LOCUS       40924_33567        3968 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNTAP-TSR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Kamil JP, Coen DM.
  TITLE     Human cytomegalovirus protein kinase UL97 forms a complex with the 
            tegument phosphoprotein pp65
  JOURNAL   J. Virol. 81 (19), 10659-10668 (2007)
  PUBMED    17634236
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Kamil JP, Coen DM.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 3968)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3968)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "2007"; volume: "81"; issue: "19"; pages: "10659-10668"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUL-2007) Biological Chemistry "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             61..234
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     misc_feature    235..262
                     /note="partial IgG binding domain 2; Staphylococcus aureus 
                     protein A gammaglobulin binding domain; partial repeat for 
                     Red-recombination substrate"
     misc_feature    263..280
                     /label=I-SceI homing endonuclease recognition site
                     /note="I-SceI homing endonuclease recognition site"
     CDS             404..1216
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    1251..1272
                     /note="partial IgG binding domain 1; Staphylococcus aureus 
                     protein A gammaglobulin binding domain; partial repeat for 
                     Red-recombination substrate"
     CDS             1273..1446
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     misc_feature    1462..1482
                     /label=spacer region
                     /note="spacer region"
     CDS             1483..1503
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    1504..1509
                     /label=spacer region
                     /note="spacer region"
     misc_feature    1510..1545
                     /note="protein C epitope; binds anti-protein C monoclonal 
                     antibody (Roche)"
     promoter        complement(1606..1624)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1882..2470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2644..3501)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3502..3606)
                     /label=AmpR promoter
     promoter        3952..3968
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaactcgagc agctgaagct tgcatgcacc atggcaggcc ttgcgcacca cgatgaagcc
       61 gtggataaca aattcaacaa agagcagcag aacgccttct acgagatcct ccatctgccc
      121 aacctgaacg aggagcagag gaatgccttc atccagagcc tgaaggacga ccctagccag
      181 agcgccaatc tgctcgccga ggccaagaag ctgaacgacg cccaggcccc caaggtcgac
      241 aataagttta ataaggaaca gctagggata acagggtaat cgatttattc aacaaagcca
      301 cgttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
      361 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
      421 ggaaacgtct tgctcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
      481 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa
      541 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
      601 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
      661 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc
      721 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
      781 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
      841 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
      901 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataagctttt
      961 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     1021 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     1081 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     1141 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     1201 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc gaacgacgcc
     1261 caggccccca aggtcgacaa taagtttaat aaggaacagc agaatgcttt ttatgaaatt
     1321 ctccacctcc ccaatctcaa tgaagaacag cggaacgcct ttatccagtc cctgaaagat
     1381 gatcccagcc agtctgccaa cctgctggct gaagctaaaa aactgaatga tgctcaggct
     1441 cctaaggtgg acgctaattg tgatatccct accaccgcca gcgagaacct gtacttccag
     1501 ggcgagctgg aagatcaggt cgatcctcgt cttattgatg ggaaatctag aggatccccg
     1561 ggtaccgagc tcgaattcat cgatgatatc agatctgccg gtctccctat agtgagtcgt
     1621 attaatttcg ataagccagg ttaacctgca ttaatgaatc ggccaacgcg cggggagagg
     1681 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1741 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1801 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1861 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1921 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1981 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2041 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2101 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2161 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2221 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2281 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2341 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2401 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2461 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2521 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2581 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2641 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2701 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2761 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     2821 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2881 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2941 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3001 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3061 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3121 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3181 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3241 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3301 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3361 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3421 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3481 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3541 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3601 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     3661 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     3721 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     3781 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     3841 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgg acatattgtc
     3901 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
     3961 acactata
//
