LOCUS       40924_33657        5567 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Dietzia-Escherichia shuttle vector pNV18Sm, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Szvetnik A, Bihari Z, Szabo Z, Kelemen O, Kiss I.
  TITLE     Genetic manipulation tools for Dietzia spp
  JOURNAL   J. Appl. Microbiol. 109 (5), 1845-1852 (2010)
  PUBMED    20666867
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Bihari Z, Szvetnik A.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2009) Dep. of Appl. Microbiol., BAYBIO, Derkovits 
            fasor 2., Szeged, Csongrad H-6726, Hungary
REFERENCE   3  (bases 1 to 5567)
  AUTHORS   Bihari Z, Szvetnik A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2011) Dep. of Appl. Microbiol., BAYBIO, Derkovits 
            fasor 2., Szeged, Csongrad H-6726, Hungary
REFERENCE   4  (bases 1 to 5567)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5567)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Appl. 
            Microbiol."; date: "2010"; volume: "109"; issue: "5"; pages: 
            "1845-1852"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2009) Dep. of Appl. Microbiol., BAYBIO, Derkovits fasor 2., 
            Szeged, Csongrad H-6726, Hungary"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (23-FEB-2011) Dep. of Appl. Microbiol., BAYBIO, Derkovits fasor 2., 
            Szeged, Csongrad H-6726, Hungary"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On Feb 23, 2011 this sequence version replaced GQ495223.1.
FEATURES             Location/Qualifiers
     source          1..5567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             859..1647
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2273..2861
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3149..3170
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3185..3215
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3223..3239
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3247..3263
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3273..3329
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3333..3349)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3896..4822
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="replication protein"
                     /protein_id="ACV66989.1"
                     /translation="MSHVADEFEQLWLPYWPLASDDLLEGIYRQSRASALGRRYIEANP
                     TALANLLVVDVDHPDAALRALSARGSHPLPNAIVGNRANGHAHAVWALNAPVPRTEYAR
                     RKPLAYMAACAEGLRRAVDGDRSYSGLMTKNPGHIAWETEWLHSDLYTLSHIEAELGAN
                     MPPPRWRQQTTYKAAPTPLGRNCALFDSVRLWAYRPALMRIYLPTRNVDGLGRAIYAEC
                     HARNAEFPCNDVCPGPLPDSEVRAIANSIWRWITTKSRIWADGIVVYEATLSARQSAIS
                     RKGAAARTAASTVARRAKSASAMEALL"
     gene            3896..4822
                     /gene="repA"
                     /label=repA
     CDS             4819..5154
                     /codon_start=1
                     /gene="repB"
                     /product="RepB"
                     /label=repB
                     /note="replication protein"
                     /protein_id="ACV66990.2"
                     /translation="MSDGYNRQPTVRKKRRVTAAEGARITGLSERHVVRLVAQERSEWL
                     AEQAARRERIRAYHDDEGHSWPQTAKHFGLHLDTVKRLGYRARKERAAEQEAAQKAHNE
                     ADNPPLF"
     gene            4819..5154
                     /gene="repB"
                     /label=repB
ORIGIN
        1 gggtaccgag ctcgatgctt gtaaaccgtt ttgtgaaaaa atttttaaaa taaaaaaggg
       61 gacctctagg gtccccaatt aattagtaat ataatctatt aaaggtcatt caaaaggtca
      121 tccaccggat caattcccct gctcgcgcag gctgggtgcc aagctctcgg gtaacatcaa
      181 ggcccgatcc ttggagccct tgccctcccg cacgatgatc gtgccgtgat cgaaatccag
      241 atccttgacc cgcagttgca aaccctcact gatccgcatg cccgttccat acagaagctg
      301 ggcgaacaaa cgatgctcgc cttccagaaa accgaggatg cgaaccactt catccggggt
      361 cagcaccacc ggcaagcgcc gcgacggccg aggtcttccg atctcctgaa gccagggcag
      421 atccgtgcac agaccttgcc gtagaagaac agcaaggccg ccaatgcctg acgatgcgtg
      481 gagaccgaaa ccttgcgctc gttcgccagc caggacagaa atgcctcgac ttcgctgctg
      541 cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca
      601 taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc
      661 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
      721 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
      781 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
      841 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
      901 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
      961 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     1021 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     1081 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     1141 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     1201 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     1261 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     1321 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     1381 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     1441 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     1501 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     1561 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     1621 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc
     1681 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     1741 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     1801 tgggagatat atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat
     1861 cgcaacatcc gcattaaaat ctagcgaggg ctttactaag ctgatccggt ggatgacctt
     1921 ttgaatgacc tttaatagat tatattacta attaattggg gaccctagag gtcccctttt
     1981 ttattttaaa aattttttca caaaacggtt tacaagcatc gagctcggta cccctgccat
     2041 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     2101 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     2161 ccaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2221 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2281 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2341 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2401 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2461 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2521 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2581 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2641 aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg
     2701 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2761 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2821 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2881 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     2941 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3001 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     3061 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     3121 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     3181 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     3241 ttcacacagg aaacagctat gaccatgatt acgaattcga gctcggtacc cggggatcct
     3301 ctagagtcga cctgcaggca tgcaagcttg gcactggccg tcgttttaca acgtcgtgac
     3361 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     3421 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     3481 ggcgaatggc gcgataagct agcgtgagta gcgcgtacct cacctcgccc aacaggtcgc
     3541 acacagccga ttcgtacgcc ataaagccag gtgagcccac cagctccgta agttcgggcg
     3601 ctgtgtggct cgtacccgcg cattcaggcg gcagggggtc taacgggtct aaggcggcgt
     3661 gtacggccgc cacagcggct ctcagcggcc cggaaacgtc ctcgaaacga cgcatgtgtt
     3721 cctcctggtt ggtacaggtg gttgggggtg ctcggctgtc gctggtgttc caccaccagg
     3781 gctcgacggg agagcggggg agtgtgcagt tgtggggtgg cccctcagcg aaatatctga
     3841 cttggagctc gtgtcggacc atacaccggt gattaatcgt ggtctactac caagcgtgag
     3901 ccacgtcgcc gacgaatttg agcagctctg gctgccgtac tggccgctgg caagcgacga
     3961 tctgctcgag gggatctacc gccaaagccg cgcgtcggcc ctaggccgcc ggtacatcga
     4021 ggcgaaccca acagcgctgg caaacctgct ggtcgtggac gtagaccatc cagacgcagc
     4081 gctccgagcg ctcagcgccc gggggtccca tccgctgccc aacgcgatcg tgggcaatcg
     4141 cgccaacggc cacgcacacg cagtgtgggc actcaacgcc cctgttccac gcaccgaata
     4201 cgcgcggcgt aagccgctcg catacatggc ggcgtgcgcc gaaggccttc ggcgcgccgt
     4261 ggacggcgac cgcagttact caggcctcat gaccaaaaac cccggccaca tcgcctggga
     4321 aacggaatgg ctccactcag atctctacac actcagccac atcgaggccg agctcggcgc
     4381 gaacatgcca ccgccgcgct ggcgtcagca gaccacgtac aaagcggctc cgacgccgct
     4441 agggcggaat tgcgcactgt tcgattccgt caggttgtgg gcctatcgtc ccgccctcat
     4501 gcggatctac ctgccgaccc ggaacgtgga cggactcggc cgcgcgatct atgccgagtg
     4561 ccacgcgcga aacgccgagt tcccgtgcaa cgacgtgtgt cccggaccgc taccggacag
     4621 cgaggtccgc gccatcgcca acagcatttg gcgttggatc acaaccaagt cgcgcatttg
     4681 ggcggacggg atcgtggtct acgaggccac actcagtgcg cgccagtcgg ccatctcgcg
     4741 gaagggcgca gcagcgcgca cggcggcgag cacagttgcg cggcgcgcaa agtccgcgtc
     4801 agccatggag gcattgctat gagcgacggc tacaaccggc agccgactgt ccgcaaaaag
     4861 cggcgcgtga ccgccgccga aggcgctcga atcaccggac tatccgaacg ccacgtcgtc
     4921 cggctcgtgg cgcaggaacg cagcgagtgg ctcgccgagc aggctgcacg ccgcgaacgc
     4981 atccgcgcct atcacgacga cgagggccac tcttggccgc aaacggccaa acatttcggg
     5041 ctgcatctgg acaccgttaa gcgactcggc tatcgggcga ggaaagagcg tgcggcagaa
     5101 caggaagcgg ctcaaaaggc ccacaacgaa gccgacaatc caccgctgtt ctaacgcaat
     5161 tggggagcgg gtgtcgcggg ggttccgtgg ggggttccgt tgcaacgggt cggacaggta
     5221 aaagtcctgg tagacgctag cttcacgctg ccgcaagcac tcagggcgca agggctgcta
     5281 aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
     5341 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
     5401 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
     5461 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
     5521 tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatc
//
