LOCUS       40924_33647       10873 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNUversa, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10873)
  AUTHORS   Sakai F, Sonaty G, Watson D, Klugman KP, Vidal JE.
  TITLE     Development and characterization of a synthetic DNA, NUversa, to be 
            used as a standard in quantitative polymerase chain reactions for 
            molecular pneumococcal serotyping
  JOURNAL   FEMS Microbiol. Lett. 364 (17) (2017)
  PUBMED    28903467
REFERENCE   2  (bases 1 to 10873)
  AUTHORS   Sakai F, Vidal JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2017) Hubert Department of Global Health, Rollins 
            School of Public Health, Emory University, 1518 Clifton Rd., 
            Atlanta, GA 30329, USA
REFERENCE   3  (bases 1 to 10873)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10873)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett. 364 (17) (2017)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUL-2017) Hubert Department of Global Health, Rollins School of 
            Public Health, Emory University, 1518 Clifton Rd., Atlanta, GA 
            30329, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        396..424
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    432..448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    461..535
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae lytA gene fragment"
     misc_feature    536..696
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 1 cps gene fragment"
     misc_feature    697..807
                     /note="real time PCR target sequence -Streptococcus
                     pneumoniae serotype 2 cps gene fragment"
     misc_feature    808..892
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 3 cps gene fragment"
     misc_feature    893..1106
                     /note="real time PCR target sequence -Streptococcus
                     pneumoniae serotype 4 cps gene fragment"
     misc_feature    1107..1188
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 5 cps gene fragment"
     misc_feature    1189..1316
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 6ABCD cps gene fragment"
     misc_feature    1317..1465
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 6CD cps gene fragment"
     misc_feature    1466..1569
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 7AF cps gene fragment"
     misc_feature    1570..1716
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 7BC/40 cps gene fragment"
     misc_feature    1717..1848
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 7C cps gene fragment"
     misc_feature    1849..1931
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 8 cps gene fragment"
     misc_feature    1932..2085
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 9VA cps gene fragment"
     misc_feature    2086..2200
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 9LN cps gene fragment"
     misc_feature    2201..2337
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 10A cps gene fragment"
     misc_feature    2338..2453
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 10B cps gene fragment"
     misc_feature    2454..2590
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 10CF cps gene fragment"
     misc_feature    2591..2697
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 11AD cps gene fragment"
     misc_feature    2698..2826
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 11BC cps gene fragment"
     misc_feature    2827..2919
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 11F cps gene fragment"
     misc_feature    2920..2999
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 12ABF/44/46 cps gene fragment"
     misc_feature    3000..3135
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 13 cps gene fragment"
     misc_feature    3136..3223
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 14 cps gene fragment"
     misc_feature    3224..3353
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 15AF cps gene fragment"
     misc_feature    3354..3446
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 15ABCF cps gene fragment"
     misc_feature    3447..3578
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 16A cps gene fragment"
     misc_feature    3579..3777
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 16F cps gene fragment"
     misc_feature    3778..3889
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 17A cps gene fragment"
     misc_feature    3890..4019
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 17F cps gene fragment"
     misc_feature    4020..4167
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 18ABCF cps gene fragment"
     misc_feature    4168..4256
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 19A cps gene fragment"
     misc_feature    4257..4331
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 19BF cps gene fragment"
     misc_feature    4332..4552
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 19F cps gene fragment"
     misc_feature    4553..4658
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 19'F' cps gene fragment"
     misc_feature    4659..4773
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 19C cps gene fragment"
     misc_feature    4774..4865
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 20 cps gene fragment"
     misc_feature    4866..4952
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 21 cps gene fragment"
     misc_feature    4953..5041
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 22AF cps gene fragment"
     misc_feature    5042..5127
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 23A cps gene fragment"
     misc_feature    5128..5205
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 23B cps gene fragment"
     misc_feature    5206..5431
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 23F cps gene fragment"
     misc_feature    5432..5551
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 24A cps gene fragment"
     misc_feature    5552..5684
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 24BF cps gene fragment"
     misc_feature    5685..5812
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 25AF cps gene fragment"
     misc_feature    5813..5891
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 27 cps gene fragment"
     misc_feature    5892..6032
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 28AF cps gene fragment"
     misc_feature    6033..6113
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 29 cps gene fragment"
     misc_feature    6114..6259
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 31 cps gene fragment"
     misc_feature    6260..6393
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 32AF cps gene fragment"
     misc_feature    6394..6498
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 33AF/37 cps gene fragment"
     misc_feature    6499..6644
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 33B cps gene fragment"
     misc_feature    6645..6791
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 33C cps gene fragment"
     misc_feature    6792..6872
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 33D cps gene fragment"
     misc_feature    6873..7016
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 34 cps gene fragment"
     misc_feature    7017..7124
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 35AC/42 cps gene fragment"
     misc_feature    7125..7248
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 35B cps gene fragment"
     misc_feature    7249..7393
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 35F/47F cps gene fragment"
     misc_feature    7394..7541
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 36 cps gene fragment"
     misc_feature    7542..7619
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 38 cps gene fragment"
     misc_feature    7620..7762
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 39 cps gene fragment"
     misc_feature    7763..7876
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 41A cps gene fragment"
     misc_feature    7877..8010
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 41F cps gene fragment"
     misc_feature    8011..8160
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 43 cps gene fragment"
     misc_feature    8161..8248
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 45 cps gene fragment"
     misc_feature    8249..8381
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 46 cps gene fragment"
     misc_feature    8382..8502
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 47AF cps gene fragment"
     misc_feature    8503..8635
                     /note="real time PCR target sequence - Streptococcus
                     pneumoniae serotype 48 cps gene fragment"
     primer_bind     complement(8652..8668)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8676..8692
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8700..8730)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8745..8766)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9054..9642)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9816..10673)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10674..10778)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtttgac aattaatcat cggctcgtat
      421 aatgtgtgga attgtgagcg gataacaatt tcacacgaat tcgtgcgttt taattccagc
      481 taaactccct gtatcaagcg ttttcggcaa acctgcttca tctgctagat tgcgttttca
      541 tccctatgtg tggtatagta gcttctttac tatttattct atttcttacc cgctactcta
      601 tagaatggag tatataaact atggttatac aaaagatttg attttcatgc aatggctggc
      661 tttggcaact ttgttgttaa ctctaccttc taaagctgtt atcccatata agaaccgagt
      721 gttaaattta gtttataaaa cacttattgc aatttcaatt tttttgcccc aatctcgtat
      781 agtttggata gcttttgggg taattttcca ctaaagcttt ggcaaaagaa atgcttccaa
      841 ttgtagaccg ccccacaatt cattttgtca ttgaagaagc tttacgttcg gggcttctgc
      901 tgtaactgtt gtgcctagta gcattccata tgaatatggg atgacatttc tacgcactat
      961 cctatcaatt tttcctattg gatggttagt tggtgatttt tttgataaag catcagcgac
     1021 ggttgttatt aataaatttt tggggttacc tgtaggctct tcttttgtgg aagaattatt
     1081 ttggaacttt ggttactatg gtggtgcatg atttatgccc tcttgcaacg ttcttcttct
     1141 catcgtttcc gcatgctttt tttagccctt gctttttctt atactgtcgt ttgcactaga
     1201 gtatgggaag gtgttgttct gccctgagca actggtcttg tatcgaagac atggacataa
     1261 tgtaacaact agtcatcatt ttaaattatc tccgctaaat gttttcagaa aggctattgg
     1321 gatgattggt cgtattagtg aacagaaaaa tcctttcttt tttgttgaat ttgcaaaaaa
     1381 attatcagag atttatagca atttatattt tggtattgtc ggcgatggcg aattgcgtgg
     1441 gcgaactgaa gaactaattg aagagatgaa ggctttggtt tgacaggagc agagagtatg
     1501 gcttgtggct gtgcgttagt ctcaacagcc tatagtggtg tatttgaata tgcaattgat
     1561 ggcgagaatt ccagatatag tcattcccaa tcaggaagtg ataatttcaa tgtataatgt
     1621 tttgaaaaat agtgactttg ctatggttgg tgggtcagta atcgataatg agggaaatta
     1681 tagactatta aattcatcat gggatttacc ttctttagtt tgagcataac ggagcgatat
     1741 gttttaatat agatgttccg aatattggtc cagctcgagc gattgcttat gatatagctg
     1801 ctatcaataa agctataaaa ctagctaagg aaaataagga tgaagctccc actcatcagt
     1861 ttcccatatg tttcaagggt tctgatggca gatgggttgg gacgagtaac gttcgcttct
     1921 tcaattattg aaggtatcct atatactgct ttaggtaagt attctaatgt tataattcaa
     1981 ttatttgtaa cggcggtatt aagtagaata ttaacacctg aagagtatgg cgttgtagcg
     2041 gttgtcaatg tgtttttagt attctttcag atattggcag attcgcgtgg aattttctat
     2101 actgcaatag ggaagtattc gaatgtagtt attcaattac ttgtaacagc aattcttagc
     2161 cggattctct cacctgtaga atatggtatc gtagcagtag agaggcccta agaaaagatt
     2221 cgtttctgaa tggtcgtatt atcacaaagg aaaatggagg tcatggctca acaattaatc
     2281 gtggtatcca ggaagcaaag gggaagttct ttaaagttat tgatggggat gactgggaaa
     2341 tatgagattg gtaaggaata ttctggaatt ccagagaaac ggattccaat gcactcggta
     2401 acttatagaa catctatcct agttgagaat ggaattcgtt taagtgaaaa gaccgagtta
     2461 tggatgttct tattggcttg tttggaacaa tattcacagt cttgccctgt tctctaataa
     2521 tttatattat ttataaaaca aaaggttata aagggagtat attttttaca caatacagag
     2581 tggggttggg aaatggtttg gatatggttt gtttggcttt caatttaaac ttccgtggca
     2641 gaaattggga gaagttggaa taaattacac tcataatcaa gttttacagt tagcacttca
     2701 aatttggcgt attgcttatc aaaaattttt agattcaaaa atatatggct atggagtttt
     2761 tagctttcat ttcaatcttc cgtggcaaga ttctggtgct aaggggatca actatgctca
     2821 taatcatggt ccagctactt ttatggcaat tattgctaaa ggaaaaactc caatagttgt
     2881 tccgaggcaa aagaaatttg gggagcatgt gaatgatcag cacccacggg taaatattct
     2941 acaacctgga cctggtgttg gtgggcattg tatttctgtg gatccttggt tcttagttga
     3001 gactaccatt ttttgatcag ttagatttaa tattaacagg tagattacga cttggaagtg
     3061 ctgcttttaa taattttcac ataacatttt ttggacaaaa aataagatgg atttatgaac
     3121 aaaatatgtt ttctgagagt gtatgaggaa tccctaaaag ctattaatgg aatggaaatg
     3181 ttacttggcg caggtgtcag aattccctct acagtagata tataattgcc tataaactca
     3241 ttgagatagt tttacctgat aatttgtata ctttttttaa tgttacaaag gcattatcat
     3301 atgagaatag gacagagttt gcgggacgaa caaatactat ttccttccta tggttgaatc
     3361 aggtagattg atttctgcta tgtctccggc ttttgtcttc tctgtattag gaaatatcat
     3421 taggactcag tatttgattc ctagaggcta gcaggaactt ttctaggggc tatacgccat
     3481 aatggattca tcccgtggga cgatgatatg gattttggta tgccaagaga ggattttgaa
     3541 agatttaaag aattatgtgt ttcggatttg gacagggata atgttatgac cttggtaatc
     3601 ttccccttaa tttttttgat tgttttagtt atattttatc tttatcctga gaatgttatt
     3661 ataaatatgc tctatagcag ttattcttca acacttaatt tgacagattc ttcagcggtt
     3721 aagcatttgg aagacttatg gcttcctctt ctaaaagtta ttgattatcc tttgggatga
     3781 ttatgtcatt cgattgcttg gaaatattaa tggttattta caacctacgg taacccacgc
     3841 ccataatgag tttttacaag ttgcttttca aacaggaatt ttaggatttt gcttttgtgg
     3901 gtaggacaag ctaatatttt accattttca gtcgttgttg ctctctgttt ttacttttat
     3961 gttcttgctg caggtgatat gcggagccaa tatattgacg cctcaggttt atgggataat
     4021 cgatggctag aacagattta tggaagggag ttgaatcaac ctataatttc gcccctatat
     4081 ttatggggag agggataggg tttgtaacaa aatggatgga taataattgg atgactttga
     4141 atatcaatgg tcttacaggg acaatggcgc ctagtctaaa taccaagatt aaagtgacgg
     4201 atcggctcat tgatatccaa ttctggaaaa tagctcttac tattatagtt gacctcggtc
     4261 atgcgagata cgacagaaag acctgaagga gtagctgctg gaacgttgaa attggttggg
     4321 actgatgagg atgaggttaa gattgctgat cgattaattg atatccaatt ttggaaaatt
     4381 gctctaacta ttataattga tctctttatt ttgtatcttt acaggaagga aatatacaac
     4441 cttgttctta gtaatggata tacggggtca aatattcagt ggttttttag aaatgcaacg
     4501 agttatgaag gtgaattgac agtgcgaact tttattcgag ttctcattcg tggtccttag
     4561 ttctggttat tcgggttcaa atattcagtg gttctttaga aattctacca gttatgaagg
     4621 tgagctaaca gtgcgaactt cgattcggtt cctcatccaa tggttttcag attacttgat
     4681 agctcaacaa ggacttccag ttgaaaaagt tcattatgtg ggggcaggaa caaatatgaa
     4741 taatctattt cttgaccact ctcataagga acgaaagata ctggctgagg agctatctat
     4801 tcaggataag gtctactttg tgggagttca gtcgaatata tcagaatggt tgagtacttt
     4861 tgactggttt aaatatcgct ccgggtataa ttgaagaaag aggtgtgaat tggacacgtt
     4921 atggagcggt tcgtctacaa gccctttttt tgtctattaa ataacccatt ggaattgaaa
     4981 cggcttggag aatgtgccca taagcgaatt acggaacagt ttatgtggtc ttcaattgcg
     5041 actcccctcc attacccatt tggtatggga gtagggagtg tgggagttct agcttcaaaa
     5101 ttaggttcac aaacagcact ttcttcattg aagaaattgc tccagaaaca tatagagcta
     5161 tttatctttc gtggtttttg ttagtggttg aggctagtct tttgggacag caacgacaat
     5221 agtcatctct ttactggcgt taacattttt tttcaaacat ataattaata atagtgaatc
     5281 atatagccat cgagtgttag gtgttgtgaa tttttttaaa tattatgaat cagataggtt
     5341 tcatttgttt tttggggatg ctgaattagc ctttggaaat acgacgaagg gttatggaca
     5401 caatattaga agtgtgttag gttgggatgg acttggagtt gctaattatg ggaagtatac
     5461 atttagtatg tctgttgtaa attacatctc ttatatagca gctgcgggta ttttacgata
     5521 tgctgtgcga gagtgtgcac gtgtaagaga ttctgaaagt aattagtaag attaacggaa
     5581 gaaagaaagt aatttattgg ttgctgacaa ttttgggact gtggatgatt actctaacac
     5641 ctatttttga aaatattgtt ggagctattt taaaagtaga tggaatacca actagaatca
     5701 gcaggacttt atttatgctt acccatcaag taaagataag ataatagata gtcccgctgg
     5761 acttactgca atactcgagt ttgcgagtaa attatcaaaa gatattccat ttagcgattt
     5821 agcgactgat atccaacaaa agcatatgtg gaaggcgttt gaaggtgact cacgcgagat
     5881 cgattttgag acaactacag gtatttttga tatcggagta gaaaatagta ggttgattgg
     5941 cggtgctttt ttaattaacc tttggcatgg cattcctttg aaaaaaatca tgtatgatga
     6001 taaacattca gcgcttcaca aacgtagtaa acttcgagtt gtgccgtttt tacaaaggag
     6061 tacgcagaga aaagactagg attcaaagga aaaattttag aggtgggtac gccgcagaag
     6121 ttttaagtca cggacatatt aatttatttg tgaatcatta tactttggcg cagaaaatag
     6181 aaaccttaaa taaggttggg acaaaccttg cggttttacg tggggggttt gaaaatccct
     6241 tagtgacatc tgtaatgctg tacttcctgt tctaggcttg ggagcagtgg ttgggtttga
     6301 acaatttaaa acttattttt tgacttctga tactccaagg ctcacattat ttaaatacgg
     6361 tatgcttaca atgagacgct attttcctct gggggaactg gttcagcaac tatacgcttt
     6421 gatgattata aggctggttt tttggcatgg cagaaaagtc ctatttgggg attaggtatt
     6481 tcagacggtc ttagaacccc tgttagtgca cctgtattta actcaggtgt attatatatt
     6541 gatttagata aatggcgtga acaacgaatc gaggaaaaat tagtttatat aataaataaa
     6601 tttaaaggaa atgtaattca aggagatgaa ggagttttga atgccagaga cagtttcagc
     6661 aaatcttaga gttgatgatt ataatattgg ttttatagta tggaagacaa gtttgtggat
     6721 aggacacgga ttgaataatg gtatattagc aattcaatca catattccaa cgtttataag
     6781 aggtgtaggc tcgtatagtc ttgcgacatt tcacaactag ttttttatca aaaagacctt
     6841 ggcaagaaag tagtgaggta acgcatgtgg aacggtggag taggtcaaga tgggattatt
     6901 gaagctctta atcgagaact attcaagtac attggcgctc cgttattaaa ttttaacaat
     6961 tttattgctg gatatgatcc gtatagaagt gactcagtat attggggaga acagacgctt
     7021 cccctttaga ctattcggtt actaaaatca gtaattggat gttatattca aaatacccag
     7081 gacacccgtt catattatcg atacgtgata ctttgatttc atttgcatgg aggtggagca
     7141 tacaaagata gtgacaattt aaacaatatt agtaaagcgc aggtcaagca aattaaaaag
     7201 agtgcagagt tggaagatta ttttatatcg agttgtgcag tctttacagt ggtcgtatat
     7261 acttgatgaa taaatcgttg aatttgtatg gttttggatt attcggacga ccagttgaat
     7321 ggaatggaaa tggtctgacc attgaaggag ttagaaacta tcagacctat ctgtatgttg
     7381 ataatttgta tgtcttgtct attcagccct tctggtttta ttagttaatg tagatatgaa
     7441 agtagttttg aaaacgtatc tcattgtagc gggcattctt gtactaagtg tatttctttt
     7501 atctctctta ggtgcagttc ccaatttaca atataatcgc ggtcttacgt agaacctctc
     7561 tggatgaatt gccacagatt tggaatattt tggtcggtca catgtcgctt gtaggaccac
     7621 aaaaaaatga actaactcaa atagtaacga agtcaggcgt attcttcaca agggaaatac
     7681 caatttattg gggagattat tctttagttg aagcagaaat agaacttttt gaaaccgcaa
     7741 ataaacaaga aaattacagt atgcaaatag atgtatccca gttaacaccc tttgaagcta
     7801 gactattcgg tcgtgttagt gaattattat ttaatgtgtg gttaaaacat aaggacatta
     7861 aaccaaaaga gctacctttt tgggaggaag tgcttttatt tcttctgtgc taacagtgga
     7921 gatattaagt ttaacaattc tattctctaa tttgaatgct ttattaatta cacctatttt
     7981 aacagttatg aatcatgaga aagcggtttt agaggctaca tcaaatagtt ggcattagat
     8041 atctatgctc ggtaggggtg agtactattg gaattgttct tgtaaagaaa ttacaattaa
     8101 gtgatttttt gttattatgt gtggaaggaa tcattttctt tggaagttac ggtgtgattc
     8161 tctagctact tgactaaaat atttgaactg ctagggagcg aggtcactaa aagtcgtgtt
     8221 atgtattata cagcgaaatc gactcgtccg aagtttttat atctctattg gtttgtattt
     8281 ttattttaat tttttattca actcgctttt tttattcgac taatgtagag ttgtatcatt
     8341 ctttcttcaa ttcctttctg attcgtccag ttcctgggat aaggaattgg tagagagttt
     8401 gtggatagat atttttccac ttgatggaat gcctgctgcc ccctttaaga gaaaaatgca
     8461 tgagtataga tacttatata gacggatgat ggttcaactt tccaggtttt gcttcatatg
     8521 ggagcaacgc tgcttatgtg tattactctc ccctgtattt tcaatttggt tttcatcata
     8581 tttggggtct taatttagaa tatgggaata tagctaatga taacttttgg ccgatgcttg
     8641 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8701 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8761 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8821 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8881 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8941 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9001 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9061 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9121 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9181 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9241 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9301 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9361 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9421 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9481 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9541 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9601 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9661 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9721 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9781 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9841 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9901 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagatcca
     9961 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10021 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10081 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10141 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10201 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10261 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10321 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10381 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10441 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10501 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10561 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10621 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10681 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10741 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10801 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    10861 aggccctttc gtc
//
