pNS-2xMyc vector (V004277)

LOCUS       40924_33507        7830 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNS-2xMyc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Ha H-J.
  TITLE     Construction of a thiamine-repessible vector for yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Ha H-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2016) Department of Horticulture, Chungnam 
            National University, 99 Daehak-ro, Daejeon, Yuseong 305-761, 
            Republic of Korea
REFERENCE   3  (bases 1 to 7830)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7830)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-FEB-2016) Department of Horticulture, Chungnam National 
            University, 99 Daehak-ro, Daejeon, Yuseong 305-761, Republic of 
            Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            280..1399
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        1457..2613
                     /label=nmt1 promoter
                     /note="wild-type nmt1 promoter from Schizosaccharomyces
                     pombe, conferring strong thiamine-repressible expression"
     CDS             2627..2656
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             2660..2689
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      2757..2898
                     /label=Tnmt1
                     /note="Tnmt1"
                     /regulatory_class="terminator"
     rep_origin      complement(3825..4609)
                     /direction=LEFT
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(4959..4975)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5188..5643
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5925..6029
                     /label=AmpR promoter
     CDS             6030..6887
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7061..7649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"