatt gaattacctt tattaatgaa ttttcctgct gtaataatgg gtagaaggta
      481 attactatta ttattgatat ttaagttaaa cccagtaaat gaagtccatg gaataataga
      541 aagagaaaaa gcattttcag gtataggtgt tttgggaaac aatttccccg aaccattata
      601 tttctctaca tcagaaaggt ataaatcata aaactctttg aagtcattct ttacaggagt
      661 ccaaatacca gagaatgttt tagatacacc atcaaaaatt gtataaagtg gctctaactt
      721 atcccaataa cctaactctc cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt
      781 tatcaccctt gtcactaaga aaataaatgc agggtaaaat ttatatcctt cttgttttat
      841 gtttcggtat aaaacactaa tatcaatttc tgtggttata ctaaaagtcg tttgttggtt
      901 caaataatga ttaaatatct cttttctctt ccaattgtct aaatcaattt tattaaagtt
      961 catttgatat gcctcctaaa tttttatcta aagtgaattt aggaggctta cttgtctgct
     1021 ttcttcatta gaatcaatcc ttttttaaaa gtcaatcccg tttgttgaac tactctttaa
     1081 taaaataatt tttccgttcc caattccaca ttgcaataat agaaaatcca tcttcatcgg
     1141 ctttttcgtc atcatctgta tgaatcaaat cgccttcttc tgtgtcatca aggtttaatt
     1201 ttttatgtat ttcttttaac aaaccaccat aggagattaa ccttttacgg tgtaaacctt
     1261 cctccaaatc agacaaacgt ttcaaattct tttcttcatc atcggtcata aaatccgtat
     1321 cctttacagg atattttgca gtttcgtcaa ttgccgattg tatatccgat ttatatttat
     1381 ttttcggtcg aatcatttga acttttacat ttggatcata gtctaatttc attgcctttt
     1441 tccaaaattg aatccattgt ttttgattca cgtagttttc tgtattctta aaataagttg
     1501 gttccacaca taccaataca tgcatgtgct gattataaga attatcttta ttatttattg
     1561 tcacttccgt tgcacgcata aaaccaacaa gatttttatt aattttttta tattgcatca
     1621 ttcggcgaaa tccttgagcc atatctgaca aactcttatt taattcttcg ccatcataaa
     1681 catttttaac tgttaatgtg agaaacaacc aacgaactgt tggcttttgt ttaataactt
     1741 cagcaacaac cttttgtgac tgaatgccat gtttcattgc tctcctccag ttgcacattg
     1801 gacaaagcct ggatttacaa aaccacactc gatacaactt tctttcgcct gtttcacgat
     1861 tttgtttata ctctaatatt tcagcacaat cttttactct ttcagccttt ttaaattcaa
     1921 gaatatgcag aagttcaaag taatcaacat tagcgatttt cttttctctc catggtctca
     1981 cttttccact ttttgtcttg tccactaaaa cccttgattt ttcatctgaa taaatgctac
     2041 tattaggaca cataatatta aaagaaaccc ccatctattt agttatttgt ttggtcactt
     2101 ataactttaa cagatggggt ttttctgtgc aaccaatttt aagggttttc aatactttaa
     2161 aacacataca taccaacact tcaacgcacc tttcagcaac taaaataaaa atgacgttat
     2221 ttctatatgt atcaagaata gaaagaactc gtttttcgct acgctcaaaa cgcaaaaaaa
     2281 gcactcattc gagtgctttt tcttatcgct ccaaatcatg cgattttttc ctctttgctt
     2341 ttctttgctc acgaagttct cgatcacgct gcaaaacatc ttgaagcgaa aaagtattct
     2401 tcttttcttc cgatcgctca tgctgacgca cgaaaagccc tctaggcgca taggaacaac
     2461 tcctaaatgc atgtgagggg ttttctcgtc catgtgaaca gtcgcatacg caatattttg
     2521 tttcccatac tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2581 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2641 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2701 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2761 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2821 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2881 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2941 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3001 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3061 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3121 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3181 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3241 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3301 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3361 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3421 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3481 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3541 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3601 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3661 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agcccgatat
     3721 gggaaacaaa atattgcgta tgcgactgtt cacatggacg agaaaacccc tcacatgcat
     3781 ttaggagttg ttcctatgcg cctagagggc ttttcgtgcg tcagcatgag cgatcggaag
     3841 aaaagaagaa tactttttcg cttcaagatg ttttgcagcg tgatcgagaa cttcgtgagc
     3901 aaagaaaagc aaagaggaaa aaatcgcatg atttggagcg ataagaaaaa gcactcgaat
     3961 gagtgctttt tttgcgtttt gagcgtagcg aaaaacgagt tctttctatt cttgatacat
     4021 atagaaataa cgtcattttt attttagttg ctgaaaggtg cgttgaagtg ttggtatgta
     4081 tgtgattcaa taatttcttt tactcgctcg ttatagtcga tcggttcatc attcaccaaa
     4141 tcataatttt catgtgaccg ttctttatca atatcgggat tcgttttact ttcccgttct
     4201 ctctgattgt gaaattg
//
