Basic Vector Information
- Vector Name:
- pNRSAL
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6069 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Park HH, Lim WK, Shin HJ.
pNRSAL vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNRSAL vector Sequence
LOCUS 40924_33502 6069 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNRSAL, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6069)
AUTHORS Park HH, Lim WK, Shin HJ.
TITLE In vitro binding of purified NahR regulatory protein with promoter
Psal
JOURNAL Biochim. Biophys. Acta 1725 (2), 247-255 (2005)
PUBMED 15978733
REFERENCE 2 (bases 1 to 6069)
AUTHORS Park HH, Lim WK, Shin HJ.
TITLE Direct Submission
JOURNAL Submitted (23-MAY-2002) Dongseo University, San 69-1, Jurye -2-dong,
Sasang-gu, Busan 617-716, Korea
REFERENCE 3 (bases 1 to 6069)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6069)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biochim.
Biophys. Acta"; date: "2005"; volume: "1725"; issue: "2"; pages:
"247-255"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(23-MAY-2002) Dongseo University, San 69-1, Jurye -2-dong,
Sasang-gu, Busan 617-716, Korea"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6069
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(236..1135)
/gene="nahR"
/label=nahR
/note="HTH-type transcriptional activator NahR from
Pseudomonas putida. Accession#: P10183"
regulatory complement(1136..1225)
/label=Pr promoter
/note="Pr promoter"
/regulatory_class="promoter"
regulatory 1214..1282
/label=Psal promoter
/note="Psal promoter"
/regulatory_class="promoter"
CDS 1339..2988
/label=luciferase
/note="firefly luciferase"
polyA_signal complement(3032..3153)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3572..4160)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4334..5191)
/label=AmpR
/note="beta-lactamase"
promoter complement(5192..5296)
/label=AmpR promoter
rep_origin 5323..5778
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal 5909..5957
/label=poly(A) signal
/note="synthetic polyadenylation signal"
misc_feature 5971..6062
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
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