Basic Vector Information
- Vector Name:
- pNS-2xMyc
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7830 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Ha H-J.
- Promoter:
- nmt1
pNS-2xMyc vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNS-2xMyc vector Sequence
LOCUS 40924_33507 7830 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNS-2xMyc, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7830)
AUTHORS Ha H-J.
TITLE Construction of a thiamine-repessible vector for yeast
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7830)
AUTHORS Ha H-J.
TITLE Direct Submission
JOURNAL Submitted (15-FEB-2016) Department of Horticulture, Chungnam
National University, 99 Daehak-ro, Daejeon, Yuseong 305-761,
Republic of Korea
REFERENCE 3 (bases 1 to 7830)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7830)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(15-FEB-2016) Department of Horticulture, Chungnam National
University, 99 Daehak-ro, Daejeon, Yuseong 305-761, Republic of
Korea"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..7830
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 107..128
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
gene 280..1399
/label=natMX6
/note="yeast selectable marker conferring nourseothricin
resistance"
promoter 1457..2613
/label=nmt1 promoter
/note="wild-type nmt1 promoter from Schizosaccharomyces
pombe, conferring strong thiamine-repressible expression"
CDS 2627..2656
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
CDS 2660..2689
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
regulatory 2757..2898
/label=Tnmt1
/note="Tnmt1"
/regulatory_class="terminator"
rep_origin complement(3825..4609)
/direction=LEFT
/label=ars1
/note="Schizosaccharomyces pombe autonomously replicating
sequence ars1"
primer_bind complement(4959..4975)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 5188..5643
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5925..6029
/label=AmpR promoter
CDS 6030..6887
/label=AmpR
/note="beta-lactamase"
rep_origin 7061..7649
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
This page is informational only.