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pNUversa vector (V004251)

Basic Vector Information

Vector Name:
pNUversa
Antibiotic Resistance:
Ampicillin
Length:
10873 bp
Type:
Cloning vector
Replication origin:
ori
Source/Author:
Sakai F, Sonaty G, Watson D, Klugman KP, Vidal JE.
Promoter:
tac

pNUversa vector Vector Map

pNUversa10873 bp5001000150020002500300035004000450050005500600065007000750080008500900095001000010500M13 fwdtac promoterlac operatorreal time PCR target sequence - Streptococcus pneumoniae lytA gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 1 cps gene fragmentreal time PCR target sequence -Streptococcus pneumoniae serotype 2 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 3 cps gene fragmentreal time PCR target sequence -Streptococcus pneumoniae serotype 4 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 5 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 6ABCD cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 6CD cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 7AF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 7BC/40 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 7C cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 8 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 9VA cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 9LN cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 10A cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 10B cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 10CF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 11AD cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 11BC cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 11F cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 12ABF/44/46 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 13 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 14 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 15AF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 15ABCF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 16A cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 16F cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 17A cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 17F cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 18ABCF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 19A cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 19BF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 19F cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 19'F' cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 19C cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 20 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 21 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 22AF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 23A cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 23B cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 23F cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 24A cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 24BF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 25AF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 27 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 28AF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 29 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 31 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 32AF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 33AF/37 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 33B cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 33C cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 33D cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 34 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 35AC/42 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 35B cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 35F/47F cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 36 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 38 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 39 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 41A cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 41F cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 43 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 45 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 46 cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 47AF cps gene fragmentreal time PCR target sequence - Streptococcus pneumoniae serotype 48 cps gene fragmentM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNUversa vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_33647       10873 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNUversa, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10873)
  AUTHORS   Sakai F, Sonaty G, Watson D, Klugman KP, Vidal JE.
  TITLE     Development and characterization of a synthetic DNA, NUversa, to be 
            used as a standard in quantitative polymerase chain reactions for 
            molecular pneumococcal serotyping
  JOURNAL   FEMS Microbiol. Lett. 364 (17) (2017)
  PUBMED    28903467
REFERENCE   2  (bases 1 to 10873)
  AUTHORS   Sakai F, Vidal JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2017) Hubert Department of Global Health, Rollins 
            School of Public Health, Emory University, 1518 Clifton Rd., 
            Atlanta, GA 30329, USA
REFERENCE   3  (bases 1 to 10873)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10873)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett. 364 (17) (2017)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUL-2017) Hubert Department of Global Health, Rollins School of 
            Public Health, Emory University, 1518 Clifton Rd., Atlanta, GA 
            30329, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        396..424
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    432..448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    461..535
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae lytA gene fragment"
     misc_feature    536..696
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 1 cps gene fragment"
     misc_feature    697..807
                     /note="real time PCR target sequence -Streptococcus
                     pneumoniae serotype 2 cps gene fragment"
     misc_feature    808..892
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 3 cps gene fragment"
     misc_feature    893..1106
                     /note="real time PCR target sequence -Streptococcus
                     pneumoniae serotype 4 cps gene fragment"
     misc_feature    1107..1188
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 5 cps gene fragment"
     misc_feature    1189..1316
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 6ABCD cps gene fragment"
     misc_feature    1317..1465
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 6CD cps gene fragment"
     misc_feature    1466..1569
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 7AF cps gene fragment"
     misc_feature    1570..1716
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 7BC/40 cps gene fragment"
     misc_feature    1717..1848
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 7C cps gene fragment"
     misc_feature    1849..1931
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 8 cps gene fragment"
     misc_feature    1932..2085
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 9VA cps gene fragment"
     misc_feature    2086..2200
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 9LN cps gene fragment"
     misc_feature    2201..2337
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 10A cps gene fragment"
     misc_feature    2338..2453
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 10B cps gene fragment"
     misc_feature    2454..2590
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 10CF cps gene fragment"
     misc_feature    2591..2697
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 11AD cps gene fragment"
     misc_feature    2698..2826
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 11BC cps gene fragment"
     misc_feature    2827..2919
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 11F cps gene fragment"
     misc_feature    2920..2999
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 12ABF/44/46 cps gene fragment"
     misc_feature    3000..3135
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 13 cps gene fragment"
     misc_feature    3136..3223
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 14 cps gene fragment"
     misc_feature    3224..3353
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 15AF cps gene fragment"
     misc_feature    3354..3446
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 15ABCF cps gene fragment"
     misc_feature    3447..3578
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 16A cps gene fragment"
     misc_feature    3579..3777
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 16F cps gene fragment"
     misc_feature    3778..3889
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 17A cps gene fragment"
     misc_feature    3890..4019
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 17F cps gene fragment"
     misc_feature    4020..4167
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 18ABCF cps gene fragment"
     misc_feature    4168..4256
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 19A cps gene fragment"
     misc_feature    4257..4331
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 19BF cps gene fragment"
     misc_feature    4332..4552
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 19F cps gene fragment"
     misc_feature    4553..4658
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 19'F' cps gene fragment"
     misc_feature    4659..4773
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 19C cps gene fragment"
     misc_feature    4774..4865
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 20 cps gene fragment"
     misc_feature    4866..4952
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 21 cps gene fragment"
     misc_feature    4953..5041
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 22AF cps gene fragment"
     misc_feature    5042..5127
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 23A cps gene fragment"
     misc_feature    5128..5205
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 23B cps gene fragment"
     misc_feature    5206..5431
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 23F cps gene fragment"
     misc_feature    5432..5551
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 24A cps gene fragment"
     misc_feature    5552..5684
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 24BF cps gene fragment"
     misc_feature    5685..5812
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 25AF cps gene fragment"
     misc_feature    5813..5891
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 27 cps gene fragment"
     misc_feature    5892..6032
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 28AF cps gene fragment"
     misc_feature    6033..6113
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 29 cps gene fragment"
     misc_feature    6114..6259
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 31 cps gene fragment"
     misc_feature    6260..6393
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 32AF cps gene fragment"
     misc_feature    6394..6498
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 33AF/37 cps gene fragment"
     misc_feature    6499..6644
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 33B cps gene fragment"
     misc_feature    6645..6791
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 33C cps gene fragment"
     misc_feature    6792..6872
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 33D cps gene fragment"
     misc_feature    6873..7016
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 34 cps gene fragment"
     misc_feature    7017..7124
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 35AC/42 cps gene fragment"
     misc_feature    7125..7248
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 35B cps gene fragment"
     misc_feature    7249..7393
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 35F/47F cps gene fragment"
     misc_feature    7394..7541
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 36 cps gene fragment"
     misc_feature    7542..7619
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 38 cps gene fragment"
     misc_feature    7620..7762
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 39 cps gene fragment"
     misc_feature    7763..7876
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 41A cps gene fragment"
     misc_feature    7877..8010
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 41F cps gene fragment"
     misc_feature    8011..8160
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 43 cps gene fragment"
     misc_feature    8161..8248
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 45 cps gene fragment"
     misc_feature    8249..8381
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 46 cps gene fragment"
     misc_feature    8382..8502
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 47AF cps gene fragment"
     misc_feature    8503..8635
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 48 cps gene fragment"
     primer_bind     complement(8652..8668)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8676..8692
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8700..8730)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8745..8766)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9054..9642)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9816..10673)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10674..10778)
                     /label=AmpR promoter

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