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pNV18Sm vector (V004249)

Basic Vector Information

Vector Name:
pNV18Sm
Antibiotic Resistance:
Streptomycin
Length:
5567 bp
Type:
Dietzia-Escherichia shuttle vector
Replication origin:
ori
Source/Author:
Szvetnik A, Bihari Z, Szabo Z, Kelemen O, Kiss I.

pNV18Sm vector Vector Map

pNV18Sm5567 bp60012001800240030003600420048005400SmRoriCAP binding sitelac promoterlac operatorM13 revMCSM13 fwdRepA

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNV18Sm vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_33657        5567 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Dietzia-Escherichia shuttle vector pNV18Sm, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Szvetnik A, Bihari Z, Szabo Z, Kelemen O, Kiss I.
  TITLE     Genetic manipulation tools for Dietzia spp
  JOURNAL   J. Appl. Microbiol. 109 (5), 1845-1852 (2010)
  PUBMED    20666867
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Bihari Z, Szvetnik A.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2009) Dep. of Appl. Microbiol., BAYBIO, Derkovits 
            fasor 2., Szeged, Csongrad H-6726, Hungary
REFERENCE   3  (bases 1 to 5567)
  AUTHORS   Bihari Z, Szvetnik A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2011) Dep. of Appl. Microbiol., BAYBIO, Derkovits 
            fasor 2., Szeged, Csongrad H-6726, Hungary
REFERENCE   4  (bases 1 to 5567)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5567)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Appl. 
            Microbiol."; date: "2010"; volume: "109"; issue: "5"; pages: 
            "1845-1852"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2009) Dep. of Appl. Microbiol., BAYBIO, Derkovits fasor 2., 
            Szeged, Csongrad H-6726, Hungary"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (23-FEB-2011) Dep. of Appl. Microbiol., BAYBIO, Derkovits fasor 2., 
            Szeged, Csongrad H-6726, Hungary"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On Feb 23, 2011 this sequence version replaced GQ495223.1.
FEATURES             Location/Qualifiers
     source          1..5567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             859..1647
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2273..2861
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3149..3170
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3185..3215
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3223..3239
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3247..3263
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3273..3329
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3333..3349)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3896..4822
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="replication protein"
                     /protein_id="ACV66989.1"
                     /translation="MSHVADEFEQLWLPYWPLASDDLLEGIYRQSRASALGRRYIEANP
                     TALANLLVVDVDHPDAALRALSARGSHPLPNAIVGNRANGHAHAVWALNAPVPRTEYAR
                     RKPLAYMAACAEGLRRAVDGDRSYSGLMTKNPGHIAWETEWLHSDLYTLSHIEAELGAN
                     MPPPRWRQQTTYKAAPTPLGRNCALFDSVRLWAYRPALMRIYLPTRNVDGLGRAIYAEC
                     HARNAEFPCNDVCPGPLPDSEVRAIANSIWRWITTKSRIWADGIVVYEATLSARQSAIS
                     RKGAAARTAASTVARRAKSASAMEALL"
     gene            3896..4822
                     /gene="repA"
                     /label=repA
     CDS             4819..5154
                     /codon_start=1
                     /gene="repB"
                     /product="RepB"
                     /label=repB
                     /note="replication protein"
                     /protein_id="ACV66990.2"
                     /translation="MSDGYNRQPTVRKKRRVTAAEGARITGLSERHVVRLVAQERSEWL
                     AEQAARRERIRAYHDDEGHSWPQTAKHFGLHLDTVKRLGYRARKERAAEQEAAQKAHNE
                     ADNPPLF"
     gene            4819..5154
                     /gene="repB"
                     /label=repB

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