pNTAP-TSR vector (V004267)

MSDS

Basic Vector Information

Vector Name:: pNTAP-TSR

Antibiotic Resistance:: Ampicillin

Length:: 3968 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Kamil JP, Coen DM.

Promoter:: T7

pNTAP-TSR vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNTAP-TSR vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_33567        3968 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNTAP-TSR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Kamil JP, Coen DM.
  TITLE     Human cytomegalovirus protein kinase UL97 forms a complex with the 
            tegument phosphoprotein pp65
  JOURNAL   J. Virol. 81 (19), 10659-10668 (2007)
  PUBMED    17634236
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Kamil JP, Coen DM.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 3968)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3968)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "2007"; volume: "81"; issue: "19"; pages: "10659-10668"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUL-2007) Biological Chemistry "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             61..234
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     misc_feature    235..262
                     /note="partial IgG binding domain 2; Staphylococcus aureus 
                     protein A gammaglobulin binding domain; partial repeat for 
                     Red-recombination substrate"
     misc_feature    263..280
                     /label=I-SceI homing endonuclease recognition site
                     /note="I-SceI homing endonuclease recognition site"
     CDS             404..1216
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    1251..1272
                     /note="partial IgG binding domain 1; Staphylococcus aureus 
                     protein A gammaglobulin binding domain; partial repeat for 
                     Red-recombination substrate"
     CDS             1273..1446
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     misc_feature    1462..1482
                     /label=spacer region
                     /note="spacer region"
     CDS             1483..1503
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    1504..1509
                     /label=spacer region
                     /note="spacer region"
     misc_feature    1510..1545
                     /note="protein C epitope; binds anti-protein C monoclonal 
                     antibody (Roche)"
     promoter        complement(1606..1624)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1882..2470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2644..3501)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3502..3606)
                     /label=AmpR promoter
     promoter        3952..3968
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"

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