pNRSAL vector (V004278)

LOCUS       V004278                 6069 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004278
VERSION     V004278
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Park HH, Lim WK, Shin HJ.
  TITLE     In vitro binding of purified NahR regulatory protein with promoter
            Psal
  JOURNAL   Biochim. Biophys. Acta 1725 (2), 247-255 (2005)
   PUBMED   15978733
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Park HH, Lim WK, Shin HJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2002) Dongseo University, San 69-1, Jurye -2-dong,
            Sasang-gu, Busan 617-716, Korea
REFERENCE   3  (bases 1 to 6069)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6069)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim.
            Biophys. Acta"; date: "2005"; volume: "1725"; issue: "2"; pages:
            "247-255"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-MAY-2002) Dongseo University, San 69-1, Jurye -2-dong,
            Sasang-gu, Busan 617-716, Korea"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(236..1135)
                     /gene="nahR"
                     /label="HTH-type transcriptional activator NahR"
                     /note="HTH-type transcriptional activator NahR from
                     Pseudomonas putida. Accession#: P10183"
     regulatory      complement(1136..1225)
                     /label="Pr promoter"
                     /note="Pr promoter"
                     /regulatory_class="promoter"
     regulatory      1214..1282
                     /label="Psal promoter"
                     /note="Psal promoter"
                     /regulatory_class="promoter"
     CDS             1339..2988
                     /label="luciferase"
                     /note="firefly luciferase"
     polyA_signal    complement(3032..3153)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3572..4160)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4334..5191)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5192..5296)
                     /label="AmpR promoter"
     rep_origin      5323..5778
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5909..5957
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    5971..6062
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"