LOCUS       40924_33507        7830 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNS-2xMyc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Ha H-J.
  TITLE     Construction of a thiamine-repessible vector for yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Ha H-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2016) Department of Horticulture, Chungnam 
            National University, 99 Daehak-ro, Daejeon, Yuseong 305-761, 
            Republic of Korea
REFERENCE   3  (bases 1 to 7830)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7830)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-FEB-2016) Department of Horticulture, Chungnam National 
            University, 99 Daehak-ro, Daejeon, Yuseong 305-761, Republic of 
            Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            280..1399
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        1457..2613
                     /label=nmt1 promoter
                     /note="wild-type nmt1 promoter from Schizosaccharomyces
                     pombe, conferring strong thiamine-repressible expression"
     CDS             2627..2656
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             2660..2689
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      2757..2898
                     /label=Tnmt1
                     /note="Tnmt1"
                     /regulatory_class="terminator"
     rep_origin      complement(3825..4609)
                     /direction=LEFT
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(4959..4975)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5188..5643
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5925..6029
                     /label=AmpR promoter
     CDS             6030..6887
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7061..7649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttt
      241 gtttagcttg cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc
      301 ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt
      361 tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg
      421 cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca
      481 gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg
      541 ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac
      601 atcacatccg aacataaaca accatgggta ccactcttga cgacacggct taccggtacc
      661 gcaccagtgt cccaggggac gccgaggcca tcgaggcact ggatgggtcc ttcaccaccg
      721 acaccgtctt ccgcgtcacc gccaccgggg acggcttcac cctgcgggag gtgccggtgg
      781 acccgcccct gaccaaggtg ttccccgacg acgaatcgga cgacgaatcg gacgacgggg
      841 aggacggcga cccggactcc cggacgttcg tcgcgtacgg ggacgacggc gacctggcgg
      901 gcttcgtggt cgtctcgtac tccggctgga accgccggct gaccgtcgag gacatcgagg
      961 tcgccccgga gcaccggggg cacggggtcg ggcgcgcgtt gatggggctc gcgacggagt
     1021 tcgcccgcga gcggggcgcc gggcacctct ggctggaggt caccaacgtc aacgcaccgg
     1081 cgatccacgc gtaccggcgg atggggttca ccctctgcgg cctggacacc gccctgtacg
     1141 acggcaccgc ctcggacggc gagcaggcgc tctacatgag catgccctgc ccctaatcag
     1201 tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat
     1261 tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat
     1321 catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg
     1381 aatgctggtc gctatactgc tgtcgattcg atactaacgc ctgcaggtcg atcgactcta
     1441 gaggatcaga aaattatcgc cataaaagac agaataagtc atcagcggtt gtttcatttc
     1501 ctatattttt tttttatttt tttatttttt aataagggaa aatttaacgt ctaaggatac
     1561 agaagattgt tagcacatta aagtaataaa ggcttaagta gtaagtgcct tagcatgtta
     1621 ttgtatttca aaggacataa tctaaaataa taacaatatc atttctcaca agttattcaa
     1681 ttttcttttt tttttctaat aatatcaaga atgtattatt tgtttgacat aagtcaacta
     1741 atttatttaa tatgctggat taatcttgca gacatgtaaa ttaacaagtt ttagtcaaat
     1801 aacgttgaag tttcaatgaa ctcaaataat ttctcttttt ttttatataa ccataatctg
     1861 atttatattt tccgcaggga tcaactgaag ttatgacatt tggattggat cacttataac
     1921 cttggtcgcc aaataataca aaaatcagcg ttataaaaca aagaaggttt ttgttaagaa
     1981 attaatcctc tttcttgata agaaagttga accgaaattg cagatactga tatatgaaaa
     2041 taatacccac aattttggga atagcgcaag cctcaattta aacaataggt gaggacacat
     2101 gataatgacc tcaatgattg ttagaagaaa agagcctcat tacaaaatcg aaaaatgaat
     2161 ggttgggtac aagtttccaa aacatggtaa agtggacttt gcgtatgaga cgtaaataga
     2221 aaaaaacact tgttatatgt tttctagaat tattgttgtc tctttatggt tggatgatgc
     2281 aaaatagtaa tttcggttag ttgctgtaaa acaccacgag acaaatagat atggatattt
     2341 attaaatcag gaaaaacgta actctcggct actggatggt tcagtcaccc aacgattact
     2401 ggggagagaa aacagggcaa aagcaaagct taaaggaatc cgattgtcat tcggcaatgt
     2461 gcagcgaaac taaaaaccgg ataatggacc tgttaatcga aacattgaag atatataaag
     2521 gaagaggaat cctggcatat catcaattga ataagttgaa ttaattattt caatctcatt
     2581 ctcactttct gacttatagt cgctttgtta aattggcctc gagatggagc agaaattgat
     2641 cagcgaggaa gacttgggcg aacaaaagct tatttctgaa gaagacttgc ttgcggccgc
     2701 gaatagatct gatatcggcg ccagtcgact ctagaggatc cccgcggccc gggtaaaagg
     2761 aatgtctccc ttgccagtac tgctagggtt tttctttcaa actatggaag cccattcaag
     2821 ctgcatatta cgattttgtt tttcgctttt agaaagtggt ttagatgaga taatagaaaa
     2881 attcttgatc tccgacaacg agtactttta ttttttttgc taatcacttt actcaatatt
     2941 agctcgaaat cgtagaaacg tagacgggtg cgggataccg agtggtgtag ttaagaattt
     3001 ttataaacca cgtggcccaa aaatatgaac ccaaaacgtt tatacatgag tatactttaa
     3061 gaaggctata ccccttcgtg ttagatgtag ttttagctac ccaacccgag tctatgagct
     3121 tgacttcaga tgtagaaggc attaaatcgt tttgaatatt aattaaaaaa cgatgaaaat
     3181 taaatattta aaagcaatca tacgctgaaa atttagtgct gtggctaatc cttcaacatg
     3241 gaaatgccat aaaagtgact ttgacaaaaa aaaaagtata tacaggtagt aaactcatct
     3301 acttcattga ctttgtttac agcatgtgga aggaggaata tttattgcta aatcgtagtt
     3361 taacattcaa taagtaatac tattgaaatt cgacaagatt ggccgcatgg atgaaaaaga
     3421 ggcattttgc tttgggagaa ttagttcaaa ttagaactga aaaaaaaaac tttacgaggc
     3481 aaaaatgtcg gattgagatc gtaaaagttc gctcgtcgtc ttttgctttg tgattgtttt
     3541 catggataca tcttgctgga tatttaaatt ttagtactat gtataagata ttctataaat
     3601 gttttatcac ccaaacctgt tagcgccttc ttaattctat tcaatctggc ttttgctctg
     3661 agactacttc ttggactttc actacttgtt agttatacgg aatttgtgta attagaagtg
     3721 aaataatcct ttctattagt aatgcgagct cgaattcgag tctaactcct taaccactcg
     3781 gacatagtga cttatctgac actcattgta aattaatata tatataggca ttttgtttag
     3841 ttaaaggtac ttaagtaatt agtataaacg aaccaatttt ataatcagga agttaagtga
     3901 atggtagcac atgtcgtaaa aattgtgaat ttttattgaa taatatttta aatacaagcc
     3961 tttctagact aggtatactc ataaacatat atgagcaaaa ggatagagga gattacattg
     4021 catcttctac aaatttattt attgcccttt actgaaaaat taaataatga gtactaaatg
     4081 ataaaaagcg ctcagtacaa gaaagattgc aaaaatattg cattcttcat gaattaaagt
     4141 tgcatataag gcatattgaa agtaatagta ctaaaacagc agttagcgaa aattaataga
     4201 attatattcg caagacaatt gtgacattaa attaaaaaat tgtagaattt ttactatcct
     4261 ctttaacgcc atgagccttt ataaaaaggt taaattagtt ttaacattct ttttttgagt
     4321 aagagtttac aatttatcaa aacctgtgtt attatattca ttagtttcaa tttattagca
     4381 tctagagaaa aatcaattgg cagttacatt gttggaattt atgaagaaaa agaatctaca
     4441 acggagaata agttgctgat cgctttccct aaaattgtat attttgctga gcttattttg
     4501 acatttcgtt gaagttttct ctaattcgca ttcatttnta agtaaaacaa tgagaaataa
     4561 aattacaaaa aatacaaatt aaaatacaat ttttagctat aattatagac gatgcccttg
     4621 tatcccattc tgtctcgctt gcccctactt tttatctttt atataccata atgaacgctg
     4681 ccgctactaa ccataccccg attttacatt tcggactccc aaggacgtac aaaatagaaa
     4741 actatagaaa aaaataatca gaaaatagca tgtcatctct ttgtaaaacg cgtttgcaag
     4801 aagaaaggaa acaatggaga agagatcatc catttgtatg taaattttag taaacttgaa
     4861 gaaatcacta acaacttctc ttacttaggg attctatgca aaaccttgta aatcatctga
     4921 tggaggactc gatttaatga attggaaggt tggaattcac tggccgtcgt tttacaacgt
     4981 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5041 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5101 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     5161 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     5221 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5281 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5341 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5401 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5461 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5521 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5581 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     5641 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     5701 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5761 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     5821 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     5881 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5941 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6001 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6061 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6121 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6181 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6241 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6301 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6361 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6421 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6481 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6541 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6601 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6661 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6721 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6781 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6841 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6901 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6961 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7021 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7081 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7141 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7201 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7261 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7321 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7381 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7441 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7501 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7561 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7621 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7681 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7741 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7801 gcagcgagtc agtgagcgag gaagcggaag
//
